Journal ArticleDOI
Derivation of embryonic stem-cell lines from human blastocysts.
Chad A. Cowan,Irinha Klimanskaya,Jill A. McMahon,Jocelyn Atienza,Jeannine Witmyer,Jacob P. Zucker,Shunping Wang,Cynthia C. Morton,Andrew P. McMahon,Doug Powers,Douglas A. Melton +10 more
TLDR
The procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts are discussed.Abstract:
This report, first published online on March 3, 2004, discusses the procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts. These cell lines are available to researchers under a Material Transfer Agreement; according to current regulations, the cells cannot be used for research supported by federal funds. These cells are expected to facilitate research on a variety of serious chronic diseases.read more
Citations
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Journal ArticleDOI
BCL-XL Mediates the Strong Selective Advantage of a 20q11.21 Amplification Commonly Found in Human Embryonic Stem Cell Cultures
Stuart Avery,Adam J. Hirst,Adam J. Hirst,Duncan Baker,Chin Yan Lim,Sharmini Alagaratnam,Rolf Inge Skotheim,Ragnhild A. Lothe,Martin F. Pera,Martin F. Pera,Alan Colman,Paul Robson,Peter W. Andrews,Barbara B. Knowles +13 more
TL;DR: It is shown that those containing this amplicon have higher population doubling rates, attributable to enhanced cell survival through resistance to apoptosis, and linking this mutation with malignant transformation.
Journal ArticleDOI
Embryonic stem cell-like cells derived from adult human testis
S.C. Mizrak,J.V. Chikhovskaya,Hooman Sadri-Ardekani,S.K.M. van Daalen,Cindy M. Korver,Suzanne Hovingh,Hermien L. Roepers-Gajadien,Angel Raya,K. Fluiter,Th. M. de Reijke,J.J.M.C.H. de la Rosette,Alida C. Knegt,Juan Carlos Izpisua Belmonte,F. van der Veen,Dirk G. de Rooij,Dirk G. de Rooij,Sjoerd Repping,A. M. M. Van Pelt +17 more
TL;DR: Multipotent cells can be established from adult human testis because of their easy accessibility and ethical acceptability as well as their non-tumorigenic and autogenic nature make these cells an attractive alternative to human ES cells for future stem cell therapies.
Journal ArticleDOI
Loss of viability during freeze-thaw of intact and adherent human embryonic stem cells with conventional slow-cooling protocols is predominantly due to apoptosis rather than cellular necrosis.
Boon Chin Heng,Chao Peng Ye,Hua Liu,Wei Seong Toh,Abdul Jalil Rufaihah,Zheng Yang,Boon-Huat Bay,Zigang Ge,Hongwei Ouyang,Eng Hin Lee,Tong Cao +10 more
TL;DR: It is demonstrated that apoptosis instead of cellular necrosis, is the major mechanism of the loss of viability of cryopreserved hES cells during freeze-thawing with conventional slow-cooling protocols.
Journal ArticleDOI
Culture conditions for human embryonic stem cells.
Heli Skottman,Outi Hovatta +1 more
TL;DR: Optimization and standardization of culture methods is needed for research as well as for clinical purposes to establish robust and cost-effective systems for culturing hESC cells and eliminate the risk of infection transmitted by animal pathogens and immunoreactions caused by animal substances in cell cultures before clinical treatment.
Journal ArticleDOI
Stem cells: science, policy, and ethics
TL;DR: The most viable stem cell lines and reliable ways to promote the differentiation of pluripotent stem cells into specific cell types are identified.
References
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Journal ArticleDOI
Embryonic Stem Cell Lines Derived from Human Blastocysts
James A. Thomson,Joseph Itskovitz-Eldor,Sander S. Shapiro,Michelle A. Waknitz,Swiergiel Jennifer J,Vivienne S. Marshall,Jeffrey M. Jones +6 more
TL;DR: Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages.
Journal ArticleDOI
The serial cultivation of human diploid cell strains.
Leonard Hayflick,P.S. Moorhead +1 more
TL;DR: A consideration of the cause of the eventual degeneration of these strains leads to the hypothesis that non-cumulative external factors are excluded and that the phenomenon is attributable to intrinsic factors which are expressed as senescence at the cellular level.
Journal ArticleDOI
Formation of Pluripotent Stem Cells in the Mammalian Embryo Depends on the POU Transcription Factor Oct4
Jennifer Nichols,Branko Zevnik,Konstantinos Anastassiadis,Hitoshi Niwa,Daniela Klewe-Nebenius,Ian Chambers,Hans R. Schöler,Austin Smith +7 more
TL;DR: It is reported that the activity of Oct4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo and also determines paracrine growth factor signaling from stem cells to the trophectoderm.
Journal ArticleDOI
Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro.
TL;DR: The derivation of pluripotent embryonic stem (ES) cells from human blastocysts is described, providing a model to study early human embryology, an investigational tool for discovery of novel growth factors and medicines, and a potential source of cells for use in transplantation therapy.
Journal ArticleDOI
Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers.
Joseph Itskovitz-Eldor,Maya Schuldiner,Dorit Karsenti,Amir Eden,Ofra Yanuka,Michal Amit,Hermona Soreq,Nissim Benvenisty +7 more
TL;DR: The ability to induce formation of human embryoid bodies that contain cells of neuronal, hematopoietic and cardiac origins will be useful in studying early human embryonic development as well as in transplantation medicine.