Journal ArticleDOI
Derivation of embryonic stem-cell lines from human blastocysts.
Chad A. Cowan,Irinha Klimanskaya,Jill A. McMahon,Jocelyn Atienza,Jeannine Witmyer,Jacob P. Zucker,Shunping Wang,Cynthia C. Morton,Andrew P. McMahon,Doug Powers,Douglas A. Melton +10 more
TLDR
The procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts are discussed.Abstract:
This report, first published online on March 3, 2004, discusses the procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts. These cell lines are available to researchers under a Material Transfer Agreement; according to current regulations, the cells cannot be used for research supported by federal funds. These cells are expected to facilitate research on a variety of serious chronic diseases.read more
Citations
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A human neuron injury model for molecular studies of axonal regeneration.
Lina Ziegler,Yael Segal-Ruder,Giovanni Coppola,Arbel Reis,Daniel H. Geschwind,Mike Fainzilber,Ronald S. Goldstein +6 more
TL;DR: Human embryonic stem cell-derived neurons may be a useful in vitro model system for mechanistic studies on human axonal injury and regeneration, and expression of several genes reported to be involved in axonal Injury responses in animal models changed following injury of hESC- derived neurons.
Journal ArticleDOI
Laser-Based Propagation of Human iPS and ES Cells Generates Reproducible Cultures with Enhanced Differentiation Potential
Kristi A. Hohenstein Elliott,Cory Peterson,Anuradha Soundararajan,Natalia Kan,Brandon Nelson,Sean Spiering,Mark Mercola,Gary R. Bright +7 more
TL;DR: A novel approach for the efficient, consistent expansion of human ESCs and iPSCs using laser sectioning, instead of mechanical devices or enzymes, to divide cultures into defined size clumps for propagation, maintained the pluripotency, quality, and genetic stability of ESCs/iPSCs and led to enhanced differentiation potential.
Journal ArticleDOI
A comparative transcriptomic study on the effects of valproic acid on two different hESCs lines in a neural teratogenicity test system
Silvia Colleoni,Cesare Galli,John Antonydas Gaspar,Kesavan Meganathan,Smita Jagtap,Jürgen Hescheler,Dimitra Zagoura,Susanne Bremer,Agapios Sachinidis,Giovanna Lazzari +9 more
TL;DR: This retrospective study compares microarray transcriptomic data derived from two different hESCs lines exposed to valproic acid while applying the same differentiation protocol and shows molecular changes in the processes of neural development, neural crest migration, apoptosis and regulation of transcription, indicating a good correspondence with the available in vivo data.
Journal ArticleDOI
Islet transplantation: current status and future directions.
TL;DR: The post-Edmonton era, the selection of islet transplant recipients, the production ofIslet grafts, and the need for immunosuppression and procedure-related risks are reviewed.
Journal ArticleDOI
High-resolution analysis of the subtelomeric regions of human embryonic stem cells.
Catarina Darnfors,Anna Flodin,Katarina Andersson,Gunilla Caisander,Jenny Lindqvist,Johan Hyllner,Jan Wahlström,Peter Sartipy +7 more
TL;DR: It is shown that no subtelomeric anomalies were detected in any of the nine hESC lines investigated, supporting the conclusion that hESCs, under appropriate conditions, maintain genomic stability during in vitro propagation.
References
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Journal ArticleDOI
Embryonic Stem Cell Lines Derived from Human Blastocysts
James A. Thomson,Joseph Itskovitz-Eldor,Sander S. Shapiro,Michelle A. Waknitz,Swiergiel Jennifer J,Vivienne S. Marshall,Jeffrey M. Jones +6 more
TL;DR: Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages.
Journal ArticleDOI
The serial cultivation of human diploid cell strains.
Leonard Hayflick,P.S. Moorhead +1 more
TL;DR: A consideration of the cause of the eventual degeneration of these strains leads to the hypothesis that non-cumulative external factors are excluded and that the phenomenon is attributable to intrinsic factors which are expressed as senescence at the cellular level.
Journal ArticleDOI
Formation of Pluripotent Stem Cells in the Mammalian Embryo Depends on the POU Transcription Factor Oct4
Jennifer Nichols,Branko Zevnik,Konstantinos Anastassiadis,Hitoshi Niwa,Daniela Klewe-Nebenius,Ian Chambers,Hans R. Schöler,Austin Smith +7 more
TL;DR: It is reported that the activity of Oct4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo and also determines paracrine growth factor signaling from stem cells to the trophectoderm.
Journal ArticleDOI
Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro.
TL;DR: The derivation of pluripotent embryonic stem (ES) cells from human blastocysts is described, providing a model to study early human embryology, an investigational tool for discovery of novel growth factors and medicines, and a potential source of cells for use in transplantation therapy.
Journal ArticleDOI
Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers.
Joseph Itskovitz-Eldor,Maya Schuldiner,Dorit Karsenti,Amir Eden,Ofra Yanuka,Michal Amit,Hermona Soreq,Nissim Benvenisty +7 more
TL;DR: The ability to induce formation of human embryoid bodies that contain cells of neuronal, hematopoietic and cardiac origins will be useful in studying early human embryonic development as well as in transplantation medicine.