Journal ArticleDOI
Derivation of embryonic stem-cell lines from human blastocysts.
Chad A. Cowan,Irinha Klimanskaya,Jill A. McMahon,Jocelyn Atienza,Jeannine Witmyer,Jacob P. Zucker,Shunping Wang,Cynthia C. Morton,Andrew P. McMahon,Doug Powers,Douglas A. Melton +10 more
TLDR
The procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts are discussed.Abstract:
This report, first published online on March 3, 2004, discusses the procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts. These cell lines are available to researchers under a Material Transfer Agreement; according to current regulations, the cells cannot be used for research supported by federal funds. These cells are expected to facilitate research on a variety of serious chronic diseases.read more
Citations
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The nature and nurture of cell heterogeneity: accounting for macrophage gene-environment interactions with single-cell RNA-Seq.
Quin F. Wills,Quin F. Wills,Esther Mellado-Gomez,Rory Nolan,Rory Nolan,Damien Warner,Eshita Sharma,John Broxholme,Benjamin Wright,Helen Lockstone,William James,Mark Lynch,Michael L. Gonzales,Jay A. A. West,Anne A. Leyrat,Sergi Padilla-Parra,Sergi Padilla-Parra,Sarah Filippi,Christopher Holmes,Christopher Holmes,Michael D. Moore,Rory Bowden +21 more
TL;DR: Using gene-edited macrophages, it is demonstrated how previously unappreciated knockout effects of SAMHD1, such as an altered oxidative stress response, have a large paracrine signaling component.
Journal ArticleDOI
Induced pluripotent stem cell research: A revolutionary approach to face the challenges in drug screening
TL;DR: The history and progress of induced pluripotent stem (iPS) cells is summarized, support for the growing interest in iPS cell applications is provided, with emphasis on practical uses in cell-based drug screening, and some challenges faced in the use of this technology are discussed.
Journal ArticleDOI
Genetically variant human pluripotent stem cells selectively eliminate wild-type counterparts through YAP-mediated cell competition.
Christopher J. Price,Dylan Stavish,Paul J. Gokhale,Ben A. Stevenson,Samantha Sargeant,Joanne Lacey,Tristan A. Rodriguez,Ivana Barbaric +7 more
TL;DR: In this paper, the dominance of variant clones in mosaic cultures is enhanced through competitive interactions that result in the elimination of wild-type cells through corralling and mechanical compression by faster-growing variants, causing a redistribution of F-actin and sequestration of yes-associated protein (YAP) in the cytoplasm that induces apoptosis in wild type cells.
Journal ArticleDOI
Kidney organogenesis and regeneration: a new era in the treatment of chronic renal failure?
TL;DR: This work reviews recent progress in the field of regenerative nephrology, focusing on the de novo establishment of a whole kidney.
Journal ArticleDOI
Safety of human embryonic stem cells in patients with terminal/incurable conditions- a retrospective analysis.
Geeta Shroff,J. K Barthakur +1 more
TL;DR: A single cohort study conducted at Nutech Mediworld, New Delhi as discussed by the authors was aimed at establishing the safety of human embryonic stem cells (hESC) therapy in patients with terminal/incurable conditions.
References
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Journal ArticleDOI
Embryonic Stem Cell Lines Derived from Human Blastocysts
James A. Thomson,Joseph Itskovitz-Eldor,Sander S. Shapiro,Michelle A. Waknitz,Swiergiel Jennifer J,Vivienne S. Marshall,Jeffrey M. Jones +6 more
TL;DR: Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages.
Journal ArticleDOI
The serial cultivation of human diploid cell strains.
Leonard Hayflick,P.S. Moorhead +1 more
TL;DR: A consideration of the cause of the eventual degeneration of these strains leads to the hypothesis that non-cumulative external factors are excluded and that the phenomenon is attributable to intrinsic factors which are expressed as senescence at the cellular level.
Journal ArticleDOI
Formation of Pluripotent Stem Cells in the Mammalian Embryo Depends on the POU Transcription Factor Oct4
Jennifer Nichols,Branko Zevnik,Konstantinos Anastassiadis,Hitoshi Niwa,Daniela Klewe-Nebenius,Ian Chambers,Hans R. Schöler,Austin Smith +7 more
TL;DR: It is reported that the activity of Oct4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo and also determines paracrine growth factor signaling from stem cells to the trophectoderm.
Journal ArticleDOI
Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro.
TL;DR: The derivation of pluripotent embryonic stem (ES) cells from human blastocysts is described, providing a model to study early human embryology, an investigational tool for discovery of novel growth factors and medicines, and a potential source of cells for use in transplantation therapy.
Journal ArticleDOI
Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers.
Joseph Itskovitz-Eldor,Maya Schuldiner,Dorit Karsenti,Amir Eden,Ofra Yanuka,Michal Amit,Hermona Soreq,Nissim Benvenisty +7 more
TL;DR: The ability to induce formation of human embryoid bodies that contain cells of neuronal, hematopoietic and cardiac origins will be useful in studying early human embryonic development as well as in transplantation medicine.