Journal ArticleDOI
Derivation of embryonic stem-cell lines from human blastocysts.
Chad A. Cowan,Irinha Klimanskaya,Jill A. McMahon,Jocelyn Atienza,Jeannine Witmyer,Jacob P. Zucker,Shunping Wang,Cynthia C. Morton,Andrew P. McMahon,Doug Powers,Douglas A. Melton +10 more
TLDR
The procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts are discussed.Abstract:
This report, first published online on March 3, 2004, discusses the procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts. These cell lines are available to researchers under a Material Transfer Agreement; according to current regulations, the cells cannot be used for research supported by federal funds. These cells are expected to facilitate research on a variety of serious chronic diseases.read more
Citations
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Derivation of human embryonic stem cells from day-8 blastocysts recovered after three-step in vitro culture.
Miodrag Stojkovic,Majlinda Lako,Petra Stojkovic,Rebecca Stewart,Stefan Przyborski,Lyle Armstrong,Jerry Evans,Mary Herbert,Louise A. Hyslop,Sajjad Ahmad,Alison Murdoch,Tom Strachan +11 more
TL;DR: It is reported here that the novel three‐step culture conditions successfully support the development of day‐8 human blastocysts, which possess significantly (p <.01) more ICM cells than day‐6 Blastocysts.
Journal ArticleDOI
Enhanced efficiency of generating induced pluripotent stem (iPS) cells from human somatic cells by a combination of six transcription factors.
Jing Liao,Zhao Wu,Ying Wang,Lu Cheng,Chun Cui,Yuan Gao,Taotao Chen,Lingjun Rao,Siye Chen,Nannan Jia,Huiming Dai,Shunmei Xin,Jiuhong Kang,Gang Pei,Lei Xiao +14 more
TL;DR: Enhanced efficiency of generating induced pluripotent stem cells from human somatic cells by a combination of six transcription factors is demonstrated.
Journal ArticleDOI
Generation of hypoimmunogenic human pluripotent stem cells
Xiao Han,Mengning Wang,Songwei Duan,Paul J. Franco,Jennifer H. R. Kenty,Preston Hedrick,Yulei Xia,Alana Allen,Leonardo M. R. Ferreira,Jack L. Strominger,Douglas A. Melton,Torsten B. Meissner,Torsten B. Meissner,Chad A. Cowan +13 more
TL;DR: An approach is described that effectively targets adaptive as well as innate immune responses and may therefore enable cell therapy on a broader scale and informs future studies aiming to generate “off-the-shelf” universal cell products that may make cell therapy available to a larger pool of patients.
Journal ArticleDOI
Derivation, growth and applications of human embryonic stem cells
TL;DR: Recent progress in the derivation and growth of undifferentiated hES cells and their differentiated progeny, and the problems associated with these techniques are summarised.
Journal ArticleDOI
Derivation, characterization and differentiation of human embryonic stem cells: comparing serum-containing versus serum-free media and evidence of germ cell differentiation
Hsin Fu Chen,Hung-Chih Kuo,Hung-Chih Kuo,Chung-Liang Chien,Chia-Tung Shun,Yi-Lin Yao,Ip Pl,Ip Pl,Ching Yu Chuang,Wang Cc,Yu Shih Yang,Hong Nerng Ho +11 more
TL;DR: The data support that hESC may be capable of differentiation into germ cells although further confirmation is needed, and it is suggested that strategies such as stepwise adaptation will be needed before implementing a serum-free culture condition for h ESC lines that have previously been derived in a medium containing serum.
References
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Journal ArticleDOI
Embryonic Stem Cell Lines Derived from Human Blastocysts
James A. Thomson,Joseph Itskovitz-Eldor,Sander S. Shapiro,Michelle A. Waknitz,Swiergiel Jennifer J,Vivienne S. Marshall,Jeffrey M. Jones +6 more
TL;DR: Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages.
Journal ArticleDOI
The serial cultivation of human diploid cell strains.
Leonard Hayflick,P.S. Moorhead +1 more
TL;DR: A consideration of the cause of the eventual degeneration of these strains leads to the hypothesis that non-cumulative external factors are excluded and that the phenomenon is attributable to intrinsic factors which are expressed as senescence at the cellular level.
Journal ArticleDOI
Formation of Pluripotent Stem Cells in the Mammalian Embryo Depends on the POU Transcription Factor Oct4
Jennifer Nichols,Branko Zevnik,Konstantinos Anastassiadis,Hitoshi Niwa,Daniela Klewe-Nebenius,Ian Chambers,Hans R. Schöler,Austin Smith +7 more
TL;DR: It is reported that the activity of Oct4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo and also determines paracrine growth factor signaling from stem cells to the trophectoderm.
Journal ArticleDOI
Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro.
TL;DR: The derivation of pluripotent embryonic stem (ES) cells from human blastocysts is described, providing a model to study early human embryology, an investigational tool for discovery of novel growth factors and medicines, and a potential source of cells for use in transplantation therapy.
Journal ArticleDOI
Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers.
Joseph Itskovitz-Eldor,Maya Schuldiner,Dorit Karsenti,Amir Eden,Ofra Yanuka,Michal Amit,Hermona Soreq,Nissim Benvenisty +7 more
TL;DR: The ability to induce formation of human embryoid bodies that contain cells of neuronal, hematopoietic and cardiac origins will be useful in studying early human embryonic development as well as in transplantation medicine.