Journal ArticleDOI
Derivation of embryonic stem-cell lines from human blastocysts.
Chad A. Cowan,Irinha Klimanskaya,Jill A. McMahon,Jocelyn Atienza,Jeannine Witmyer,Jacob P. Zucker,Shunping Wang,Cynthia C. Morton,Andrew P. McMahon,Doug Powers,Douglas A. Melton +10 more
TLDR
The procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts are discussed.Abstract:
This report, first published online on March 3, 2004, discusses the procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts. These cell lines are available to researchers under a Material Transfer Agreement; according to current regulations, the cells cannot be used for research supported by federal funds. These cells are expected to facilitate research on a variety of serious chronic diseases.read more
Citations
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Embryonic stem cells from blastomeres maintaining embryo viability.
TL;DR: The blastomere biopsy-based technique allows the derivation of human ESClines without sacrificing a human embryo and was shown to be robust and produce safe and functional derivatives of therapeutic value.
Journal ArticleDOI
Purification of human muscle‐derived cells using an immunoselective method for potential use in urological regeneration
TL;DR: To purify human muscle‐derived cells (hMDCs) that could be used for managing urinary incontinence, erectile dysfunction and for bladder reconstitution.
Journal ArticleDOI
Vitrified blastocysts from Preimplantation Genetic Diagnosis (PGD) as a source for human Embryonic Stem Cell (hESC) derivation
B. Aran,Miquel Solé,Ignasi Rodríguez-Pizà,Mònica Parriego,Yolanda Muñoz,Montserrat Boada,Pere N. Barri,Juan Carlos Izpisua,Anna Veiga +8 more
TL;DR: Results showed better survival rates when blastocysts have not started the hatching process, and vitrification has proved to be an efficient method to preserve human Blastocysts.
Journal ArticleDOI
In Vivo Differentiated Human Embryonic Stem Cells Can Acquire Chromosomal Aberrations More Frequently Than In Vitro During the Same Period
M Zucchelli,Susanne Ström,Frida Holm,Helena Malmgren,Sigrid Sahlén,Piotr Religa,Outi Hovatta,Juha Kere,José Inzunza +8 more
TL;DR: Results indicate that mutations occur during the in vivo differentiation process as well as in vitro, and underlines the necessity to remove all pluripotent stem cells from the differentiated cell population that will be transplanted.
Journal ArticleDOI
Cannabinoids modulate cell survival in embryoid bodies.
Jader Nones,Tania Cristina Leite de Sampaio e Spohr,Daniel Rodrigues Furtado,Rafaela C. Sartore,Bruna S. Paulsen,Marília Zaluar P. Guimarães,Stevens K. Rehen +6 more
TL;DR: This study indicates that cannabinoid signalling is functionally implicated in the biology of differentiating ESCs, being the first to show that activation of cannabinoid receptors is able to increase cell viability via reduction of cell death rate in EBs.
References
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Journal ArticleDOI
Embryonic Stem Cell Lines Derived from Human Blastocysts
James A. Thomson,Joseph Itskovitz-Eldor,Sander S. Shapiro,Michelle A. Waknitz,Swiergiel Jennifer J,Vivienne S. Marshall,Jeffrey M. Jones +6 more
TL;DR: Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages.
Journal ArticleDOI
The serial cultivation of human diploid cell strains.
Leonard Hayflick,P.S. Moorhead +1 more
TL;DR: A consideration of the cause of the eventual degeneration of these strains leads to the hypothesis that non-cumulative external factors are excluded and that the phenomenon is attributable to intrinsic factors which are expressed as senescence at the cellular level.
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Formation of Pluripotent Stem Cells in the Mammalian Embryo Depends on the POU Transcription Factor Oct4
Jennifer Nichols,Branko Zevnik,Konstantinos Anastassiadis,Hitoshi Niwa,Daniela Klewe-Nebenius,Ian Chambers,Hans R. Schöler,Austin Smith +7 more
TL;DR: It is reported that the activity of Oct4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo and also determines paracrine growth factor signaling from stem cells to the trophectoderm.
Journal ArticleDOI
Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro.
TL;DR: The derivation of pluripotent embryonic stem (ES) cells from human blastocysts is described, providing a model to study early human embryology, an investigational tool for discovery of novel growth factors and medicines, and a potential source of cells for use in transplantation therapy.
Journal ArticleDOI
Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers.
Joseph Itskovitz-Eldor,Maya Schuldiner,Dorit Karsenti,Amir Eden,Ofra Yanuka,Michal Amit,Hermona Soreq,Nissim Benvenisty +7 more
TL;DR: The ability to induce formation of human embryoid bodies that contain cells of neuronal, hematopoietic and cardiac origins will be useful in studying early human embryonic development as well as in transplantation medicine.