Journal ArticleDOI
Derivation of embryonic stem-cell lines from human blastocysts.
Chad A. Cowan,Irinha Klimanskaya,Jill A. McMahon,Jocelyn Atienza,Jeannine Witmyer,Jacob P. Zucker,Shunping Wang,Cynthia C. Morton,Andrew P. McMahon,Doug Powers,Douglas A. Melton +10 more
TLDR
The procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts are discussed.Abstract:
This report, first published online on March 3, 2004, discusses the procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts. These cell lines are available to researchers under a Material Transfer Agreement; according to current regulations, the cells cannot be used for research supported by federal funds. These cells are expected to facilitate research on a variety of serious chronic diseases.read more
Citations
More filters
Journal ArticleDOI
Isolation of amniotic stem cell lines with potential for therapy.
Paolo De Coppi,Georg Bartsch,M. Minhaj Siddiqui,Tao Xu,Cesar C. Santos,Laura Perin,Gustavo Mostoslavsky,Ang line C. Serre,Evan Y. Snyder,James J. Yoo,Mark E. Furth,Shay Soker,Anthony Atala +12 more
TL;DR: The isolation of human and rodent amniotic fluid–derived stem (AFS) cells that express embryonic and adult stem cell markers are reported and examples of differentiated cells derived from human AFS cells and displaying specialized functions include neuronal lineage cells secreting the neurotransmitter L-glutamate or expressing G-protein-gated inwardly rectifying potassium channels.
Journal ArticleDOI
Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2.
Danwei Huangfu,Kenji Osafune,René Maehr,Wenjun Guo,Astrid Eijkelenboom,Astrid Eijkelenboom,Shuibing Chen,Whitney Muhlestein,Douglas A. Melton +8 more
TL;DR: Valproic acid (VPA), a histone deacetylase inhibitor, enables reprogramming of primary human fibroblasts with only two factors, Oct4 and Sox2, without the need for the oncogenes c-Myc or Klf4, and supports the possibility of reprograming through purely chemical means.
Journal ArticleDOI
Stochastic State Transitions Give Rise to Phenotypic Equilibrium in Populations of Cancer Cells
Piyush Gupta,Christine M. Fillmore,Guozhi Jiang,Sagi Shapira,Kai Tao,Charlotte Kuperwasser,Charlotte Kuperwasser,Eric S. Lander,Eric S. Lander,Eric S. Lander +9 more
TL;DR: It is shown that subpopulations of cells purified for a given phenotypic state return towards equilibrium proportions over time, and this findings contribute to the understanding of cancer heterogeneity and reveal how stochasticity in single-cell behaviors promotes phenotypesic equilibrium in populations of cancer cells.
Journal ArticleDOI
Somatic coding mutations in human induced pluripotent stem cells
Athurva Gore,Zhe Li,Ho Lim Fung,Jessica E. Young,Suneet Agarwal,Jessica Antosiewicz-Bourget,Isabel Canto,Alessandra Giorgetti,Mason A. Israel,Evangelos Kiskinis,Je-Hyuk Lee,Yuin-Han Loh,Philip D. Manos,Nuria Montserrat,Athanasia D. Panopoulos,Sergio Ruiz,Melissa L. Wilbert,Junying Yu,Ewen F. Kirkness,Juan Carlos Izpisua Belmonte,Derrick J. Rossi,James A. Thomson,Kevin Eggan,George Q. Daley,Lawrence S.B. Goldstein,Kun Zhang +25 more
TL;DR: It is shown that 22 human induced pluripotent stem (hiPS) cell lines reprogrammed using five different methods each contained an average of five protein-coding point mutations in the regions sampled, and that hiPS cells acquire genetic modifications in addition to epigenetic modifications.
Book
Immunogenicity of Induced Pluripotent Stem Cells
TL;DR: Findings indicate that, in contrast to derivatives of ESCs, abnormal gene expression in some cells differentiated from iPSCs can induce T-cell-dependent immune response in syngeneic recipients.
References
More filters
Journal ArticleDOI
Embryonic Stem Cell Lines Derived from Human Blastocysts
James A. Thomson,Joseph Itskovitz-Eldor,Sander S. Shapiro,Michelle A. Waknitz,Swiergiel Jennifer J,Vivienne S. Marshall,Jeffrey M. Jones +6 more
TL;DR: Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages.
Journal ArticleDOI
The serial cultivation of human diploid cell strains.
Leonard Hayflick,P.S. Moorhead +1 more
TL;DR: A consideration of the cause of the eventual degeneration of these strains leads to the hypothesis that non-cumulative external factors are excluded and that the phenomenon is attributable to intrinsic factors which are expressed as senescence at the cellular level.
Journal ArticleDOI
Formation of Pluripotent Stem Cells in the Mammalian Embryo Depends on the POU Transcription Factor Oct4
Jennifer Nichols,Branko Zevnik,Konstantinos Anastassiadis,Hitoshi Niwa,Daniela Klewe-Nebenius,Ian Chambers,Hans R. Schöler,Austin Smith +7 more
TL;DR: It is reported that the activity of Oct4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo and also determines paracrine growth factor signaling from stem cells to the trophectoderm.
Journal ArticleDOI
Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro.
TL;DR: The derivation of pluripotent embryonic stem (ES) cells from human blastocysts is described, providing a model to study early human embryology, an investigational tool for discovery of novel growth factors and medicines, and a potential source of cells for use in transplantation therapy.
Journal ArticleDOI
Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers.
Joseph Itskovitz-Eldor,Maya Schuldiner,Dorit Karsenti,Amir Eden,Ofra Yanuka,Michal Amit,Hermona Soreq,Nissim Benvenisty +7 more
TL;DR: The ability to induce formation of human embryoid bodies that contain cells of neuronal, hematopoietic and cardiac origins will be useful in studying early human embryonic development as well as in transplantation medicine.