Journal ArticleDOI
Derivation of embryonic stem-cell lines from human blastocysts.
Chad A. Cowan,Irinha Klimanskaya,Jill A. McMahon,Jocelyn Atienza,Jeannine Witmyer,Jacob P. Zucker,Shunping Wang,Cynthia C. Morton,Andrew P. McMahon,Doug Powers,Douglas A. Melton +10 more
TLDR
The procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts are discussed.Abstract:
This report, first published online on March 3, 2004, discusses the procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts. These cell lines are available to researchers under a Material Transfer Agreement; according to current regulations, the cells cannot be used for research supported by federal funds. These cells are expected to facilitate research on a variety of serious chronic diseases.read more
Citations
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Isolation of amniotic stem cell lines with potential for therapy
Paolo De Coppi,Georg Bartsch,M. Minhaj Siddiqui,Tao Xu,Cesar C. Santos,Laura Perin,Gustavo Mostoslavsky,Ang line C. Serre,Evan Y. Snyder,James J. Yoo,Mark E. Furth,Shay Soker,Anthony Atala +12 more
TL;DR: It is affirm that stem cells capable of extensive self-renewal can routinely be obtained from human amniotic fluid and that AFS cells are pluripotent stem cells.
Journal ArticleDOI
Fibroblast Growth Factor 4 and Its Novel Splice Isoform Have Opposing Effects on the Maintenance of Human Embryonic Stem Cell Self‐Renewal
TL;DR: Results suggest a growth‐promoting role for FGF4 in HESCs and a putative feedback inhibition mechanism by a novel F GF4 splice isoform that may serve to promote differentiation at later stages of development.
Journal ArticleDOI
Differentially expressed, variant U1 snRNAs regulate gene expression in human cells.
Dawn O'Reilly,Martin Dienstbier,Sally A. Cowley,Pilar Vazquez,Marek M. Drozdz,Stephen S. Taylor,William James,Shona Murphy +7 more
TL;DR: This work has identified global transcriptome changes following interrogation of the Affymetrix Human Exon ST 1.0 array and indicates that this vU1 snRNA regulates expression of a subset of target genes at the level of pre-mRNA processing.
Journal ArticleDOI
Normal Human Pluripotent Stem Cell Lines Exhibit Pervasive Mosaic Aneuploidy
Suzanne E. Peterson,Jurjen W. Westra,Jurjen W. Westra,Stevens K. Rehen,Holly Young,Diane M. Bushman,Diane M. Bushman,Christine M. Paczkowski,Yun C. Yung,Yun C. Yung,Candace L. Lynch,Ha T. Tran,Kyle S. Nickey,Yu-Chieh Wang,Louise C. Laurent,Louise C. Laurent,Jeanne F. Loring,Melissa K. Carpenter,Jerold Chun +18 more
TL;DR: It is reported that normal hPSC – including induced pluripotent - lines are karyotypic mosaics of euploid cells intermixed with many cells showing non-clonal aneuploidies as identified by chromosome counting, spectral karyotypesing (SKY) and fluorescent in situ hybridization (FISH) of interphase/non-mitotic cells.
Journal ArticleDOI
MicroRNA-22 regulates smooth muscle cell differentiation from stem cells by targeting methyl CpG-binding protein 2.
Hanqing Zhao,Guanmei Wen,Yuan Huang,Xiaotian Yu,Qishan Chen,Tayyab Adeel Afzal,Le Anh Luong,Jianhua Zhu,Shu Ye,Li Zhang,Qingzhong Xiao +10 more
TL;DR: MiR-22 plays an important role in SMC differentiation, and epigenetic regulation through MECP2 is required for miR- 22 mediated SMC differentiated cells.
References
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Journal ArticleDOI
Embryonic Stem Cell Lines Derived from Human Blastocysts
James A. Thomson,Joseph Itskovitz-Eldor,Sander S. Shapiro,Michelle A. Waknitz,Swiergiel Jennifer J,Vivienne S. Marshall,Jeffrey M. Jones +6 more
TL;DR: Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages.
Journal ArticleDOI
The serial cultivation of human diploid cell strains.
Leonard Hayflick,P.S. Moorhead +1 more
TL;DR: A consideration of the cause of the eventual degeneration of these strains leads to the hypothesis that non-cumulative external factors are excluded and that the phenomenon is attributable to intrinsic factors which are expressed as senescence at the cellular level.
Journal ArticleDOI
Formation of Pluripotent Stem Cells in the Mammalian Embryo Depends on the POU Transcription Factor Oct4
Jennifer Nichols,Branko Zevnik,Konstantinos Anastassiadis,Hitoshi Niwa,Daniela Klewe-Nebenius,Ian Chambers,Hans R. Schöler,Austin Smith +7 more
TL;DR: It is reported that the activity of Oct4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo and also determines paracrine growth factor signaling from stem cells to the trophectoderm.
Journal ArticleDOI
Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro.
TL;DR: The derivation of pluripotent embryonic stem (ES) cells from human blastocysts is described, providing a model to study early human embryology, an investigational tool for discovery of novel growth factors and medicines, and a potential source of cells for use in transplantation therapy.
Journal ArticleDOI
Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers.
Joseph Itskovitz-Eldor,Maya Schuldiner,Dorit Karsenti,Amir Eden,Ofra Yanuka,Michal Amit,Hermona Soreq,Nissim Benvenisty +7 more
TL;DR: The ability to induce formation of human embryoid bodies that contain cells of neuronal, hematopoietic and cardiac origins will be useful in studying early human embryonic development as well as in transplantation medicine.