Journal ArticleDOI
Derivation of embryonic stem-cell lines from human blastocysts.
Chad A. Cowan,Irinha Klimanskaya,Jill A. McMahon,Jocelyn Atienza,Jeannine Witmyer,Jacob P. Zucker,Shunping Wang,Cynthia C. Morton,Andrew P. McMahon,Doug Powers,Douglas A. Melton +10 more
TLDR
The procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts are discussed.Abstract:
This report, first published online on March 3, 2004, discusses the procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts. These cell lines are available to researchers under a Material Transfer Agreement; according to current regulations, the cells cannot be used for research supported by federal funds. These cells are expected to facilitate research on a variety of serious chronic diseases.read more
Citations
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The ROCK inhibitor Y-27632 improves recovery of human embryonic stem cells after fluorescence-activated cell sorting with multiple cell surface markers.
Nil Emre,Jason G. Vidal,Jeanne Elia,Eric D. O'Connor,Rosanto I. Paramban,Michael P. Hefferan,Roman Navarro,Danielle Goldberg,Nissi Varki,Martin Marsala,Christian T. Carson +10 more
TL;DR: The application of Y-27632 to hESCs after cell sorting improves cell recovery with no observed effect on pluripotency, and enables the consistent recovery of hESC by FACS using multiple surface markers.
Journal ArticleDOI
Genome-wide reduction in H3K9 acetylation during human embryonic stem cell differentiation.
Jana Krejčí,Radka Uhlírová,Gabriela Galiová,Stanislav Kozubek,Jana Šmigová,Jana Šmigová,Eva Bártová +6 more
TL;DR: ChIP‐on‐chip analysis revealed that differentiation results in a genome‐wide decrease in promoter H3K9 acetylation, and analyses point to chromosomes 11, 12, 17, and 19 as being critical for hESC pluripotency and endoderm‐like differentiation.
Journal ArticleDOI
Peripheral sensory neurons differentiate from neural precursors derived from human embryonic stem cells
Irina Brokhman,Lina Gamarnik-Ziegler,Oz Pomp,Michal Aharonowiz,Benjamin Reubinoff,Ronald S. Goldstein +5 more
TL;DR: These results provide the first evidence that neural precursors derived from hESC have the potential to develop into PS neurons-like as well as CNS-like neuronal cells.
Journal ArticleDOI
Activin B mediated induction of Pdx1 in human embryonic stem cell derived embryoid bodies
Ulrik Frandsen,Ann Dorte Storm Pørneki,Charlotte Floridon,Basem M. Abdallah,Moustapha Kassem +4 more
TL;DR: It is reported here that Activin B in a dose depend manner markedly up-regulates Pdx1 expression as compared to Activin A and untreated cultures, suggesting that additional suppression of Shh signaling may be required to allow for proper specification of pancreatic cell lineages in hESCs.
Journal ArticleDOI
The Importance of Ubiquitination and Deubiquitination in Cellular Reprogramming
TL;DR: This review provides a relatively new understanding regarding the importance of ubiquitination/deubiquitination of stem cell transcription factors for efficient cellular reprogramming and the possibility of using DUBs, along with core transcription factors, to efficiently generate induced pluripotent stem cells.
References
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Journal ArticleDOI
Embryonic Stem Cell Lines Derived from Human Blastocysts
James A. Thomson,Joseph Itskovitz-Eldor,Sander S. Shapiro,Michelle A. Waknitz,Swiergiel Jennifer J,Vivienne S. Marshall,Jeffrey M. Jones +6 more
TL;DR: Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages.
Journal ArticleDOI
The serial cultivation of human diploid cell strains.
Leonard Hayflick,P.S. Moorhead +1 more
TL;DR: A consideration of the cause of the eventual degeneration of these strains leads to the hypothesis that non-cumulative external factors are excluded and that the phenomenon is attributable to intrinsic factors which are expressed as senescence at the cellular level.
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Formation of Pluripotent Stem Cells in the Mammalian Embryo Depends on the POU Transcription Factor Oct4
Jennifer Nichols,Branko Zevnik,Konstantinos Anastassiadis,Hitoshi Niwa,Daniela Klewe-Nebenius,Ian Chambers,Hans R. Schöler,Austin Smith +7 more
TL;DR: It is reported that the activity of Oct4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo and also determines paracrine growth factor signaling from stem cells to the trophectoderm.
Journal ArticleDOI
Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro.
TL;DR: The derivation of pluripotent embryonic stem (ES) cells from human blastocysts is described, providing a model to study early human embryology, an investigational tool for discovery of novel growth factors and medicines, and a potential source of cells for use in transplantation therapy.
Journal ArticleDOI
Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers.
Joseph Itskovitz-Eldor,Maya Schuldiner,Dorit Karsenti,Amir Eden,Ofra Yanuka,Michal Amit,Hermona Soreq,Nissim Benvenisty +7 more
TL;DR: The ability to induce formation of human embryoid bodies that contain cells of neuronal, hematopoietic and cardiac origins will be useful in studying early human embryonic development as well as in transplantation medicine.