Journal ArticleDOI
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
M. Dagert,S.D. Ehrlich +1 more
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Escherichia coli cells are 4--6 times more transformable and 20--30 times more competent after 24 h incubation in cold calcium chloride than immediately after calcium chloride treatment.About:
This article is published in Gene.The article was published on 1979-01-01. It has received 1354 citations till now. The article focuses on the topics: Calcium & Incubation.read more
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Molecular cloning and regulatory analysis of the arylsulfatase structural gene of Neurospora crassa.
TL;DR: Time course analysis showed that the appearance of ars-1+ message in sulfur-derepressed cultures paralleled the appearanceof arylsulfatase enzyme activity, and transcription of arS-1- was detected only under derepressing conditions in a nuclear transcription assay, in a cys-3 regulatory mutant that was unable to synthesize arylulfatases (or other sulfur-controlled enzymes).
Journal ArticleDOI
Efficient selection of hygromycin-B-resistant Yarrowia lipolytica transformants.
TL;DR: Direct selection of hygromycin-B-resistant transformants on complete medium was very efficient and resulted in transformation frequencies comparable to those observed with conventional auxotrophic markers, which can be used for integrating single copies of plasmid and for gene disruption.
Journal ArticleDOI
Lamprey fibrinogen gamma chain: cloning, cDNA sequencing, and general characterization.
D. D. Strong,Moore M,Barbara A. Cottrell,V. L. Bohonus,Manuel C. F. Pontes,Brian R. Evans,Marcia Riley,Russell F. Doolittle +7 more
TL;DR: The amino acid sequence of the fibrinogen gamma chain has been differentially conserved during evolution, the lamprey and human sequences being more than 70% identical in certain key regions but dropping to less than 25% in other sections, including the segment thought to be a part of the "coiled coils".
Journal ArticleDOI
Relationship between promoter structure and template specificities exhibited by the bacteriophage T3 and T7 RNA polymerases
TL;DR: To explore the basis for the template specificities of the bacteriophage T3 and T7 RNA polymerases (EC 2.7.6), nucleotide sequences of six promoters recognized by the T3 RNA polymerase were determined and compared with the previously determined promoter sequences recognized byThe bacteriophile T7RNA polymerase.
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Rous sarcoma virus variants that encode src proteins with an altered carboxy terminus are defective for cellular transformation.
TL;DR: Data indicate that the carboxy-terminal amino acid sequences play an important role in maintaining the structure of the catalytic domain of pp60v-src, and the transfection of chicken cells with plasmid DNA containing a chimeric v-c-src gene resulted in morphological cell transformation and the synthesis of an enzymatically active hybrid protein.
References
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Journal ArticleDOI
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Francisco Bolívar,Raymond L. Rodriguez,Patricia J. Greene,Mary C. Betlach,Herbert L. Heyneker,Herbert W. Boyer,Jorge H. Crosa,Stanley Falkow +7 more
TL;DR: In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).
Journal ArticleDOI
Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNA
TL;DR: Covalently-closed, catenated, and open (nicked) circular forms of R-factor DNA are all effective in transformation, but denaturation and sonication abolish the transforming ability of R.factor DNA in this system.
Journal ArticleDOI
Calcium-dependent bacteriophage DNA infection.
Manley Mandel,A. Higa +1 more
TL;DR: Escherichia coli cells of strain K12 and C can be made competent to take up temperate phage DNA without the use of “helper phage”, and is effective for both linear and circular DNA molecules.