Agriculture and Agri-Food Canada

About: Agriculture and Agri-Food Canada is a facility organization based out in Ottawa, Ontario, Canada. It is known for research contribution in the topics: Population & Soil water. The organization has 10921 authors who have published 21332 publications receiving 748193 citations. The organization is also known as: Department of Agriculture and Agri-Food.

Allatostatins: a growing family of neuropeptides with structural and functional diversity.

Abstract: The high degree of conservation of the core sequence of the "cockroach-types" of AST and their widespread distribution suggest that they should be considered a ubiquitous family of peptides within the invertebrates, regulating a range of important physiological processes. These functional processes, by either neural or humoral routes of action, include the inhibition of endocrine function, interneuronal functions, neuromodulatory roles, myotropic and myoendocrine roles, and direct action on biosynthetic pathways. The myomodulatory function appears to be conserved through evolutionary time, whereas the JH inhibitory activity appears to be confined to specific orders. This suggests that the myomodulatory role was the more ancestral of these two particular functions. Certainly, further purification and gene cloning as a means to precursor identification and functional analysis will be a prerequisite to understanding the diverse functions of this peptide family.

Observed soil temperature trends associated with climate change in Canada

Abstract: [1] Trends in soil temperature are important, but rarely reported, indicators of climate change. On the basis of the soil temperature data from 30 climate stations across Canada during 1958–2008, trends in soil temperatures at 5, 10, 20, 50, 100, and 150 cm depths were analyzed, together with atmospheric variables, such as air temperature, precipitation, and depth of snow on the ground, observed at the same locations. There was a significant positive trend with soil temperatures in spring and summer means, but not for the winter and annual means. A positive trend with time in soil temperature was detected at about two-thirds of the stations at all depths below 5 cm. A warming trend of 0.26–0.30°C/decade was consistently detected in spring (March–April–May) at all depths between 1958 and 2008. The warming trend in soil temperatures was associated with trends in air temperatures and snow cover depth over the same period. A significant decreasing trend in snow cover depth in winter and spring was associated with increasing air temperatures. The combined effects of the higher air temperature and reduced snow depth probably resulted in an enhanced increasing trend in spring soil temperatures, but no significant trends in winter soil temperatures. The thermal insulation by snow cover appeared to play an important role in the response of soil temperatures to climate change and must be accounted for in projecting future soil-related impacts of climate change.

Comparative structural, emulsifying, and biological properties of 2 major canola proteins, cruciferin and napin.

Abstract: Canola is an economically important farm-gate crop in Canada. To further explore the potential of canola protein as value-added food and nutraceutical ingredients, a better understanding of fundamental properties of 2 major canola proteins is necessary. Two major protein components, cruciferin and napin, were isolated from defatted canola meal by Sephacryl S-300 gel filtration chromatography. SDS-PAGE showed that cruciferin consists of more than 10 polypeptides, and noncovalent links are more important than disulphide bonds in stabilizing the structural conformation. Napin consists of 2 polypeptides and is stabilized primarily by disulphide bonds. Purified cruciferin showed 1 major endothermic peak at 91 degrees C compared with that of 110 degrees C for napin. Emulsion prepared by cruciferin showed significant higher specific surface area and lower particle size than that of napin. The study indicated that the presence of napin could detrimentally affect the emulsion stability of canola protein isolates. Hydrolysates from cruciferin and napin showed potent angiotensin I-converting enzyme inhibitory activity (IC(50): 0.035 and 0.029 mg/mL, respectively), but weaker than that of canola protein isolate hydrolysate (IC(50): 0.015 mg/mL).

Effects of tillage method and crop rotation on non-renewable energy use efficiency for a thin Black Chernozem in the Canadian Prairies.

