Journal ArticleDOI
Derivation of embryonic stem-cell lines from human blastocysts.
Chad A. Cowan,Irinha Klimanskaya,Jill A. McMahon,Jocelyn Atienza,Jeannine Witmyer,Jacob P. Zucker,Shunping Wang,Cynthia C. Morton,Andrew P. McMahon,Doug Powers,Douglas A. Melton +10 more
TLDR
The procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts are discussed.Abstract:
This report, first published online on March 3, 2004, discusses the procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts. These cell lines are available to researchers under a Material Transfer Agreement; according to current regulations, the cells cannot be used for research supported by federal funds. These cells are expected to facilitate research on a variety of serious chronic diseases.read more
Citations
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Motivation to donate or not donate surplus embryos for stem-cell research: literature review.
TL;DR: The findings of studies regarding the factors influencing couples' decisions about whether or not to donate surplus embryos for research, including research purpose, treatment stage, embryo quality, religious beliefs, and altruism are presented.
Journal ArticleDOI
Progress and prospects: gene transfer into embryonic stem cells
TL;DR: The prospect of being able to select, isolate and expand a single cell to a vast population of cells could achieve a unique level of quality control, until now unattainable in the field of gene therapy.
Journal ArticleDOI
Neurodegeneration and cell replacement
TL;DR: The ultimate success of clinical stem cell-based strategies will depend on detailed understanding of stem cell biology in the degenerating brain and detailed evaluation of their functional efficacy and safety in preclinical animal models.
Journal ArticleDOI
High-efficiency derivation of human embryonic stem cell lines following pre-implantation genetic diagnosis.
Philippe Tropel,Johana Tournois,Julien Côme,Christine Varela,Céline Moutou,Pascal Fragner,Michel Cailleret,Yacine Laâbi,Marc Peschanski,Stéphane Viville +9 more
TL;DR: The results indicate that inner cell mass isolation is not mandatory for hESC derivation and that minimal manipulation of embryos can lead to high success rate.
Journal ArticleDOI
Biology of Isl1+ cardiac progenitor cells in development and disease.
TL;DR: In the postnatal heart, a novel cardiac cell type is identified, capable of self-renewal and readily differentiates into mature cardiomyocytes, which would complement in vitro studies in the mouse embryo during cardiac development.
References
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Journal ArticleDOI
Embryonic Stem Cell Lines Derived from Human Blastocysts
James A. Thomson,Joseph Itskovitz-Eldor,Sander S. Shapiro,Michelle A. Waknitz,Swiergiel Jennifer J,Vivienne S. Marshall,Jeffrey M. Jones +6 more
TL;DR: Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages.
Journal ArticleDOI
The serial cultivation of human diploid cell strains.
Leonard Hayflick,P.S. Moorhead +1 more
TL;DR: A consideration of the cause of the eventual degeneration of these strains leads to the hypothesis that non-cumulative external factors are excluded and that the phenomenon is attributable to intrinsic factors which are expressed as senescence at the cellular level.
Journal ArticleDOI
Formation of Pluripotent Stem Cells in the Mammalian Embryo Depends on the POU Transcription Factor Oct4
Jennifer Nichols,Branko Zevnik,Konstantinos Anastassiadis,Hitoshi Niwa,Daniela Klewe-Nebenius,Ian Chambers,Hans R. Schöler,Austin Smith +7 more
TL;DR: It is reported that the activity of Oct4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo and also determines paracrine growth factor signaling from stem cells to the trophectoderm.
Journal ArticleDOI
Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro.
TL;DR: The derivation of pluripotent embryonic stem (ES) cells from human blastocysts is described, providing a model to study early human embryology, an investigational tool for discovery of novel growth factors and medicines, and a potential source of cells for use in transplantation therapy.
Journal ArticleDOI
Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers.
Joseph Itskovitz-Eldor,Maya Schuldiner,Dorit Karsenti,Amir Eden,Ofra Yanuka,Michal Amit,Hermona Soreq,Nissim Benvenisty +7 more
TL;DR: The ability to induce formation of human embryoid bodies that contain cells of neuronal, hematopoietic and cardiac origins will be useful in studying early human embryonic development as well as in transplantation medicine.