Journal ArticleDOI
Derivation of embryonic stem-cell lines from human blastocysts.
Chad A. Cowan,Irinha Klimanskaya,Jill A. McMahon,Jocelyn Atienza,Jeannine Witmyer,Jacob P. Zucker,Shunping Wang,Cynthia C. Morton,Andrew P. McMahon,Doug Powers,Douglas A. Melton +10 more
TLDR
The procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts are discussed.Abstract:
This report, first published online on March 3, 2004, discusses the procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts. These cell lines are available to researchers under a Material Transfer Agreement; according to current regulations, the cells cannot be used for research supported by federal funds. These cells are expected to facilitate research on a variety of serious chronic diseases.read more
Citations
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Journal ArticleDOI
Genetic approach to track neural cell fate decisions using human embryonic stem cells
Xuemei Fu,Xuemei Fu,Zhili Rong,Shengyun Zhu,Shengyun Zhu,Xiaocheng Wang,Yang Xu,Blue B. Lake +7 more
TL;DR: A fluorescent reporter system that can be used to trace neural differentiation events of hESCs and permits the identification of different neural subpopulations based on the intensity of the fluorescent reporter.
Patent
Method for generating primate cardiovascular progenitor cells for clinical use from primate embryonic stem cells or embryonic-like state cells, and their applications
TL;DR: In this paper, a method for the in vitro preparation of cardiovascular progenitors cells from mammalian embryonic stem cells (ES cells) or mammalian embryonic-like state cells, preferably from primate, was presented.
Journal ArticleDOI
Non-embryo-destructive Extraction of Pluripotent Embryonic Stem Cells: Implications for Regenerative Medicine and Reproductive Medicine.
TL;DR: In 2013, the German Patent and Trademark Office issued a patent for the non-embryo-destructive extraction of pluripotent embryonic stem cells and their uses as mentioned in this paper.
Journal ArticleDOI
Maturation of pluripotent stem cell derived cardiomyocytes: The new challenge
Idil Aigha,Christophe M. Raynaud +1 more
TL;DR: Stem cell therapy appears to be a promising area of research for cardiac regeneration following ischemic heart failure, but full maturity of cardiomyocytes remains elusive and will remain the main challenge for stem cell therapy in the near future.
Book ChapterDOI
Human Embryonic Stem Cells in Regenerative Medicine
Odessa Yabut,Harold S. Bernstein +1 more
TL;DR: This chapter discusses how hESC lines are derived, the means by which pluripotency is monitored, and how their ability to differentiate into all three embryonic germ layers is determined, and the methods currently employed to direct their differentiation into populations of tissue-specific, functional cells.
References
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Journal ArticleDOI
Embryonic Stem Cell Lines Derived from Human Blastocysts
James A. Thomson,Joseph Itskovitz-Eldor,Sander S. Shapiro,Michelle A. Waknitz,Swiergiel Jennifer J,Vivienne S. Marshall,Jeffrey M. Jones +6 more
TL;DR: Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages.
Journal ArticleDOI
The serial cultivation of human diploid cell strains.
Leonard Hayflick,P.S. Moorhead +1 more
TL;DR: A consideration of the cause of the eventual degeneration of these strains leads to the hypothesis that non-cumulative external factors are excluded and that the phenomenon is attributable to intrinsic factors which are expressed as senescence at the cellular level.
Journal ArticleDOI
Formation of Pluripotent Stem Cells in the Mammalian Embryo Depends on the POU Transcription Factor Oct4
Jennifer Nichols,Branko Zevnik,Konstantinos Anastassiadis,Hitoshi Niwa,Daniela Klewe-Nebenius,Ian Chambers,Hans R. Schöler,Austin Smith +7 more
TL;DR: It is reported that the activity of Oct4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo and also determines paracrine growth factor signaling from stem cells to the trophectoderm.
Journal ArticleDOI
Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro.
TL;DR: The derivation of pluripotent embryonic stem (ES) cells from human blastocysts is described, providing a model to study early human embryology, an investigational tool for discovery of novel growth factors and medicines, and a potential source of cells for use in transplantation therapy.
Journal ArticleDOI
Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers.
Joseph Itskovitz-Eldor,Maya Schuldiner,Dorit Karsenti,Amir Eden,Ofra Yanuka,Michal Amit,Hermona Soreq,Nissim Benvenisty +7 more
TL;DR: The ability to induce formation of human embryoid bodies that contain cells of neuronal, hematopoietic and cardiac origins will be useful in studying early human embryonic development as well as in transplantation medicine.