Journal ArticleDOI
Letter: A new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by quinacrine fluorescence and Giemsa staining.
TLDR
An unsuspected abnormality in all cells from the nine patients with chronic myelogenous leukaemia has been detected with quinacrine fluorescence and various Giemsa staining techniques, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and thelong arm of 9, producing the 9q+ chromosome.Abstract:
CELLS from nine consecutive patients with chronic myelogenous leukaemia (CML) have been analysed with quinacrine fluorescence and various Giemsa staining techniques. The Philadelphia (Ph1) chromosome in all nine patients represents a deletion of the long arm of chromosome 22 (22q−)1,2. An unsuspected abnormality in all cells from the nine patients has been detected with these new staining techniques. It consists of the addition of dully fluorescing material to the end of the long arm of one chromosome 9 (9q+). In Giemsa-stained preparations, this material appears as an additional faint terminal band in one chromosome 9. The amount of additional material is approximately equal to the amount missing from the Ph1 (22q−) chromosome, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and the long arm of 9, producing the 9q+ chromosome.read more
Citations
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Journal ArticleDOI
Identification of molecular variants of p210bcr-abl in chronic myelogenous leukemia
Razelle Kurzrock,William S. Kloetzer,Moshe Talpaz,Mark Blick,Ronald S. Walters,Ralph B. Arlinghaus,Jordan U. Gutterman +6 more
TL;DR: The observations demonstrate that the Philadelphia translocation results in the generation of a chimeric bcr-abl protein with at least two molecular variants, both of which are enzymatically active as protein kinases.
Journal ArticleDOI
Detection of Gene Rearrangements in Targeted Clinical Next-Generation Sequencing
Haley J. Abel,Hussam Al-Kateb,Catherine E. Cottrell,Andrew J. Bredemeyer,Colin C. Pritchard,Allie H. Grossmann,Michelle L. Wallander,John D. Pfeifer,Christina M. Lockwood,Eric J. Duncavage +9 more
TL;DR: It is reported that clinically relevant chromosomal rearrangements can be detected from targeted gene panel-based next-generation sequencing with sensitivity and specificity equivalent to that of FISH while providing finer-scale information and increased efficiency for molecular oncology testing.
Journal ArticleDOI
Correlation between molecular and clinical events in the evolution of chronic myelocytic leukemia to blast crisis
A Foti,HG Ahuja,S. L. Allen,Prasad Koduru,M. W. Schuster,P. Schulman,M. Bar-Eli,Martin J. Cline +7 more
TL;DR: Observations suggest that abnormalities of the p53 anti-oncogene are temporally related to the clinical progression of some cases of CML and are probably responsible for the development of blast crisis in these cases.
Journal ArticleDOI
Pilot study of lonafarnib, a farnesyl transferase inhibitor, in patients with chronic myeloid leukemia in the chronic or accelerated phase that is resistant or refractory to imatinib therapy.
Gautam Borthakur,Hagop Kantarjian,George Q. Daley,Moshe Talpaz,Susan O'Brien,Guillermo Garcia-Manero,Francis Giles,Stefan Faderl,Michael Sugrue,Jorge Cortes +9 more
TL;DR: Lonafarnib is a nonpeptidomimetic farnesyl transferase inhibitor that has demonstrated significant preclinical activity against chronic myelogenous leukemia (CML) cells and in CML animal models.
Journal ArticleDOI
Overexpression of P-glycoprotein induces acquired resistance to imatinib in chronic myelogenous leukemia cells.
TL;DR: Data suggest that the overexpression of P-gp may play a crucial role in acquired resistance to imatinib in CML K562-imatinib cells.
References
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Journal ArticleDOI
New Technique for Distinguishing between Human Chromosomes
TL;DR: It seems probable, therefore, that the darker staining with Giemsa of these regions, after denaturation and annealing, indicates the presence of highly repetitive DNA.
Journal ArticleDOI
Technique for Identifying Y Chromosomes in Human Interphase Nuclei
TL;DR: This work investigated the possibility of positively identifying male nuclei in interphase by virtue of this staining property of the Y chromosome using quinacrine dihydro-chloride.
Journal ArticleDOI
Clinical Implications of Cytogenetic Variants in Chronic Myelocytic Leukemia (CML)
TL;DR: The development of other chromosomal abnormalities in Ph1 positive patients presaged the terminal stage of the disease.
Journal ArticleDOI
Philadelphia-Chromosome-Positive and -Negative Chronic Myelocytic Leukemia
TL;DR: Chromosomal studies were performed on 61 adult patients with "typical chronic myelocytic leukemia" and the Philadelphia (Ph1) chromosome was found in 43 patients, with equal sex distribution a year after diagnosis.
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