Journal ArticleDOI
Letter: A new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by quinacrine fluorescence and Giemsa staining.
TLDR
An unsuspected abnormality in all cells from the nine patients with chronic myelogenous leukaemia has been detected with quinacrine fluorescence and various Giemsa staining techniques, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and thelong arm of 9, producing the 9q+ chromosome.Abstract:
CELLS from nine consecutive patients with chronic myelogenous leukaemia (CML) have been analysed with quinacrine fluorescence and various Giemsa staining techniques. The Philadelphia (Ph1) chromosome in all nine patients represents a deletion of the long arm of chromosome 22 (22q−)1,2. An unsuspected abnormality in all cells from the nine patients has been detected with these new staining techniques. It consists of the addition of dully fluorescing material to the end of the long arm of one chromosome 9 (9q+). In Giemsa-stained preparations, this material appears as an additional faint terminal band in one chromosome 9. The amount of additional material is approximately equal to the amount missing from the Ph1 (22q−) chromosome, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and the long arm of 9, producing the 9q+ chromosome.read more
Citations
More filters
Journal ArticleDOI
The specificity of chromosome A2 involvement in DMBA-induced rat sarcomas.
TL;DR: By detailed structural analysis of the A2-containing marker chromosomes the segment of critical significance was tentatively located to certain bands in the middle region of A2.
Journal ArticleDOI
Acute dasatinib exposure commits Bcr-Abl-dependent cells to apoptosis.
Jennifer Snead,Thomas O'Hare,Thomas O'Hare,Lauren T. Adrian,Lauren T. Adrian,Christopher A. Eide,Christopher A. Eide,Thoralf Lange,Brian J. Druker,Brian J. Druker,Michael W. Deininger,Michael W. Deininger +11 more
TL;DR: It is demonstrated that in vitro assays designed to model in vivo pharmacokinetics can predict clinical efficacy and challenge the widely held notion that continuous target inhibition is required for optimal efficacy of kinase inhibitors.
Journal ArticleDOI
Minimal residual disease in patients with chronic myelogenous leukemia undergoing long-term treatment with recombinant interferon alpha-2b alone or in combination with interferon gamma
Bertram Opalka,U. B. Wandl,Reinhard Becher,Otto Kloke,Marianne Nagel-Hiemke,Thomas Moritz,Ulrike Beer,Siegfried Seeber,Norbert Niederle +8 more
TL;DR: In 11 of 115 patients reported here, a complete loss of rearranged BCR fragments was observed in Southern blots of peripheral blood (PB) and/or bone marrow (BM) cell samples, and the reappearance of the malignant marker was not accompanied by a clinical relapse.
Journal ArticleDOI
Blocking cytokine signaling along with intense Bcr-Abl kinase inhibition induces apoptosis in primary CML progenitors
Devendra K Hiwase,Deborah L. White,Deborah L. White,Jason A. Powell,Verity A Saunders,Stephanie Zrim,Amity Frede,Mark A. Guthridge,Mark A. Guthridge,Angel F. Lopez,Angel F. Lopez,Richard J D'Andrea,Richard J D'Andrea,L B To,L B To,Junia V. Melo,Junia V. Melo,Sharad Kumar,Sharad Kumar,Timothy P. Hughes,Timothy P. Hughes +20 more
TL;DR: The contention that therapeutic strategies combining intense Bcr-Abl kinase inhibition and blockade of cytokine signaling pathways can be effective for eradication of CML progenitors is supported.
Journal ArticleDOI
What are we learning from the cancer genome
TL;DR: New features of cancer genomes that are being revealed by large-scale applications of massively parallel approaches to nucleic acid sequencing are reviewed, highlighting the formidable genetic heterogeneity within given cancer types that is appreciable with higher resolution profiling and larger sample sets.
References
More filters
Journal ArticleDOI
New Technique for Distinguishing between Human Chromosomes
TL;DR: It seems probable, therefore, that the darker staining with Giemsa of these regions, after denaturation and annealing, indicates the presence of highly repetitive DNA.
Journal ArticleDOI
Technique for Identifying Y Chromosomes in Human Interphase Nuclei
TL;DR: This work investigated the possibility of positively identifying male nuclei in interphase by virtue of this staining property of the Y chromosome using quinacrine dihydro-chloride.
Journal ArticleDOI
Clinical Implications of Cytogenetic Variants in Chronic Myelocytic Leukemia (CML)
TL;DR: The development of other chromosomal abnormalities in Ph1 positive patients presaged the terminal stage of the disease.
Journal ArticleDOI
Philadelphia-Chromosome-Positive and -Negative Chronic Myelocytic Leukemia
TL;DR: Chromosomal studies were performed on 61 adult patients with "typical chronic myelocytic leukemia" and the Philadelphia (Ph1) chromosome was found in 43 patients, with equal sex distribution a year after diagnosis.
Related Papers (5)
Induction of chronic myelogenous leukemia in mice by the P210bcr/abl gene of the Philadelphia chromosome
Effects of a selective inhibitor of the Abl tyrosine kinase on the growth of Bcr-Abl positive cells.
Imatinib compared with interferon and low-dose cytarabine for newly diagnosed chronic-phase chronic myeloid leukemia
Stephen G. O'Brien,François Guilhot,Richard A. Larson,Insa Gathmann,Michele Baccarani,Francisco Cervantes,Jan J. Cornelissen,Thomas Fischer,Andreas Hochhaus,Timothy P. Hughes,Klaus Lechner,Johan Lanng Nielsen,Philippe Rousselot,Josy Reiffers,Giuseppe Saglio,John D. Shepherd,Bengt Simonsson,Alois Gratwohl,John M. Goldman,Hagop M. Kantarjian,Kerry Taylor,Gregor Verhoef,Ann E. Bolton,Renaud Capdeville,Brian J. Druker +24 more