Journal ArticleDOI
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
M. Dagert,S.D. Ehrlich +1 more
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Escherichia coli cells are 4--6 times more transformable and 20--30 times more competent after 24 h incubation in cold calcium chloride than immediately after calcium chloride treatment.About:
This article is published in Gene.The article was published on 1979-01-01. It has received 1354 citations till now. The article focuses on the topics: Calcium & Incubation.read more
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Book ChapterDOI
Shuttle mutagenesis: bacterial transposons for genetic manipulations in yeast.
TL;DR: This chapter describes the methods and application of the Tn3 shuttle mutagenesis system, which can be used for random insertions scattered throughout the genome and used to screen for specific phenotypes linked to the insertion mutations.
Journal ArticleDOI
Presence in the 'silent' terminus region of the Escherichia coli K12 chromosome of cryptic gene(s) encoding a new nitrate reductase.
TL;DR: It is proposed that narZ and narGHI have descended from a common ancestor by gene duplication, and a large extent of homology, with a small area of very high homology.
Journal ArticleDOI
Sequence and expression of the Escherichia coli K1 neuC gene product.
TL;DR: A chimeric protein with beta-galactosidase fused to the carboxy terminus of the neuC gene product was constructed and purified, and its amino-terminal sequence confirmed the prediction from the nucleotide sequence that the neUC gene overlaps the distal end of the NeuA gene by a single base pair.
Journal ArticleDOI
Identification of variable region differences in Neisseria meningitidis class 3 protein sequences among five group B serotypes.
TL;DR: Two major variable regions in the translated protein sequence, VR1 and VR2, that may be associated with serotype specificity are identified and are predicted to be in surface‐exposed loops.
Journal ArticleDOI
Cloning of the adenylate cyclase genetic determinant of Bordetella pertussis and its expression in Escherichia coli and B. pertussis.
TL;DR: A recombinant plasmid, pRMB1, identified from a gene library of B. pertussis, restored adenylate cyclase (AC) and haemolysin (HLY) activities to B.pertussis BP348 (a Tn5-insertion mutant deficient in both these activities).
References
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Journal ArticleDOI
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Francisco Bolívar,Raymond L. Rodriguez,Patricia J. Greene,Mary C. Betlach,Herbert L. Heyneker,Herbert W. Boyer,Jorge H. Crosa,Stanley Falkow +7 more
TL;DR: In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).
Journal ArticleDOI
Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNA
TL;DR: Covalently-closed, catenated, and open (nicked) circular forms of R-factor DNA are all effective in transformation, but denaturation and sonication abolish the transforming ability of R.factor DNA in this system.
Journal ArticleDOI
Calcium-dependent bacteriophage DNA infection.
Manley Mandel,A. Higa +1 more
TL;DR: Escherichia coli cells of strain K12 and C can be made competent to take up temperate phage DNA without the use of “helper phage”, and is effective for both linear and circular DNA molecules.