Journal ArticleDOI
Derivation of embryonic stem-cell lines from human blastocysts.
Chad A. Cowan,Irinha Klimanskaya,Jill A. McMahon,Jocelyn Atienza,Jeannine Witmyer,Jacob P. Zucker,Shunping Wang,Cynthia C. Morton,Andrew P. McMahon,Doug Powers,Douglas A. Melton +10 more
TLDR
The procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts are discussed.Abstract:
This report, first published online on March 3, 2004, discusses the procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts. These cell lines are available to researchers under a Material Transfer Agreement; according to current regulations, the cells cannot be used for research supported by federal funds. These cells are expected to facilitate research on a variety of serious chronic diseases.read more
Citations
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Quantitative analysis of DNA methylation of imprinted genes in single human blastocysts by pyrosequencing.
John Huntriss,Kathryn Woodfine,Joanna E. Huddleston,Adele Murrell,Anthony J. Rutherford,Kay Elder,Amir Ali Khan,Karen E Hemmings,Helen M. Picton +8 more
TL;DR: It is reported that imprints exist at KvDMR1, RB1, SNRPN, GRB10, and other imprinted loci in the human blastocyst and that RB1 and MEG3 were hypermethylated.
Journal ArticleDOI
Sources of cardiomyocytes for stem cell therapy: an update.
TL;DR: The view is that, even though the road ahead is long and tortuous, embryonic stem cells represent the most reliable font of CMC and the most promising type of cell to rely on for a future tool in heart failure treatment.
Book
Pancreatic Stem Cells
TL;DR: The pancreas is a unique organ that encompasses both endocrine and exocrine functions, including its innervation and vasculature, the exocrine acinar tissue, the ductal system, and the endocrine component, also termed the islets of Langerhans.
Journal ArticleDOI
Chromosomal characterization of cryopreserved mesenchymal stem cells from the human subendothelium umbilical cord vein
Denise M Duarte,Déborah Afonso Cornélio,Carolina Corado,Viviane K. S. Medeiros,Viviane K. S. Medeiros,Luíza A da Costa Xavier de Araújo,Geraldo B Cavalvanti,Silvia Regina Batistuzzo de Medeiros +7 more
TL;DR: The findings show that using mesenchymal stem cells for clinical approaches requires careful investigation and sensitive tests in order to ensure cellular therapy biosafety, and a significant number of nonclonal chromosomal aberrations were apparent after cryopreservation.
Journal ArticleDOI
Heterogeneity and randomness of DNA methylation patterns in human embryonic stem cells.
Albert G. Tsai,Debbie M Chen,Mayin Lin,John C. F. Hsieh,Cindy Y. Okitsu,Alexander Taghva,Darryl Shibata,Chih-Lin Hsieh +7 more
TL;DR: Traditional genomic bisulfite sequencing is carried out using the normal diploid human embryonic stem (hES) cell lines, and Hamming distance analysis is utilized to evaluate the existence of a distinct and nonrandom DNA methylation pattern at each locus studied, suggesting thatHamming distance is a simple, quick, and useful tool to identify loci with nonrandomDNA methylation patterns.
References
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Journal ArticleDOI
Embryonic Stem Cell Lines Derived from Human Blastocysts
James A. Thomson,Joseph Itskovitz-Eldor,Sander S. Shapiro,Michelle A. Waknitz,Swiergiel Jennifer J,Vivienne S. Marshall,Jeffrey M. Jones +6 more
TL;DR: Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages.
Journal ArticleDOI
The serial cultivation of human diploid cell strains.
Leonard Hayflick,P.S. Moorhead +1 more
TL;DR: A consideration of the cause of the eventual degeneration of these strains leads to the hypothesis that non-cumulative external factors are excluded and that the phenomenon is attributable to intrinsic factors which are expressed as senescence at the cellular level.
Journal ArticleDOI
Formation of Pluripotent Stem Cells in the Mammalian Embryo Depends on the POU Transcription Factor Oct4
Jennifer Nichols,Branko Zevnik,Konstantinos Anastassiadis,Hitoshi Niwa,Daniela Klewe-Nebenius,Ian Chambers,Hans R. Schöler,Austin Smith +7 more
TL;DR: It is reported that the activity of Oct4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo and also determines paracrine growth factor signaling from stem cells to the trophectoderm.
Journal ArticleDOI
Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro.
TL;DR: The derivation of pluripotent embryonic stem (ES) cells from human blastocysts is described, providing a model to study early human embryology, an investigational tool for discovery of novel growth factors and medicines, and a potential source of cells for use in transplantation therapy.
Journal ArticleDOI
Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers.
Joseph Itskovitz-Eldor,Maya Schuldiner,Dorit Karsenti,Amir Eden,Ofra Yanuka,Michal Amit,Hermona Soreq,Nissim Benvenisty +7 more
TL;DR: The ability to induce formation of human embryoid bodies that contain cells of neuronal, hematopoietic and cardiac origins will be useful in studying early human embryonic development as well as in transplantation medicine.