Journal ArticleDOI
Derivation of embryonic stem-cell lines from human blastocysts.
Chad A. Cowan,Irinha Klimanskaya,Jill A. McMahon,Jocelyn Atienza,Jeannine Witmyer,Jacob P. Zucker,Shunping Wang,Cynthia C. Morton,Andrew P. McMahon,Doug Powers,Douglas A. Melton +10 more
TLDR
The procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts are discussed.Abstract:
This report, first published online on March 3, 2004, discusses the procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts. These cell lines are available to researchers under a Material Transfer Agreement; according to current regulations, the cells cannot be used for research supported by federal funds. These cells are expected to facilitate research on a variety of serious chronic diseases.read more
Citations
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Dissertation
Developing a model system to investigate the epigenetic mechanisms underlying pluripotency in human cells
TL;DR: New insights are gained into the epigenetic mechanisms underlying cell phenotype and provided the foundation for further improving reprogramming efficiency, including the first report for SOX2 differential methylation in human non-cancerous cells.
Investigating the role of retinoblastoma-binding protein 9 in human pluripotent stem cells and embryonic development
TL;DR: The present work focuses on hiPSCs: advancements in cell reprogramming and disease modelling, and core regulatory factors controlling pluripotency in hESCs, a model of embryonic development based on zebrafish embryogenesis.
Journal ArticleDOI
Human embryonic stem cell–derived fibroblastic and epitheloid lineages as xeno-free support?
TL;DR: A novel approach would be to examine the possible use of hES cell–derived fibroblastic and epitheloid lineages as feeder supports as well as the use of donated human tissues to develop a xeno-free support system for hES cells.
Journal ArticleDOI
Establishment of a feeder and serum-free culture system for human embryonic stem cells.
Liyun Wang,RuiNa Zhang,Ronghua Ma,Gong-Xue Jia,Shengyan Jian,Xianghui Zeng,Zhengfang Xiong,Binye Li,Chen Li,ZhenZhen Lv,Xue Bai +10 more
TL;DR: A feeder-free, serum-free culture method for human embryonic stem cells (hESCs), to establish optimal conditions for hESC proliferation, and to determine the biological characteristics of the resulting hESCs maintained the self-renewal potential and pluripotency of H9 cells for eight passages.
Journal ArticleDOI
Identification of an epigenetic signature in human induced pluripotent stem cells using a linear machine learning model
Koichiro Nishino,Ken Takasawa,Kohji Okamura,Yoshikazu Arai,Asato Sekiya,Hidenori Akutsu,Akihiro Umezawa +6 more
TL;DR: A linear classification-based learning model is established to distinguish among ESCs, iPSCs, embryonal carcinoma cells (ECCs), and somatic cells on the basis of their DNA methylation profiles.
References
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Journal ArticleDOI
Embryonic Stem Cell Lines Derived from Human Blastocysts
James A. Thomson,Joseph Itskovitz-Eldor,Sander S. Shapiro,Michelle A. Waknitz,Swiergiel Jennifer J,Vivienne S. Marshall,Jeffrey M. Jones +6 more
TL;DR: Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages.
Journal ArticleDOI
The serial cultivation of human diploid cell strains.
Leonard Hayflick,P.S. Moorhead +1 more
TL;DR: A consideration of the cause of the eventual degeneration of these strains leads to the hypothesis that non-cumulative external factors are excluded and that the phenomenon is attributable to intrinsic factors which are expressed as senescence at the cellular level.
Journal ArticleDOI
Formation of Pluripotent Stem Cells in the Mammalian Embryo Depends on the POU Transcription Factor Oct4
Jennifer Nichols,Branko Zevnik,Konstantinos Anastassiadis,Hitoshi Niwa,Daniela Klewe-Nebenius,Ian Chambers,Hans R. Schöler,Austin Smith +7 more
TL;DR: It is reported that the activity of Oct4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo and also determines paracrine growth factor signaling from stem cells to the trophectoderm.
Journal ArticleDOI
Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro.
TL;DR: The derivation of pluripotent embryonic stem (ES) cells from human blastocysts is described, providing a model to study early human embryology, an investigational tool for discovery of novel growth factors and medicines, and a potential source of cells for use in transplantation therapy.
Journal ArticleDOI
Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers.
Joseph Itskovitz-Eldor,Maya Schuldiner,Dorit Karsenti,Amir Eden,Ofra Yanuka,Michal Amit,Hermona Soreq,Nissim Benvenisty +7 more
TL;DR: The ability to induce formation of human embryoid bodies that contain cells of neuronal, hematopoietic and cardiac origins will be useful in studying early human embryonic development as well as in transplantation medicine.