Journal ArticleDOI
Derivation of embryonic stem-cell lines from human blastocysts.
Chad A. Cowan,Irinha Klimanskaya,Jill A. McMahon,Jocelyn Atienza,Jeannine Witmyer,Jacob P. Zucker,Shunping Wang,Cynthia C. Morton,Andrew P. McMahon,Doug Powers,Douglas A. Melton +10 more
TLDR
The procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts are discussed.Abstract:
This report, first published online on March 3, 2004, discusses the procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts. These cell lines are available to researchers under a Material Transfer Agreement; according to current regulations, the cells cannot be used for research supported by federal funds. These cells are expected to facilitate research on a variety of serious chronic diseases.read more
Citations
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Book ChapterDOI
1 – Current and Future Perspectives of Regenerative Medicine
Mark E. Furth,Anthony Atala +1 more
Journal ArticleDOI
Genomic integrity of human induced pluripotent stem cells: Reprogramming, differentiation and applications.
TL;DR: The impact of reprograming parameters such as starting cell type and reprogramming method on the maintenance of the cellular genomic integrity and the impact of genomic alterations on the possible usages of hiPSCs and their derivatives are discussed.
Journal ArticleDOI
Trace levels of mitomycin C disrupt genomic integrity and lead to DNA damage response defect in long-term-cultured human embryonic stem cells
Di Zhou,Ge Lin,Sicong Zeng,Bo Xiong,Pingyuan Xie,Dehua Cheng,Qing Zheng,Qi Ouyang,Xiaoying Zhou,Wei-Ling Tang,Yi Sun,Guang-Ying Lu,Lu Guangxiu +12 more
TL;DR: DDR genes were down-regulated during long-term culture in the MMC-containing system, leading to DDR defect and shortened telomeres of hES cells, a sign of genomic instability, which would limit hES from being applied for future clinic use and drug screening.
Book ChapterDOI
Epigenetic Regulation of Pluripotency
TL;DR: This chapter provides an overview of epigenetic regulation in embryonic stem cells and presents a brief introduction into epigenetic mechanisms and focus on their role in pluripotent cells.
Journal ArticleDOI
MicroRNA-934 is a novel primate-specific small non-coding RNA with neurogenic function during early development.
Kanella Prodromidou,Ioannis S. Vlachos,Maria Gaitanou,Georgia Kouroupi,Artemis G. Hatzigeorgiou,Rebecca Matsas +5 more
TL;DR: It is found that miR-934 directly controls progenitor to neuroblast transition and impacts on neurite growth of newborn neurons, suggesting that mir- 934 is a novel regulator of early human neurogenesis with potential implications for a species-specific evolutionary role in brain function.
References
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Journal ArticleDOI
Embryonic Stem Cell Lines Derived from Human Blastocysts
James A. Thomson,Joseph Itskovitz-Eldor,Sander S. Shapiro,Michelle A. Waknitz,Swiergiel Jennifer J,Vivienne S. Marshall,Jeffrey M. Jones +6 more
TL;DR: Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages.
Journal ArticleDOI
The serial cultivation of human diploid cell strains.
Leonard Hayflick,P.S. Moorhead +1 more
TL;DR: A consideration of the cause of the eventual degeneration of these strains leads to the hypothesis that non-cumulative external factors are excluded and that the phenomenon is attributable to intrinsic factors which are expressed as senescence at the cellular level.
Journal ArticleDOI
Formation of Pluripotent Stem Cells in the Mammalian Embryo Depends on the POU Transcription Factor Oct4
Jennifer Nichols,Branko Zevnik,Konstantinos Anastassiadis,Hitoshi Niwa,Daniela Klewe-Nebenius,Ian Chambers,Hans R. Schöler,Austin Smith +7 more
TL;DR: It is reported that the activity of Oct4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo and also determines paracrine growth factor signaling from stem cells to the trophectoderm.
Journal ArticleDOI
Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro.
TL;DR: The derivation of pluripotent embryonic stem (ES) cells from human blastocysts is described, providing a model to study early human embryology, an investigational tool for discovery of novel growth factors and medicines, and a potential source of cells for use in transplantation therapy.
Journal ArticleDOI
Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers.
Joseph Itskovitz-Eldor,Maya Schuldiner,Dorit Karsenti,Amir Eden,Ofra Yanuka,Michal Amit,Hermona Soreq,Nissim Benvenisty +7 more
TL;DR: The ability to induce formation of human embryoid bodies that contain cells of neuronal, hematopoietic and cardiac origins will be useful in studying early human embryonic development as well as in transplantation medicine.