Journal ArticleDOI
Derivation of embryonic stem-cell lines from human blastocysts.
Chad A. Cowan,Irinha Klimanskaya,Jill A. McMahon,Jocelyn Atienza,Jeannine Witmyer,Jacob P. Zucker,Shunping Wang,Cynthia C. Morton,Andrew P. McMahon,Doug Powers,Douglas A. Melton +10 more
TLDR
The procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts are discussed.Abstract:
This report, first published online on March 3, 2004, discusses the procedures used to develop 17 lines of human embryonic stem cells from the inner cell masses of blastocysts. These cell lines are available to researchers under a Material Transfer Agreement; according to current regulations, the cells cannot be used for research supported by federal funds. These cells are expected to facilitate research on a variety of serious chronic diseases.read more
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Patent
Efficient method for nuclear reprogramming
Shinya Yamanaka,Michiyo Koyanagi +1 more
TL;DR: In this paper, a method of preparing induced pluripotent stem cells, comprising a nuclear reprogramming step with a nuclear re-programming factor in the presence of miRNA, was proposed.
Journal ArticleDOI
A 3D microfibrous scaffold for long-term human pluripotent stem cell self-renewal under chemically defined conditions.
TL;DR: An engineered 3D microfibrous system that efficiently supports long-term hPSCs self-renewal under chemically defined conditions has the potential for large-scale cultivation of transplantable hESCs and derivatives for clinical applications.
Journal ArticleDOI
Methods for Expansion of Human Embryonic Stem Cells
Sun Kyung Oh,Hee Sun Kim,Yong Bin Park,Hye Won Seol,Yoon Young Kim,Myung Soo Cho,Seung Yup Ku,Young Min Choi,Dong-Wook Kim,Shin Yong Moon +9 more
TL;DR: The combination of two methods allows mass production of hESCs by excluding differentiated colonies from passage by manual selection before enzyme treatment and implementing the enzymatic transfer method when the bulk production of cells is needed.
Journal ArticleDOI
Immortalized feeders for the scale-up of human embryonic stem cells in feeder and feeder-free conditions
TL;DR: This study immortalized a primary MEF line with the E6 and E7 genes from HPV16 and demonstrated the feasibility of scaling-up significant quantities of undifferentiated hESC (>10(8) cells) using DeltaE-MEF in cell factories.
Journal ArticleDOI
Characterization and hepatogenic differentiation of mesenchymal stem cells from human amniotic fluid and human bone marrow: A comparative study
TL;DR: Investigation of the in vitro biological characterization and hepatic differentiation potential of human amniotic fluid‐derived mesenchymal stem cells (AF‐hMSCs) and human bone marrow‐derived desorption and differentiation (BM‐h MSCs) shows that AF‐ hMSCs possess higher proliferation and self‐renewal capacity than BM‐hmsCs.
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Journal ArticleDOI
Embryonic Stem Cell Lines Derived from Human Blastocysts
James A. Thomson,Joseph Itskovitz-Eldor,Sander S. Shapiro,Michelle A. Waknitz,Swiergiel Jennifer J,Vivienne S. Marshall,Jeffrey M. Jones +6 more
TL;DR: Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages.
Journal ArticleDOI
The serial cultivation of human diploid cell strains.
Leonard Hayflick,P.S. Moorhead +1 more
TL;DR: A consideration of the cause of the eventual degeneration of these strains leads to the hypothesis that non-cumulative external factors are excluded and that the phenomenon is attributable to intrinsic factors which are expressed as senescence at the cellular level.
Journal ArticleDOI
Formation of Pluripotent Stem Cells in the Mammalian Embryo Depends on the POU Transcription Factor Oct4
Jennifer Nichols,Branko Zevnik,Konstantinos Anastassiadis,Hitoshi Niwa,Daniela Klewe-Nebenius,Ian Chambers,Hans R. Schöler,Austin Smith +7 more
TL;DR: It is reported that the activity of Oct4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo and also determines paracrine growth factor signaling from stem cells to the trophectoderm.
Journal ArticleDOI
Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro.
TL;DR: The derivation of pluripotent embryonic stem (ES) cells from human blastocysts is described, providing a model to study early human embryology, an investigational tool for discovery of novel growth factors and medicines, and a potential source of cells for use in transplantation therapy.
Journal ArticleDOI
Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers.
Joseph Itskovitz-Eldor,Maya Schuldiner,Dorit Karsenti,Amir Eden,Ofra Yanuka,Michal Amit,Hermona Soreq,Nissim Benvenisty +7 more
TL;DR: The ability to induce formation of human embryoid bodies that contain cells of neuronal, hematopoietic and cardiac origins will be useful in studying early human embryonic development as well as in transplantation medicine.