Journal ArticleDOI
Derivation of embryonic stem-cell lines from human blastocysts.
Chad A. Cowan,Irinha Klimanskaya,Jill A. McMahon,Jocelyn Atienza,Jeannine Witmyer,Jacob P. Zucker,Shunping Wang,Cynthia C. Morton,Andrew P. McMahon,Doug Powers,Douglas A. Melton +10 more
TLDR
The procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts are discussed.Abstract:
This report, first published online on March 3, 2004, discusses the procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts. These cell lines are available to researchers under a Material Transfer Agreement; according to current regulations, the cells cannot be used for research supported by federal funds. These cells are expected to facilitate research on a variety of serious chronic diseases.read more
Citations
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Human embryonic stem cell derivation from poor-quality embryos
Paul H. Lerou,Akiko Yabuuchi,Akiko Yabuuchi,Hongguang Huo,Hongguang Huo,Ayumu Takeuchi,Ayumu Takeuchi,Jessica Shea,Tina Cimini,Tan A. Ince,Elizabeth S. Ginsburg,Catherine Racowsky,George Q. Daley +12 more
TL;DR: Early-arrested or highly fragmented embryos only rarely yield cell lines, whereas those that have achieved blastocyst stage are a robust source of normal hES cells.
Journal ArticleDOI
Efficient multicistronic expression of a transgene in human embryonic stem cells.
TL;DR: Efficient multicistronic expression of the transgene was permitted by 2A‐mediated separation with almost the same amounts of encoded proteins in hESC and this technology may be a significant advance in the genetic engineering of hESCs and h ESC‐derived cells for purposes that require the reliable expression of multiple transgenes.
Journal ArticleDOI
Pluripotent Stem Cells: Current Understanding and Future Directions
Antonio Romito,Gilda Cobellis +1 more
TL;DR: A detailed overview of the family of pluripotent cell lines derived from early mouse and human embryos and compare them with induced pluripotency is provided, offering a comprehensive scenario of plurIPotent stem cells.
Journal ArticleDOI
Derivation of insulin-producing cells from human embryonic stem cells.
TL;DR: This review discusses the different protocols developed that are aimed at deriving beta-cells from hESCs and concludes that Mimicking normal pancreagenesis offers the best strategy for producing glucose-responsive insulin-producing cells in vitro for people with diabetes.
Journal ArticleDOI
Disruption and therapeutic rescue of autophagy in a human neuronal model of Niemann Pick type C1.
M. Paulina Ordonez,Elizabeth A. Roberts,Chelsea U. Kidwell,Shauna H. Yuan,Warren C. Plaisted,Lawrence S.B. Goldstein +5 more
TL;DR: It is suggested that neurons are especially sensitive to lysosomal cholesterol accumulation because of autophagy disruption and accumulation of fragmented mitochondria, thus defining a new route to effective drug development for NPC1 disease.
References
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Journal ArticleDOI
Embryonic Stem Cell Lines Derived from Human Blastocysts
James A. Thomson,Joseph Itskovitz-Eldor,Sander S. Shapiro,Michelle A. Waknitz,Swiergiel Jennifer J,Vivienne S. Marshall,Jeffrey M. Jones +6 more
TL;DR: Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages.
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The serial cultivation of human diploid cell strains.
Leonard Hayflick,P.S. Moorhead +1 more
TL;DR: A consideration of the cause of the eventual degeneration of these strains leads to the hypothesis that non-cumulative external factors are excluded and that the phenomenon is attributable to intrinsic factors which are expressed as senescence at the cellular level.
Journal ArticleDOI
Formation of Pluripotent Stem Cells in the Mammalian Embryo Depends on the POU Transcription Factor Oct4
Jennifer Nichols,Branko Zevnik,Konstantinos Anastassiadis,Hitoshi Niwa,Daniela Klewe-Nebenius,Ian Chambers,Hans R. Schöler,Austin Smith +7 more
TL;DR: It is reported that the activity of Oct4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo and also determines paracrine growth factor signaling from stem cells to the trophectoderm.
Journal ArticleDOI
Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro.
TL;DR: The derivation of pluripotent embryonic stem (ES) cells from human blastocysts is described, providing a model to study early human embryology, an investigational tool for discovery of novel growth factors and medicines, and a potential source of cells for use in transplantation therapy.
Journal ArticleDOI
Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers.
Joseph Itskovitz-Eldor,Maya Schuldiner,Dorit Karsenti,Amir Eden,Ofra Yanuka,Michal Amit,Hermona Soreq,Nissim Benvenisty +7 more
TL;DR: The ability to induce formation of human embryoid bodies that contain cells of neuronal, hematopoietic and cardiac origins will be useful in studying early human embryonic development as well as in transplantation medicine.