Journal ArticleDOI
Letter: A new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by quinacrine fluorescence and Giemsa staining.
TLDR
An unsuspected abnormality in all cells from the nine patients with chronic myelogenous leukaemia has been detected with quinacrine fluorescence and various Giemsa staining techniques, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and thelong arm of 9, producing the 9q+ chromosome.Abstract:
CELLS from nine consecutive patients with chronic myelogenous leukaemia (CML) have been analysed with quinacrine fluorescence and various Giemsa staining techniques. The Philadelphia (Ph1) chromosome in all nine patients represents a deletion of the long arm of chromosome 22 (22q−)1,2. An unsuspected abnormality in all cells from the nine patients has been detected with these new staining techniques. It consists of the addition of dully fluorescing material to the end of the long arm of one chromosome 9 (9q+). In Giemsa-stained preparations, this material appears as an additional faint terminal band in one chromosome 9. The amount of additional material is approximately equal to the amount missing from the Ph1 (22q−) chromosome, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and the long arm of 9, producing the 9q+ chromosome.read more
Citations
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Journal ArticleDOI
The first intron in the human c-abl gene is at least 200 kilobases long and is a target for translocations in chronic myelogenous leukemia.
TL;DR: The c-abl protooncogene has multiple, widely space N-terminal coding exons transcribed by different promoters, and it is the target of the translocations that form the Philadelphia chromosome found in cells of chronic myelogenous leukemia patients.
Journal Article
Nonrandom Abnormalities in Chromosome 1 in Human Testicular Cancers
TL;DR: Trypsin G banding was performed on metaphase chromosomes from 14 cell lines derived from primary tumors or metastases of 11 patients with testicular cancer, finding consistent involvement of chromosome 1 in aberrations may be associated with the highly malignant nature of testicular cancers.
Journal ArticleDOI
The B cell mutator AID promotes B lymphoid blast crisis and drug-resistance in chronic myeloid leukemia
Lars Klemm,Lars Klemm,Lars Klemm,Cihangir Duy,Cihangir Duy,Cihangir Duy,Ilaria Iacobucci,Stefan Kuchen,Gregor von Levetzow,Gregor von Levetzow,Gregor von Levetzow,Niklas Feldhahn,Nadine Henke,Zhiyu Li,Thomas K. Hoffmann,Thomas K. Hoffmann,Yong-Mi Kim,Yong-Mi Kim,Wolf-Karsten Hofmann,Hassan Jumaa,John Groffen,John Groffen,Nora Heisterkamp,Nora Heisterkamp,Giovanni Martinelli,Michael R. Lieber,Rafael Casellas,Markus Müschen,Markus Müschen,Markus Müschen +29 more
TL;DR: It is demonstrated that AID expression in CML cells promotes overall genetic instability by hypermutation of tumor suppressor and DNA repair genes and provides a rationale for the rapid development of drug resistance and blast crisis progression.
Journal ArticleDOI
BCL6-mediated repression of p53 is critical for leukemia stem cell survival in chronic myeloid leukemia
Christian Hurtz,Christian Hurtz,Katerina Hatzi,Leandro Cerchietti,Melanie Braig,Eugene Park,Yong-Mi Kim,Sebastian Herzog,Parham Ramezani-Rad,Hassan Jumaa,Martin C. Müller,Wolf-Karsten Hofmann,Andreas Hochhaus,B. Hilda Ye,Anupriya Agarwal,Brian J. Druker,Neil P. Shah,Ari Melnick,Markus Müschen,Markus Müschen +19 more
TL;DR: Clinical validation of this concept could limit the duration of TKI treatment in CML patients, which is currently life-long, and substantially decrease the risk of blast crisis transformation, as well as suggest that pharmacological inhibition of BCL6 may represent a novel strategy to eradicate LICs in C ML.
Journal ArticleDOI
Adaptive secretion of granulocyte-macrophage colony-stimulating factor (GM-CSF) mediates imatinib and nilotinib resistance in BCR/ABL+ progenitors via JAK-2/STAT-5 pathway activation
Ying Wang,Dali Cai,Cornelia Brendel,Christine Barett,Philipp Erben,Paul W. Manley,Andreas Hochhaus,Andreas Neubauer,Andreas Burchert +8 more
TL;DR: Adaptive autocrine secretion of GM-CSF mediates BCR/ABL-independent IM and NI resistance via activation of the antiapoptotic Jak-2/STAT-5 pathway, providing a rationale for the application of JAK-2 inhibitors to eradicate residual disease in CML.
References
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Journal ArticleDOI
New Technique for Distinguishing between Human Chromosomes
TL;DR: It seems probable, therefore, that the darker staining with Giemsa of these regions, after denaturation and annealing, indicates the presence of highly repetitive DNA.
Journal ArticleDOI
Technique for Identifying Y Chromosomes in Human Interphase Nuclei
TL;DR: This work investigated the possibility of positively identifying male nuclei in interphase by virtue of this staining property of the Y chromosome using quinacrine dihydro-chloride.
Journal ArticleDOI
Clinical Implications of Cytogenetic Variants in Chronic Myelocytic Leukemia (CML)
TL;DR: The development of other chromosomal abnormalities in Ph1 positive patients presaged the terminal stage of the disease.
Journal ArticleDOI
Philadelphia-Chromosome-Positive and -Negative Chronic Myelocytic Leukemia
TL;DR: Chromosomal studies were performed on 61 adult patients with "typical chronic myelocytic leukemia" and the Philadelphia (Ph1) chromosome was found in 43 patients, with equal sex distribution a year after diagnosis.
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