Journal ArticleDOI
Derivation of embryonic stem-cell lines from human blastocysts.
Chad A. Cowan,Irinha Klimanskaya,Jill A. McMahon,Jocelyn Atienza,Jeannine Witmyer,Jacob P. Zucker,Shunping Wang,Cynthia C. Morton,Andrew P. McMahon,Doug Powers,Douglas A. Melton +10 more
TLDR
The procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts are discussed.Abstract:
This report, first published online on March 3, 2004, discusses the procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts. These cell lines are available to researchers under a Material Transfer Agreement; according to current regulations, the cells cannot be used for research supported by federal funds. These cells are expected to facilitate research on a variety of serious chronic diseases.read more
Citations
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Journal ArticleDOI
Current techniques in reprogramming cell potency.
Firdaus Romli,Noorjahan Banu Alitheen,Muhajir Hamid,Ruzila Ismail,Nik-Mohd-Afizan Nik Abd. Rahman +4 more
TL;DR: This review addresses the various techniques made available to create iPSC with problems hogging down the technique.
Liver development and in vitro differentiation of embryonic stem cells to hepatocytes
TL;DR: The process of liver development including molecules and growth factors incorporate in the liver development as a template for in vitro differentiation of mouse and human embryonic stem cells is summarized and related studies and techniques for analyzing functionality of differentiated cells are discussed.
Journal ArticleDOI
Pluripotent stem cells: Maintenance of genetic and epigenetic stability and prospects of cell technologies
O. F. Gordeeva,Sh. M. Mitalipov +1 more
TL;DR: This review summarizes the research data on the genetic and epigenetic stability of different lines of pluripotent stem cells after long-term in vitro culture and considers the prospects of using pluripotency of different origin in cell therapy and pharmacological studies.
Journal Article
The effect of homocysteine thiolactone on acetylcholinesterase activity in rat brain, blood and heart
Milan Petrovic,Ivan Fufanovic,Iris Elezovic,Mirjana B. Čolović,Danijela Krstić,Vladimir Jakovljevic,Dragan Djuric +6 more
TL;DR: It was concluded that homocysteine thiolactone significantly inhibited AChE activity in the heart and brain tissue, but not in the blood of the rat.
Dissertation
FGF signaling in specification of hESC-derived definitive endoderm
TL;DR: Two studies investigate the role of fibroblast growth factor (FGF) signaling, specifically FGF2 and FGF4, and Retinoic acid (RA) in specification of the hESC-derived DE development and present a method for efficient gene targeting in hESCs, which allows the monitoring of gene expression in living cells.
References
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Journal ArticleDOI
Embryonic Stem Cell Lines Derived from Human Blastocysts
James A. Thomson,Joseph Itskovitz-Eldor,Sander S. Shapiro,Michelle A. Waknitz,Swiergiel Jennifer J,Vivienne S. Marshall,Jeffrey M. Jones +6 more
TL;DR: Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages.
Journal ArticleDOI
The serial cultivation of human diploid cell strains.
Leonard Hayflick,P.S. Moorhead +1 more
TL;DR: A consideration of the cause of the eventual degeneration of these strains leads to the hypothesis that non-cumulative external factors are excluded and that the phenomenon is attributable to intrinsic factors which are expressed as senescence at the cellular level.
Journal ArticleDOI
Formation of Pluripotent Stem Cells in the Mammalian Embryo Depends on the POU Transcription Factor Oct4
Jennifer Nichols,Branko Zevnik,Konstantinos Anastassiadis,Hitoshi Niwa,Daniela Klewe-Nebenius,Ian Chambers,Hans R. Schöler,Austin Smith +7 more
TL;DR: It is reported that the activity of Oct4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo and also determines paracrine growth factor signaling from stem cells to the trophectoderm.
Journal ArticleDOI
Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro.
TL;DR: The derivation of pluripotent embryonic stem (ES) cells from human blastocysts is described, providing a model to study early human embryology, an investigational tool for discovery of novel growth factors and medicines, and a potential source of cells for use in transplantation therapy.
Journal ArticleDOI
Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers.
Joseph Itskovitz-Eldor,Maya Schuldiner,Dorit Karsenti,Amir Eden,Ofra Yanuka,Michal Amit,Hermona Soreq,Nissim Benvenisty +7 more
TL;DR: The ability to induce formation of human embryoid bodies that contain cells of neuronal, hematopoietic and cardiac origins will be useful in studying early human embryonic development as well as in transplantation medicine.