Journal ArticleDOI
Letter: A new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by quinacrine fluorescence and Giemsa staining.
TLDR
An unsuspected abnormality in all cells from the nine patients with chronic myelogenous leukaemia has been detected with quinacrine fluorescence and various Giemsa staining techniques, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and thelong arm of 9, producing the 9q+ chromosome.Abstract:
CELLS from nine consecutive patients with chronic myelogenous leukaemia (CML) have been analysed with quinacrine fluorescence and various Giemsa staining techniques. The Philadelphia (Ph1) chromosome in all nine patients represents a deletion of the long arm of chromosome 22 (22q−)1,2. An unsuspected abnormality in all cells from the nine patients has been detected with these new staining techniques. It consists of the addition of dully fluorescing material to the end of the long arm of one chromosome 9 (9q+). In Giemsa-stained preparations, this material appears as an additional faint terminal band in one chromosome 9. The amount of additional material is approximately equal to the amount missing from the Ph1 (22q−) chromosome, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and the long arm of 9, producing the 9q+ chromosome.read more
Citations
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Journal ArticleDOI
Involvement of Fas-mediated apoptosis in the inhibitory effects of interferon-α in chronic myelogenous leukemia
Carmine Selleri,Tadatsugu Sato,Luigi Del Vecchio,Luigia Luciano,A. John Barrett,Bruno Rotoli,Neal S. Young,Jaroslaw P. Maciejewski +7 more
TL;DR: The findings suggest that the Fas-R/Fas-ligand system might be involved in the immunologic regulation of CML progenitor growth and that its effect can be amplified by IFN-alpha.
Journal ArticleDOI
Identification of heme oxygenase-1 as a novel BCR/ABL-dependent survival factor in chronic myeloid leukemia.
Matthias Mayerhofer,Stefan Florian,Maria-Theresa Krauth,Karl J. Aichberger,Martin Bilban,Rodrig Marculescu,Dieter Printz,Gerhard Fritsch,Oswald Wagner,Edgar Selzer,Wolfgang R. Sperr,Peter Valent,Christian Sillaber +12 more
TL;DR: Overexpression of HO-1 in the CML-derived cell line K562 was found to counteract STI571-induced apoptosis and identifyHO-1 as a novel BCR/ABL-driven survival molecule and potential target in leukemic cells in patients with CML.
Journal ArticleDOI
The crucial role of multiomic approach in cancer research and clinically relevant outcomes
Miaolong Lu,Xianquan Zhan +1 more
TL;DR: The methodology, advances, and clinically relevant outcomes of different “omics” technologies in cancerResearch are described, and the importance and scientific merit of integrating multi-omics in cancer research and clinical relevant outcomes are emphasized.
Journal ArticleDOI
Altered transcription of an oncogene in chronic myeloid leukaemia
TL;DR: Leukaemic cells from 5 of 6 patients with chronic myelogenous leukaemia and the Ph1 chromosome were found to contain a new 8 kb abl RNA transcript, raising the possibility that the abl oncogene is directly involved in the development of CML.
Journal ArticleDOI
ADAR1 promotes malignant progenitor reprogramming in chronic myeloid leukemia
Qingfei Jiang,Leslie Crews,Christian L. Barrett,Hye Jung E. Chun,Angela C. Court,Jane Isquith,Jane Isquith,Maria A. Zipeto,Maria A. Zipeto,Daniel Goff,Mark D. Minden,Anil Sadarangani,Jessica M. Rusert,Kim Hien T. Dao,Sheldon R. Morris,Lawrence S.B. Goldstein,Marco A. Marra,Kelly A. Frazer,Catriona Jamieson +18 more
TL;DR: In this paper, whole-transcriptome sequencing of normal, chronic phase, and serially transplantable blast crisis chronic myeloid leukemia (CML) progenitors revealed increased IFNγ pathway gene expression in concert with BCR-ABL amplification, enhanced expression of the IFN-responsive ADAR1 p150 isoform, and a propensity for increased adenosine-to-inosine RNA editing during CML progression.
References
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Journal ArticleDOI
New Technique for Distinguishing between Human Chromosomes
TL;DR: It seems probable, therefore, that the darker staining with Giemsa of these regions, after denaturation and annealing, indicates the presence of highly repetitive DNA.
Journal ArticleDOI
Technique for Identifying Y Chromosomes in Human Interphase Nuclei
TL;DR: This work investigated the possibility of positively identifying male nuclei in interphase by virtue of this staining property of the Y chromosome using quinacrine dihydro-chloride.
Journal ArticleDOI
Clinical Implications of Cytogenetic Variants in Chronic Myelocytic Leukemia (CML)
TL;DR: The development of other chromosomal abnormalities in Ph1 positive patients presaged the terminal stage of the disease.
Journal ArticleDOI
Philadelphia-Chromosome-Positive and -Negative Chronic Myelocytic Leukemia
TL;DR: Chromosomal studies were performed on 61 adult patients with "typical chronic myelocytic leukemia" and the Philadelphia (Ph1) chromosome was found in 43 patients, with equal sex distribution a year after diagnosis.
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