A multiregional proteomic survey of the postnatal human brain.
Becky C. Carlyle,Robert R. Kitchen,Jean Kanyo,Edward Z. Voss,Mihovil Pletikos,André M. M. Sousa,TuKiet T. Lam,Mark Gerstein,Nenad Sestan,Angus C. Nairn +9 more
TLDR
An in-depth proteomic survey of regions of the postnatal human brain, ranging in age from early infancy to adulthood, revealed varied patterns of protein–RNA relationships, with generally increased magnitudes of protein abundance differences between brain regions compared to RNA.Abstract:
Detailed observations of transcriptional, translational and post-translational events in the human brain are essential to improving our understanding of its development, function and vulnerability to disease. Here, we exploited label-free quantitative tandem mass-spectrometry to create an in-depth proteomic survey of regions of the postnatal human brain, ranging in age from early infancy to adulthood. Integration of protein data with existing matched whole-transcriptome sequencing (RNA-seq) from the BrainSpan project revealed varied patterns of protein-RNA relationships, with generally increased magnitudes of protein abundance differences between brain regions compared to RNA. Many of the differences amplified in protein data were reflective of cytoarchitectural and functional variation between brain regions. Comparing structurally similar cortical regions revealed significant differences in the abundances of receptor-associated and resident plasma membrane proteins that were not readily observed in the RNA expression data.read more
Citations
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Microglial regional heterogeneity and its role in the brain.
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Child Abuse, Depression, and Methylation in Genes Involved With Stress, Neural Plasticity, and Brain Circuitry
TL;DR: This study suggests that epigenetic changes in ID3, GRIN1, and TPPP genes, in combination with experiences of maltreatment, may confer risk for depression in children.
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Effects of Neurotrophic Factors in Glial Cells in the Central Nervous System: Expression and Properties in Neurodegeneration and Injury
TL;DR: This review focuses on the relationship between glia and NTFs including neurotrophins, GDNF-family ligands, CNTF family, and CDNF/MANF-family proteins as well as existing data on the glial phenotypes of NTF knockout mice and their effects on glia in disease models such as AD, PD, stroke, and retinal degeneration.
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Proteome profiling in cerebrospinal fluid reveals novel biomarkers of Alzheimer's disease
Jakob M. Bader,Philipp E. Geyer,Philipp E. Geyer,Johannes Müller,Maximilian T. Strauss,Manja Koch,Frank Leypoldt,Peter Koertvelyessy,Peter Koertvelyessy,Daniel Bittner,Carola G. Schipke,Enise I. Incesoy,Oliver Peters,Oliver Peters,Nikolaus Deigendesch,Mikael Simons,Majken K. Jensen,Majken K. Jensen,Henrik Zetterberg,Matthias Mann,Matthias Mann +20 more
TL;DR: A highly reproducible mass spectrometry (MS)‐based proteomics workflow for the in‐depth analysis of CSF from minimal sample amounts is presented and a consistent glycolytic signature across cohorts and a recent study suggests clinical utility of this proteomic signature.
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Cell-Surface Proteomic Profiling in the Fly Brain Uncovers Wiring Regulators
Jiefu Li,Shuo Han,Hongjie Li,Namrata D. Udeshi,Tanya Svinkina,D. R. Mani,Chuanyun Xu,Ricardo Guajardo,Qijing Xie,Tongchao Li,David J. Luginbuhl,Bing Wu,Colleen N McLaughlin,Anthony Xie,Pornchai Kaewsapsak,Stephen R. Quake,Steven A. Carr,Alice Y. Ting,Liqun Luo +18 more
TL;DR: A cell-type-specific, spatiotemporally resolved approach to profile cell-surface proteomes in intact tissues and revealed that the lipoprotein receptor LRP1 cell-autonomously controls PN dendrite targeting, contributing to the formation of a precise olfactory map.
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