Journal ArticleDOI
Derivation of embryonic stem-cell lines from human blastocysts.
Chad A. Cowan,Irinha Klimanskaya,Jill A. McMahon,Jocelyn Atienza,Jeannine Witmyer,Jacob P. Zucker,Shunping Wang,Cynthia C. Morton,Andrew P. McMahon,Doug Powers,Douglas A. Melton +10 more
TLDR
The procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts are discussed.Abstract:
This report, first published online on March 3, 2004, discusses the procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts. These cell lines are available to researchers under a Material Transfer Agreement; according to current regulations, the cells cannot be used for research supported by federal funds. These cells are expected to facilitate research on a variety of serious chronic diseases.read more
Citations
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Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
Kazutoshi Takahashi,Koji Tanabe,Mari Ohnuki,Megumi Narita,Tomoko Ichisaka,Kiichiro Tomoda,Shinya Yamanaka +6 more
TL;DR: It is demonstrated that iPS cells can be generated from adult human fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc.
Journal ArticleDOI
Induction of Pluripotent Stem Cells From Adult Human Fibroblasts by Defined Factors
Kazutoshi Takahashi,Koji Tanabe,Mari Ohnuki,Megumi Narita,Tomoko Ichisaka,Kiichiro Tomoda,Shinya Yamanaka +6 more
TL;DR: This work generated induced pluripotent stem cells capable of germline transmission from murine somatic cells by transd, and demonstrated the ability of these cells to reprogram into patient-specific and disease-specific stem cells.
Journal ArticleDOI
Control of developmental regulators by Polycomb in human embryonic stem cells.
Tong Ihn Lee,Richard G. Jenner,Laurie A. Boyer,Matthew G. Guenther,Stuart S. Levine,Roshan M. Kumar,Brett Chevalier,Sarah E. Johnstone,Megan F. Cole,Kyoichi Isono,Haruhiko Koseki,Takuya Fuchikami,Kuniya Abe,Heather L. Murray,Jacob P. Zucker,Bingbing Yuan,George W. Bell,Elizabeth Herbolsheimer,Nancy M. Hannett,Kaiming Sun,Duncan T. Odom,Arie P. Otte,Thomas L. Volkert,David P. Bartel,Douglas A. Melton,David K. Gifford,David K. Gifford,Rudolf Jaenisch,Richard A. Young +28 more
TL;DR: It is found that PRC2 target genes are preferentially activated during ES cell differentiation and that the ES cell regulators OCT4, SOX2, and NANOG cooccupy a significant subset of these genes.
Journal ArticleDOI
Induced pluripotent stem cells generated from patients with ALS can be differentiated into motor neurons.
John T. Dimos,Kit T. Rodolfa,Kathy K. Niakan,Laurin M. Weisenthal,Hiroshi Mitsumoto,Wendy K. Chung,Wendy K. Chung,Gist F. Croft,Genevieve Saphier,Rudy Leibel,Robin Goland,Hynek Wichterle,Christopher E. Henderson,Kevin Eggan +13 more
TL;DR: Induced pluripotent stem cells are generated from an 82-year-old woman diagnosed with a familial form of amyotrophic lateral sclerosis and were successfully directed to differentiate into motor neurons, the cell type destroyed in ALS.
Journal ArticleDOI
A Chromatin Landmark and Transcription Initiation at Most Promoters in Human Cells
TL;DR: The results of a genome-wide analysis of human cells suggest that most protein-coding genes, including most genes thought to be transcriptionally inactive, experience transcription initiation, and that transcription initiation at most genes is a general phenomenon in human cells.
References
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Journal ArticleDOI
The chromosome make-up of mouse embryonic stem cells is predictive of somatic and germ cell chimaerism
TL;DR: This study suggests that aneuploidy, rather than ‘loss of totipotency’, in ES cells, is the major cause of failure in obtaining contributions to all tissues of the adult chimaera, including the germline.
Journal Article
New insights into testicular germ cell tumorigenesis from gene expression profiling.
Rolf Inge Skotheim,Outi Monni,Spyro Mousses,Sophie D. Fosså,Olli Kallioniemi,Ragnhild A. Lothe,Anne Kallioniemi,Anne Kallioniemi +7 more
TL;DR: A focused functional genomic characterization of TGCTs has lead to the identification of new gene targets associated with a common genomic rearrangement as well as other genes with potential importance to testicular tumorigenesis.
Journal ArticleDOI
Male and female mice derived from the same embryonic stem cell clone by tetraploid embryo complementation.
Kevin Eggan,Anja Rode,Isabell Jentsch,Caroline Samuel,Thomas Hennek,Hartmut Tintrup,Branko Zevnik,Jennifer A. Erwin,Janet M. Loring,Laurie Jackson-Grusby,Michael R. Speicher,Ralf Kuehn,Rudolf Jaenisch +12 more
TL;DR: The time and expense required for production of experimental mutant animals from a targeted ES cell clone are substantially reduced and the data indicate that ES cells have inherently unstable karyotypes, but this instability does not interfere with production of adult ES cell–tetraploid mice.
Journal ArticleDOI
Molecular cytogenetic analysis of i(12p)-negative human male germ cell tumors.
Eduardo Rodriguez,Jane Houldsworth,Victor E. Reuter,Paul S. Meltzer,Ji Zhang,Jeffrey M. Trent,George J. Bosl,R. S. K. Chaganti +7 more
TL;DR: Both i(12p)‐negative and ‐positive groups are characterized by an increased copy number of 12p, which is consistent with a lack of significant clinical or biological difference between them.
Journal ArticleDOI
The rapid quantitation of serum alpha-fetoprotein by two-site micro enzyme immunoassay
D.J. Macdonald,Anne M. Kelly +1 more
TL;DR: A simple, inexpensive and rapid micro two-site enzyme immunoassay procedure for serum alpha-fetoprotein is described, which is eminently suitable for the screening of neural tube defects and for the quantitation of AFP as a tumour marker.