Institution
Institute for Systems Biology
Nonprofit•Seattle, Washington, United States•
About: Institute for Systems Biology is a nonprofit organization based out in Seattle, Washington, United States. It is known for research contribution in the topics: Population & Proteomics. The organization has 1277 authors who have published 2777 publications receiving 353165 citations.
Topics: Population, Proteomics, Gene, Proteome, Systems biology
Papers published on a yearly basis
Papers
More filters
••
Fred Hutchinson Cancer Research Center1, University of Washington2, Icahn School of Medicine at Mount Sinai3, Canadian Institute for Advanced Research4, Cincinnati Children's Hospital Medical Center5, Institute for Systems Biology6, University of Toronto7, Baylor College of Medicine8, Sungkyunkwan University9, Cleveland Clinic Lerner Research Institute10, University of Edinburgh11
TL;DR: In vitro and in vivo validation of GSC-specific targets revealed several strong hits, including the wee1-like kinase, PKMYT1/Myt1, which acts redundantly with WEE1 to inhibit cyclin B-CDK1 activity via CDK1-Y15 phosphorylation and to promote timely completion of mitosis in NSCs.
148 citations
••
TL;DR: This study used ICAT technology and tandem mass spectrometry-based proteomics to systematically study protein expression in chronic pancreatitis and validated that cathepsin D, integrin β1, and plasminogen were overexpressed in both pancreatic cancer and chronic pancitis.
147 citations
••
TL;DR: This work used chemical labeling to isolate surface-exposed proteins on sporozoites and identified these proteins by mass spectrometry, providing the first direct evidence that the Plasmodium surface proteins CSP and TRAP are glycosylated in sporzoites, a finding that could impact the selection of vaccine antigens.
Abstract: Malaria parasite infection is initiated by the mosquito-transmitted sporozoite stage, a highly motile invasive cell that targets hepatocytes in the liver for infection. A promising approach to developing a malaria vaccine is the use of proteins located on the sporozoite surface as antigens to elicit humoral immune responses that prevent the establishment of infection. Very little of the P. falciparum genome has been considered as potential vaccine targets, and candidate vaccines have been almost exclusively based on single antigens, generating the need for novel target identification. The most advanced malaria vaccine to date, RTS,S, a subunit vaccine consisting of a portion of the major surface protein circumsporozoite protein (CSP), conferred limited protection in Phase III trials, falling short of community-established vaccine efficacy goals. In striking contrast to the limited protection seen in current vaccine trials, sterilizing immunity can be achieved by immunization with radiation-attenuated sporozoites, suggesting that more potent protection may be achievable with a multivalent protein vaccine. Here, we provide the most comprehensive analysis to date of proteins located on the surface of or secreted by Plasmodium falciparum salivary gland sporozoites. We used chemical labeling to isolate surface-exposed proteins on sporozoites and identified these proteins by mass spectrometry. We validated several of these targets and also provide evidence that components of the inner membrane complex are in fact surface-exposed and accessible to antibodies in live sporozoites. Finally, our mass spectrometry data provide the first direct evidence that the Plasmodium surface proteins CSP and TRAP are glycosylated in sporozoites, a finding that could impact the selection of vaccine antigens.
147 citations
••
University Medical Center Groningen1, Karolinska Institutet2, University of Paris3, Utrecht University4, National Health Service5, University of Crete6, University of Southern California7, Maastricht University8, University of Valencia9, University of Bologna10, University of Oslo11, Oslo University Hospital12, University of Helsinki13, McGill University14, University of Cambridge15, Institute for Systems Biology16, Boston Children's Hospital17, Swiss Tropical and Public Health Institute18, University of Basel19, University of Arizona20, Pompeu Fabra University21, University of Tampere22, French Institute of Health and Medical Research23, Radboud University Nijmegen24, National Institutes of Health25, University of Montpellier26, Charité27, University of Eastern Finland28, Université du Québec à Chicoutimi29, University of the Basque Country30, King's College London31, Stockholm County Council32
TL;DR: In this paper, a large-scale epigenome-wide association study (EWAS) within the Mechanisms of the Development of ALLergy (MeDALL) project was conducted to assess methylation profiles associated with childhood asthma.
