Agriculture and Agri-Food Canada

About: Agriculture and Agri-Food Canada is a facility organization based out in Ottawa, Ontario, Canada. It is known for research contribution in the topics: Population & Soil water. The organization has 10921 authors who have published 21332 publications receiving 748193 citations. The organization is also known as: Department of Agriculture and Agri-Food.

pORE: a modular binary vector series suited for both monocot and dicot plant transformation.

Abstract: We present a series of 14 binary vectors suitable for Agrobacterium-mediated transformation of dicotyledonous plants and adaptable for biolistic transformation of monocotyledonous plants. The vector size has been minimized by eliminating all non-essential elements from the vector backbone and T-DNA regions while maintaining the ability to replicate independently. The smallest of the vector series is 6.3 kb and possesses an extensive multiple cloning site with 21 unique restriction endonuclease sites that are compatible with common cloning, protein expression, yeast two-hybrid and other binary vectors. The T-DNA region was engineered using a synthetic designer oligonucleotide resulting in an entirely modular system whereby any vector element can be independently exchanged. The high copy number ColE1 origin of replication has been included to enhance plasmid yield in Escherichia coli. FRT recombination sites flank the selectable marker cassette regions and allow for in planta excision by FLP recombinase. The pORE series consists of three basic types; an ‘open’ set for general plant transformation, a ‘reporter’ set for promoter analysis and an ‘expression’ set for constitutive expression of transgenes. The sets comprise various combinations of promoters (P HPL, P ENTCUP2 and P TAPADH), selectable markers (nptII and pat) and reporter genes (gusA and smgfp).

Evaluation of the photocatalytic antimicrobial effects of a TiO2 nanocomposite food packaging film by in vitro and in vivo tests

Abstract: The ability to inactivate Pseudomonas spp. and Rhodotorula mucilaginosa by a photocatalyst thin film, obtained via a combination of anatase and rutile titanium dioxide incorporation into a low density polyethylene (LDPE) polymeric matrix by a melt blending method, was addressed in this study. The number of surviving cell of the Pseudomonas spp. was decreased by 4 log CFU/ml and 1.35 log CFU/ml after 3 h of UVA illumination on TiO2 nanocomposite thin film and LDPE thin film, respectively. The number of cells of R. mucilaginosa decreased by 2 log CFU/ml and 0.64 log CFU/ml on TiO2 nanocomposite thin film and LDPE thin film, respectively. In an in vivo test carried out on fresh pears packaged in TiO2 nanocomposite film and stored under illumination by a fluorescent light lamp at 5 °C for 17 days, the number of mesophilic bacteria and yeast cells decreased significantly compared to samples stored in LDPE film. The greatest effects were recorded by combining UVA illumination and active film. It was also proven that the photocatalyst thin film prepared by extrusion could be effectively used in fruit packaging applications.