Journal ArticleDOI
Derivation of embryonic stem-cell lines from human blastocysts.
Chad A. Cowan,Irinha Klimanskaya,Jill A. McMahon,Jocelyn Atienza,Jeannine Witmyer,Jacob P. Zucker,Shunping Wang,Cynthia C. Morton,Andrew P. McMahon,Doug Powers,Douglas A. Melton +10 more
TLDR
The procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts are discussed.Abstract:
This report, first published online on March 3, 2004, discusses the procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts. These cell lines are available to researchers under a Material Transfer Agreement; according to current regulations, the cells cannot be used for research supported by federal funds. These cells are expected to facilitate research on a variety of serious chronic diseases.read more
Citations
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Journal ArticleDOI
Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
Kazutoshi Takahashi,Koji Tanabe,Mari Ohnuki,Megumi Narita,Tomoko Ichisaka,Kiichiro Tomoda,Shinya Yamanaka +6 more
TL;DR: It is demonstrated that iPS cells can be generated from adult human fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc.
Journal ArticleDOI
Induction of Pluripotent Stem Cells From Adult Human Fibroblasts by Defined Factors
Kazutoshi Takahashi,Koji Tanabe,Mari Ohnuki,Megumi Narita,Tomoko Ichisaka,Kiichiro Tomoda,Shinya Yamanaka +6 more
TL;DR: This work generated induced pluripotent stem cells capable of germline transmission from murine somatic cells by transd, and demonstrated the ability of these cells to reprogram into patient-specific and disease-specific stem cells.
Journal ArticleDOI
Control of developmental regulators by Polycomb in human embryonic stem cells.
Tong Ihn Lee,Richard G. Jenner,Laurie A. Boyer,Matthew G. Guenther,Stuart S. Levine,Roshan M. Kumar,Brett Chevalier,Sarah E. Johnstone,Megan F. Cole,Kyoichi Isono,Haruhiko Koseki,Takuya Fuchikami,Kuniya Abe,Heather L. Murray,Jacob P. Zucker,Bingbing Yuan,George W. Bell,Elizabeth Herbolsheimer,Nancy M. Hannett,Kaiming Sun,Duncan T. Odom,Arie P. Otte,Thomas L. Volkert,David P. Bartel,Douglas A. Melton,David K. Gifford,David K. Gifford,Rudolf Jaenisch,Richard A. Young +28 more
TL;DR: It is found that PRC2 target genes are preferentially activated during ES cell differentiation and that the ES cell regulators OCT4, SOX2, and NANOG cooccupy a significant subset of these genes.
Journal ArticleDOI
Induced pluripotent stem cells generated from patients with ALS can be differentiated into motor neurons.
John T. Dimos,Kit T. Rodolfa,Kathy K. Niakan,Laurin M. Weisenthal,Hiroshi Mitsumoto,Wendy K. Chung,Wendy K. Chung,Gist F. Croft,Genevieve Saphier,Rudy Leibel,Robin Goland,Hynek Wichterle,Christopher E. Henderson,Kevin Eggan +13 more
TL;DR: Induced pluripotent stem cells are generated from an 82-year-old woman diagnosed with a familial form of amyotrophic lateral sclerosis and were successfully directed to differentiate into motor neurons, the cell type destroyed in ALS.
Journal ArticleDOI
A Chromatin Landmark and Transcription Initiation at Most Promoters in Human Cells
TL;DR: The results of a genome-wide analysis of human cells suggest that most protein-coding genes, including most genes thought to be transcriptionally inactive, experience transcription initiation, and that transcription initiation at most genes is a general phenomenon in human cells.
References
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Book ChapterDOI
41 – Approaches for Derivation and Maintenance of Human ES Cells: Detailed Procedures and Alternatives
TL;DR: In this paper, the authors describe several techniques and approaches which allowed them to derive over 30 hESC lines including derivation under feeder-free conditions and from a single blastomere.