147 citations
••
University of Montpellier1, French Institute of Health and Medical Research2, Swiss Institute of Allergy and Asthma Research3, Institute for Systems Biology4, Charité5, Paris Descartes University6, University Medical Center Groningen7, Medical University of Vienna8, Karolinska Institutet9, University of Toulouse10, University of Helsinki11, Ghent University12, Oslo University Hospital13, Pierre-and-Marie-Curie University14, Odense University Hospital15, Medical University of Łódź16, Biomax Informatics AG17, Utrecht University18, Bradford Royal Infirmary19, Karolinska University Hospital20, King's College London21, Ghent University Hospital22, University of Valencia23, University of Crete24, University of Bologna25, University of Paris26, Ludwig Maximilian University of Munich27, Helmholtz Centre for Environmental Research - UFZ28, Pompeu Fabra University29
TL;DR: The translational component of MeDALL is shown by the identification of a novel allergic phenotype characterized by polysensitization and multimorbidity, which is associated with the frequency, persistence, and severity of allergic symptoms.
Abstract: Asthma, rhinitis, and eczema are complex diseases with multiple genetic and environmental factors interlinked through IgE-associated and non-IgE-associated mechanisms. Mechanisms of the Development of ALLergy (MeDALL; EU FP7-CP-IP; project no: 261357; 2010-2015) studied the complex links of allergic diseases at the clinical and mechanistic levels by linking epidemiologic, clinical, and mechanistic research, including in vivo and in vitro models. MeDALL integrated 14 European birth cohorts, including 44,010 participants and 160 cohort follow-ups between pregnancy and age 20 years. Thirteen thousand children were prospectively followed after puberty by using a newly standardized MeDALL Core Questionnaire. A microarray developed for allergen molecules with increased IgE sensitivity was obtained for 3,292 children. Estimates of air pollution exposure from previous studies were available for 10,000 children. Omics data included those from historical genome-wide association studies (23,000 children) and DNA methylation (2,173), targeted multiplex biomarker (1,427), and transcriptomic (723) studies. Using classical epidemiology and machine-learning methods in 16,147 children aged 4 years and 11,080 children aged 8 years, MeDALL showed the multimorbidity of eczema, rhinitis, and asthma and estimated that only 38% of multimorbidity was attributable to IgE sensitization. MeDALL has proposed a new vision of multimorbidity independent of IgE sensitization, and has shown that monosensitization and polysensitization represent 2 distinct phenotypes. The translational component of MeDALL is shown by the identification of a novel allergic phenotype characterized by polysensitization and multimorbidity, which is associated with the frequency, persistence, and severity of allergic symptoms. The results of MeDALL will help integrate personalized, predictive, preventative, and participatory approaches in allergic diseases.
146 citations
Authors
Showing all 1292 results
Name | H-index | Papers | Citations |
---|---|---|---|
Younan Xia | 216 | 943 | 175757 |
Ruedi Aebersold | 182 | 879 | 141881 |
David Haussler | 172 | 488 | 224960 |
Steven P. Gygi | 172 | 704 | 129173 |
Nahum Sonenberg | 167 | 647 | 104053 |
Leroy Hood | 158 | 853 | 128452 |
Mark H. Ellisman | 117 | 637 | 55289 |
Wei Zhang | 112 | 1189 | 93641 |
John Ralph | 109 | 442 | 39238 |
Eric H. Davidson | 106 | 454 | 47058 |
James R. Heath | 103 | 425 | 58548 |
Alan Aderem | 99 | 246 | 46682 |
Anne-Claude Gingras | 97 | 336 | 40714 |
Trey Ideker | 97 | 306 | 72276 |
Michael H. Gelb | 94 | 506 | 34714 |