Journal ArticleDOI
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
M. Dagert,S.D. Ehrlich +1 more
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Escherichia coli cells are 4--6 times more transformable and 20--30 times more competent after 24 h incubation in cold calcium chloride than immediately after calcium chloride treatment.About:
This article is published in Gene.The article was published on 1979-01-01. It has received 1354 citations till now. The article focuses on the topics: Calcium & Incubation.read more
Citations
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Bioactive recombinant methionyl bovine prolactin: structure-function studies using site-specific mutagenesis.
TL;DR: It appears that the first putative alpha-helix of bPRL is important for the binding and mitogenic activity of the hormone.
Journal ArticleDOI
Overproduction of the EcoR V endonuclease and methylase.
TL;DR: The strains described here allowed a quick purification of both enzymes in sufficient amounts for crystallisation attempts, and the situation in the endonuclease overproducing clone resembles that encountered in maxi-cells.
Journal ArticleDOI
Secretory expression in Escherichia coli and Bacillus subtilis of human interferon α genes directed by staphylokinase signals
TL;DR: A DNA segment covering the signal sequence coding region, the ribosome binding site, and the promoter of the staphylokinase (sak) 42D gene was cloned into pUC19 to form a portable expression-secretion unit (ESU) that resulted in secretory expression of the two gene products in both Escherichia coli and Bacillus subtilis.
Journal ArticleDOI
UDP-N-acetylglucosamine:N-acetylmuramoyl-(pentapeptide) pyrophosphoryl undecaprenol N-acetylglucosamine transferase from Escherichia coli: overproduction, solubilization, and purification.
TL;DR: Plasmids for the high‐level overproduction of wild‐type, and C‐ and N‐terminal His‐tagged MurG N‐acetylglucosaminyl transferase from Escherichia coli were constructed and in complementation tests the three forms were active in vivo.
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Characterization of CidR‐mediated regulation in Bacillus anthracis reveals a previously undetected role of S‐layer proteins as murein hydrolases
Jong Sam Ahn,Jong Sam Ahn,Lakshmi Chandramohan,Lakshmi Chandramohan,Linda E. Liou,Kenneth W. Bayles +5 more
TL;DR: The results of these studies not only establish the existence of the cid and lrg murein hydrolase regulatory network in B. anthracis, but also help to define the function and regulation of the S‐layer proteins.
References
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Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Francisco Bolívar,Raymond L. Rodriguez,Patricia J. Greene,Mary C. Betlach,Herbert L. Heyneker,Herbert W. Boyer,Jorge H. Crosa,Stanley Falkow +7 more
TL;DR: In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).
Journal ArticleDOI
Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNA
TL;DR: Covalently-closed, catenated, and open (nicked) circular forms of R-factor DNA are all effective in transformation, but denaturation and sonication abolish the transforming ability of R.factor DNA in this system.
Journal ArticleDOI
Calcium-dependent bacteriophage DNA infection.
Manley Mandel,A. Higa +1 more
TL;DR: Escherichia coli cells of strain K12 and C can be made competent to take up temperate phage DNA without the use of “helper phage”, and is effective for both linear and circular DNA molecules.