Journal ArticleDOI
Letter: A new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by quinacrine fluorescence and Giemsa staining.
TLDR
An unsuspected abnormality in all cells from the nine patients with chronic myelogenous leukaemia has been detected with quinacrine fluorescence and various Giemsa staining techniques, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and thelong arm of 9, producing the 9q+ chromosome.Abstract:
CELLS from nine consecutive patients with chronic myelogenous leukaemia (CML) have been analysed with quinacrine fluorescence and various Giemsa staining techniques. The Philadelphia (Ph1) chromosome in all nine patients represents a deletion of the long arm of chromosome 22 (22q−)1,2. An unsuspected abnormality in all cells from the nine patients has been detected with these new staining techniques. It consists of the addition of dully fluorescing material to the end of the long arm of one chromosome 9 (9q+). In Giemsa-stained preparations, this material appears as an additional faint terminal band in one chromosome 9. The amount of additional material is approximately equal to the amount missing from the Ph1 (22q−) chromosome, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and the long arm of 9, producing the 9q+ chromosome.read more
Citations
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Journal ArticleDOI
The spectrum of molecular alterations in the evolution of chronic myelocytic leukemia.
HG Ahuja,Menashe Bar-Eli,Zalmen A. Arlin,Suresh H. Advani,Steven L. Allen,John M. Goldman,David S. Snyder,A Foti,Martin J. Cline +8 more
TL;DR: It is concluded that heterogeneous alterations in the p53 gene and occasionally in the N-RAS genes accompany the evolution of chronic phase CML to blast crisis.
Journal ArticleDOI
LMO T-cell translocation oncogenes typify genes activated by chromosomal translocations that alter transcription and developmental processes
TL;DR: The scientific challenge of the last decade has been to define the contribution of the genes activated by translocations to the course of tumor development and to ascertain whether any general principles can be determined about these ‘translocation’ genes.
Journal ArticleDOI
Kinome-wide Decoding of Network-Attacking Mutations Rewiring Cancer Signaling
Pau Creixell,Erwin M. Schoof,Craig D. Simpson,James Longden,Chad J. Miller,Hua Jane Lou,Lara Perryman,Thomas R. Cox,Nevena Zivanovic,Antonio Palmeri,Agata Wesolowska-Andersen,Manuela Helmer-Citterich,Jesper Ferkinghoff-Borg,Hiroaki Itamochi,Bernd Bodenmiller,Janine T. Erler,Benjamin E. Turk,Rune Linding,Rune Linding +18 more
TL;DR: Six types of network-attacking mutations (NAMs) are identified, including changes in kinase and SH2 modulation, network rewiring, and the genesis and extinction of phosphorylation sites, and mutant molecular logic gates and kinase-inactivating hotspots in cancer are discovered.
Journal ArticleDOI
Chromosomal translocations in cancer
TL;DR: In this review, the well-studied chromosomal translocations in cancer are summarized and the mechanism of formation of most of these translocations is still unclear.
Journal ArticleDOI
Heterogeneity of chromosome 22 breakpoint in Philadelphia-positive (Ph+) acute lymphocytic leukemia.
J Erikson,Constance A. Griffin,Abbas Ar-Rushdi,Mauro Valtieri,James A. Hoxie,Beverly S. Emanuel,Giovanni Rovera,Peter C. Nowell,Carlo M. Croce +8 more
TL;DR: In this paper, the authors investigated the chromosome breakpoints in several cases of ALL carrying the t(9;22) translocation and found that the breakpoints on chromosome 22 occur within a 58-kilobase segment of DNA referred to as breakpoint cluster region.
References
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Journal ArticleDOI
New Technique for Distinguishing between Human Chromosomes
TL;DR: It seems probable, therefore, that the darker staining with Giemsa of these regions, after denaturation and annealing, indicates the presence of highly repetitive DNA.
Journal ArticleDOI
Technique for Identifying Y Chromosomes in Human Interphase Nuclei
TL;DR: This work investigated the possibility of positively identifying male nuclei in interphase by virtue of this staining property of the Y chromosome using quinacrine dihydro-chloride.
Journal ArticleDOI
Clinical Implications of Cytogenetic Variants in Chronic Myelocytic Leukemia (CML)
TL;DR: The development of other chromosomal abnormalities in Ph1 positive patients presaged the terminal stage of the disease.
Journal ArticleDOI
Philadelphia-Chromosome-Positive and -Negative Chronic Myelocytic Leukemia
TL;DR: Chromosomal studies were performed on 61 adult patients with "typical chronic myelocytic leukemia" and the Philadelphia (Ph1) chromosome was found in 43 patients, with equal sex distribution a year after diagnosis.
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