Abstract: Producers in the Canadian Prairies have begun to extend and diversify their cereal-based rotations by including oilseed and pulse crops, and by managing these newer cropping systems with minimum- and zero-tillage practices. This study examined the implications of these land use changes on non-renewable energy requirements (both direct and indirect), energy output, and energy use efficiency for monoculture cereal, cereal–oilseed, and cereal–oilseed–pulse rotations, each managed using conventional (CT), minimum (MT), and zero (ZT) tillage practices on a thin Black Chernozem in Saskatchewan, Canada. The crop rotations included: spring wheat (Triticum aestivum L.)–spring wheat–winter wheat–fallow (Ws–Ws–Ww–F), spring wheat–spring wheat–flax (Linum usitatissimum L.)–winter wheat (Ws–Ws–Fx–Ww), and spring wheat–flax–winter wheat–field pea (Pisum sativum L.) (Ws–Fx–Ww–P). The findings, based on 12 years of data, showed that non-renewable energy use for the complete cropping systems was largely unaffected by tillage method, but that it differed significantly with crop rotations. Energy requirements were lowest for Ws–Ws–Ww–F (average 6389 MJ ha−1), intermediate for Ws–Fx–Ww–P (11% more), and highest for the Ws–Ws–Fx–Ww (28% more). The substitution of pea for spring wheat in the Ws–Fx–Ww–P versus Ws–Ws–Fx–Ww rotation reduced total energy use by 13%, reflecting the minimal requirement for N fertilizer by pulses due to their ability to biologically fix N, and from the lower fertilizer N rate that was applied to spring wheat grown after the legume. The use of MT and ZT practices provided significant energy savings (compared to CT) in on-farm use of fuel and in machine operation and manufacture for some cropping system components (e.g., summerfallow preparation, spring wheat grown on pea stubble, and for pea grown on cereal stubble), but these savings were often offset by higher energy requirements for herbicides and for N fertilizer with conservation tillage management. Gross energy output averaged 32 315 MJ ha−1 for Ws–Ws–Ww–F, 41 287 MJ ha−1 (or 28% more) for Ws–Ws–Fx–Ww, and 42 961 (or 33% more) for Ws–Fx–Ww–P. Tillage method had little overall influence on energy output for the monoculture cereal and cereal–oilseed–pulse rotations, but it was generally lower with CT than with MT or ZT management for the cereal–oilseed rotation. Energy use efficiency, measured as grain produced per unit of energy input and as the ratio of energy output to energy input, was highest for the cereal–oilseed–pulse rotation (373 and 6.1 kg GJ−1, respectively) and lower, but generally similar, for the cereal–oilseed and monoculture cereal rotations (298 and 5.1 kg GJ−1, respectively). The use of conservation tillage management enhanced overall energy use efficiency for the two mixed rotations, but not for the monoculture cereal rotation. We concluded that adopting diversified crop rotations, together with minimum and zero tillage management practices, will enhance non-renewable energy use efficiency of annual grain production in this sub-humid region.

Efficacy and Pharmacokinetics of Bacteriophage Therapy in Treatment of Subclinical Staphylococcus aureus Mastitis in Lactating Dairy Cattle

Abstract: Bovine mastitis is an inflammation of the udder caused by microbial infection. Mastitis caused by Staphylococcus aureus is a major concern to the dairy industry due to its resistance to antibiotic treatment and its propensity to recur chronically. Growing concerns surrounding antibiotic resistance have spurred research into alternative treatment methods. The ability of lytic S. aureus bacteriophage K to eliminate bovine S. aureus intramammary infection during lactation was evaluated in a placebo-controlled, multisite trial. Twenty-four lactating Holstein cows with preexisting subclinical S. aureus mastitis were treated. Treatment consisted of 10-ml intramammary infusions of either 1.25 x 10(11) PFU of phage K or saline, administered once per day for 5 days. The cure rate was established by the assessment of four serial samples collected following treatment. The cure rate was 3 of 18 quarters (16.7%) in the phage-treated group, while none of the 20 saline-treated quarters were cured. This difference was not statistically significant. The effects of phage intramammary infusion on the bovine mammary gland were also studied. In healthy lactating cows, a single infusion of either filter-sterilized broth lysate or a CsCl gradient-purified phage preparation elicited a large increase in the milk somatic cell count. This response was not observed when phage was infused into quarters which were already infected with S. aureus. Phage-infused healthy quarters continued to shed viable bacteriophage into the milk for up to 36 h postinfusion. The phage concentration in the milk suggested that there was significant degradation or inactivation of the infused phage within the gland.