Journal ArticleDOI
Derivation of embryonic stem-cell lines from human blastocysts.
Chad A. Cowan,Irinha Klimanskaya,Jill A. McMahon,Jocelyn Atienza,Jeannine Witmyer,Jacob P. Zucker,Shunping Wang,Cynthia C. Morton,Andrew P. McMahon,Doug Powers,Douglas A. Melton +10 more
TLDR
The procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts are discussed.Abstract:
This report, first published online on March 3, 2004, discusses the procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts. These cell lines are available to researchers under a Material Transfer Agreement; according to current regulations, the cells cannot be used for research supported by federal funds. These cells are expected to facilitate research on a variety of serious chronic diseases.read more
Citations
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Lentiviral Vector Gene Transfer Is Limited by the Proteasome at Postentry Steps in Various Types of Stem Cells
Francesca Romana Santoni de Sio,Francesca Romana Santoni de Sio,Angela Gritti,Paolo Cascio,Margherita Neri,Maurilio Sampaolesi,Cesare Galli,Jeremy Luban,Luigi Naldini,Luigi Naldini +9 more
TL;DR: The proteasome limits lentiviral gene transfer in all stem cell types tested, including embryonic, mesenchymal, and neural, of both human and mouse origin, suggesting that it represents a novel feature of the stem cell/immature progenitor phenotype.
Journal ArticleDOI
Similar pattern in cardiac differentiation of human embryonic stem cell lines, BG01V and ReliCell®hES1, under low serum concentration supplemented with bone morphogenetic protein‐2
Rajarshi Pal,Aparna Khanna +1 more
TL;DR: The same differentiation conditions functioned in two independently derived hESC lines and may provide valuable insights for testing of other factors that might promote cardiomyocyte differentiation in low-serum formulations.
Journal ArticleDOI
An Improved Technique for Chromosomal Analysis of Human ES and iPS Cells
Daniela Moralli,Mohammed Yusuf,Mohammad A. Mandegar,Suhail Khoja,Zoia L. Monaco,Emanuela V. Volpi +5 more
TL;DR: This protocol represents a significant advancement in this line of work, and has the required attributes of simplicity and consistency to be widely accepted as a reference method for high quality, fast chromosome analysis of human ES and iPS cells.
Journal ArticleDOI
Brief report: benchmarking human pluripotent stem cell markers during differentiation into the three germ layers unveils a striking heterogeneity: all markers are not equal.
Jean-Marie Ramirez,Sabine Gerbal-Chaloin,Ollivier Milhavet,Bai Qiang,Fabienne Becker,Said Assou,Jean-Marc Lemaitre,Samir Hamamah,John De Vos +8 more
TL;DR: It is shown that differentiation markers are coexpressed with pluripotency markers before the latter begin to disappear, suggesting that OCT4, SSEA3, and TRA‐1‐60 might be better to trace in vitro the emergence of pluripotent cells during reprogramming.
Journal ArticleDOI
A multi-phenotypic cancer model with cell plasticity
TL;DR: It is proved that under very weak assumption, the nonlinear dynamics of multi-phenotypic proportions in the model has only one stable steady state and no stable limit cycle, which theoretically explains the phenotypic equilibrium phenomena reported in various cancer cell lines.
References
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Journal ArticleDOI
Embryonic Stem Cell Lines Derived from Human Blastocysts
James A. Thomson,Joseph Itskovitz-Eldor,Sander S. Shapiro,Michelle A. Waknitz,Swiergiel Jennifer J,Vivienne S. Marshall,Jeffrey M. Jones +6 more
TL;DR: Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages.
Journal ArticleDOI
The serial cultivation of human diploid cell strains.
Leonard Hayflick,P.S. Moorhead +1 more
TL;DR: A consideration of the cause of the eventual degeneration of these strains leads to the hypothesis that non-cumulative external factors are excluded and that the phenomenon is attributable to intrinsic factors which are expressed as senescence at the cellular level.
Journal ArticleDOI
Formation of Pluripotent Stem Cells in the Mammalian Embryo Depends on the POU Transcription Factor Oct4
Jennifer Nichols,Branko Zevnik,Konstantinos Anastassiadis,Hitoshi Niwa,Daniela Klewe-Nebenius,Ian Chambers,Hans R. Schöler,Austin Smith +7 more
TL;DR: It is reported that the activity of Oct4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo and also determines paracrine growth factor signaling from stem cells to the trophectoderm.
Journal ArticleDOI
Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro.
TL;DR: The derivation of pluripotent embryonic stem (ES) cells from human blastocysts is described, providing a model to study early human embryology, an investigational tool for discovery of novel growth factors and medicines, and a potential source of cells for use in transplantation therapy.
Journal ArticleDOI
Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers.
Joseph Itskovitz-Eldor,Maya Schuldiner,Dorit Karsenti,Amir Eden,Ofra Yanuka,Michal Amit,Hermona Soreq,Nissim Benvenisty +7 more
TL;DR: The ability to induce formation of human embryoid bodies that contain cells of neuronal, hematopoietic and cardiac origins will be useful in studying early human embryonic development as well as in transplantation medicine.