Journal ArticleDOI
Letter: A new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by quinacrine fluorescence and Giemsa staining.
TLDR
An unsuspected abnormality in all cells from the nine patients with chronic myelogenous leukaemia has been detected with quinacrine fluorescence and various Giemsa staining techniques, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and thelong arm of 9, producing the 9q+ chromosome.Abstract:
CELLS from nine consecutive patients with chronic myelogenous leukaemia (CML) have been analysed with quinacrine fluorescence and various Giemsa staining techniques. The Philadelphia (Ph1) chromosome in all nine patients represents a deletion of the long arm of chromosome 22 (22q−)1,2. An unsuspected abnormality in all cells from the nine patients has been detected with these new staining techniques. It consists of the addition of dully fluorescing material to the end of the long arm of one chromosome 9 (9q+). In Giemsa-stained preparations, this material appears as an additional faint terminal band in one chromosome 9. The amount of additional material is approximately equal to the amount missing from the Ph1 (22q−) chromosome, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and the long arm of 9, producing the 9q+ chromosome.read more
Citations
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Journal ArticleDOI
Chromosomes and causation of human cancer and leukemia. XXIV. Unusual and complex Ph1 translocations and their clinical significance.
TL;DR: In this paper, the authors evaluated the clinical, prognostic, and survival picture in 30 known cases of chronic myelocytic leukemia (CML) with unusual or complex Ph 1 translocations.
Journal ArticleDOI
Clinical targeting of mutated and wild-type protein tyrosine kinases in cancer.
TL;DR: Evidence supporting the common mechanisms of protein tyrosine kinase activation in cancer is summarized and a personal perspective on the kinases BCR-ABL and BTK, as well as nonmutated kinase targets in prostate cancer, are provided.
Journal ArticleDOI
BCR-ABL in Chronic Myelogenous Leukemia – How Does It Work?
John M. Goldman,Junia V. Melo +1 more
TL;DR: The discovery of the BCR-ABL fusion gene on the Philadelphia (Ph) chromosome in 1985 was the start of a new era in understanding the molecular basis of hematologic malignancies and though some of the mutant Ph-positive subclones that develop in patients taking tyrosine kinase inhibitors are the direct cause of the resistance observed, in other cases, its cause is unclear.
Journal ArticleDOI
The biology of signal transduction inhibition: Basic science to novel therapies
TL;DR: The tyrosine kinase inhibitor imatinib mesylate was recently approved for the treatment of CML and provides proof of principle for the strategy of targeted signal transduction inhibition, showing that inhibition of a single oncogene in a multigene disorder also may be of benefit.
Journal ArticleDOI
Aneuploidy and malignancy: an unsolved equation
TL;DR: Evidence suggests that aneuploidy possibly plays an active role in carcinogenesis, with special emphasis on its mechanism of development and impact on progression of cancer.
References
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Journal ArticleDOI
New Technique for Distinguishing between Human Chromosomes
TL;DR: It seems probable, therefore, that the darker staining with Giemsa of these regions, after denaturation and annealing, indicates the presence of highly repetitive DNA.
Journal ArticleDOI
Technique for Identifying Y Chromosomes in Human Interphase Nuclei
TL;DR: This work investigated the possibility of positively identifying male nuclei in interphase by virtue of this staining property of the Y chromosome using quinacrine dihydro-chloride.
Journal ArticleDOI
Clinical Implications of Cytogenetic Variants in Chronic Myelocytic Leukemia (CML)
TL;DR: The development of other chromosomal abnormalities in Ph1 positive patients presaged the terminal stage of the disease.
Journal ArticleDOI
Philadelphia-Chromosome-Positive and -Negative Chronic Myelocytic Leukemia
TL;DR: Chromosomal studies were performed on 61 adult patients with "typical chronic myelocytic leukemia" and the Philadelphia (Ph1) chromosome was found in 43 patients, with equal sex distribution a year after diagnosis.
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