Journal ArticleDOI
Derivation of embryonic stem-cell lines from human blastocysts.
Chad A. Cowan,Irinha Klimanskaya,Jill A. McMahon,Jocelyn Atienza,Jeannine Witmyer,Jacob P. Zucker,Shunping Wang,Cynthia C. Morton,Andrew P. McMahon,Doug Powers,Douglas A. Melton +10 more
TLDR
The procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts are discussed.Abstract:
This report, first published online on March 3, 2004, discusses the procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts. These cell lines are available to researchers under a Material Transfer Agreement; according to current regulations, the cells cannot be used for research supported by federal funds. These cells are expected to facilitate research on a variety of serious chronic diseases.read more
Citations
More filters
Journal Article
Chemically diverse polymer microarrays and high throughput surface characterisation: a method for discovery of materials for stem cell culture
Adam D. Celiz,James G W Smith,Asha K. Patel,Robert Langer,Daniel G. Anderson,David A. Barrett,Lorraine E. Young,Martyn C. Davies,Chris Denning,Morgan R. Alexander +9 more
TL;DR: In this article, the authors show that it is possible to print and assess cell adhesion of 141 different monomers in a microarray format, allowing access to the largest chemical space to date, allowing to meet the regenerative medicine challenge to provide scalable synthetic culture ware.
Book ChapterDOI
Culturing Human Pluripotent Stem Cells on a Feeder Layer
TL;DR: This chapter addresses the techniques necessary to culture hPSCs on a feeder layer and will help you gain a better understanding of the basic culture methods of hPSC cultured on mitotically inactivated feeder layers.
Journal ArticleDOI
Transfection of hPSC-Cardiomyocytes Using Viafect™ Transfection Reagent.
TL;DR: This protocol greatly adds value to the field by overcoming limited transfection efficiencies of hPSC-CMs in a simple and quick approach that ensures sustained expression of transfected genes for at least 14 days, opening new opportunities in mechanistic discovery for cardiac-related diseases.
Dissertation
Improving the mesodermal differentiation potential of human embryonic stem cells
TL;DR: This thesis aims to test the hypothesis that formation of hESC derivatives is regulated by the same mechanisms and ontology as in vivo embryo development, and can be optimised to maximise the production of mesoderm, and, ultimately, Mesoderm derivatives.
Journal ArticleDOI
Normal human embryonic stem cell lines were derived from microsurgical enucleated tripronuclear zygotes.
Chunyan Jiang,Lingbo Cai,Boxian Huang,Boxian Huang,Juan Dong,Aiqin Chen,Song Ning,Yugui Cui,Lianju Qin,Jiayin Liu,Jiayin Liu +10 more
TL;DR: By microsurgical pronuclear removal, this study indicates that depronucleared 3PN zygotes can improve the blastocysts formation rate, and normal hESC lines can be derived from those corrected 2PN embryos.
References
More filters
Journal ArticleDOI
Embryonic Stem Cell Lines Derived from Human Blastocysts
James A. Thomson,Joseph Itskovitz-Eldor,Sander S. Shapiro,Michelle A. Waknitz,Swiergiel Jennifer J,Vivienne S. Marshall,Jeffrey M. Jones +6 more
TL;DR: Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages.
Journal ArticleDOI
The serial cultivation of human diploid cell strains.
Leonard Hayflick,P.S. Moorhead +1 more
TL;DR: A consideration of the cause of the eventual degeneration of these strains leads to the hypothesis that non-cumulative external factors are excluded and that the phenomenon is attributable to intrinsic factors which are expressed as senescence at the cellular level.
Journal ArticleDOI
Formation of Pluripotent Stem Cells in the Mammalian Embryo Depends on the POU Transcription Factor Oct4
Jennifer Nichols,Branko Zevnik,Konstantinos Anastassiadis,Hitoshi Niwa,Daniela Klewe-Nebenius,Ian Chambers,Hans R. Schöler,Austin Smith +7 more
TL;DR: It is reported that the activity of Oct4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo and also determines paracrine growth factor signaling from stem cells to the trophectoderm.
Journal ArticleDOI
Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro.
TL;DR: The derivation of pluripotent embryonic stem (ES) cells from human blastocysts is described, providing a model to study early human embryology, an investigational tool for discovery of novel growth factors and medicines, and a potential source of cells for use in transplantation therapy.
Journal ArticleDOI
Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers.
Joseph Itskovitz-Eldor,Maya Schuldiner,Dorit Karsenti,Amir Eden,Ofra Yanuka,Michal Amit,Hermona Soreq,Nissim Benvenisty +7 more
TL;DR: The ability to induce formation of human embryoid bodies that contain cells of neuronal, hematopoietic and cardiac origins will be useful in studying early human embryonic development as well as in transplantation medicine.