Journal ArticleDOI
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
M. Dagert,S.D. Ehrlich +1 more
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Escherichia coli cells are 4--6 times more transformable and 20--30 times more competent after 24 h incubation in cold calcium chloride than immediately after calcium chloride treatment.About:
This article is published in Gene.The article was published on 1979-01-01. It has received 1354 citations till now. The article focuses on the topics: Calcium & Incubation.read more
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Identification and characterization of insertion sequence ISRm1 in Rhizobium meliloti JJ1c10
TL;DR: ISRm1, an insertion sequence present in Rhizobium meliloti strain 1021, has been identified as the cause of the Nod− phenotypes in two mutants of another strain, JJ1c10.
Journal ArticleDOI
Expression of FBJ-MSV oncogene (fos) product in bacteria
TL;DR: The protein predicted from the DNA sequence of the FBJ murine osteosarcoma virus (FBJ-MSV) onc gene (v-fos) was expressed in Escherichia coli under the control of the tryptophan operon regulatory region.
Journal ArticleDOI
Effect of growth rate and nutrient limitation on the transformability of Escherichia coli with plasmid deoxyribonucleic acid.
TL;DR: The observed transformation frequency by plasmid deoxyribonucleic acid of Escherichia coli grown in continuous culture was found to depend on both the steady-state growth rate and the type of nutrient used to limit growth.
Journal ArticleDOI
Cloning and expression of the 3‐isopropylmalate dehydrogenase gene from Candida albicans
TL;DR: The cloning of a fragment of C. albicans DNA carrying the gene for 3-IMDH which will be useful in the development of transformation methods in C.Albicans is described.
Journal ArticleDOI
Rapid evolution of variants in a rodent multigene family encoding salivary proteins.
TL;DR: The pattern of amino acid replacements and nucleotide substitutions is consistent with little, if any, selection constraint on the precise sequence of the spot proteins, suggesting that it is the overall architecture of the molecule, rather than the precise structure, that is important for function.
References
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Journal ArticleDOI
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Francisco Bolívar,Raymond L. Rodriguez,Patricia J. Greene,Mary C. Betlach,Herbert L. Heyneker,Herbert W. Boyer,Jorge H. Crosa,Stanley Falkow +7 more
TL;DR: In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).
Journal ArticleDOI
Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNA
TL;DR: Covalently-closed, catenated, and open (nicked) circular forms of R-factor DNA are all effective in transformation, but denaturation and sonication abolish the transforming ability of R.factor DNA in this system.
Journal ArticleDOI
Calcium-dependent bacteriophage DNA infection.
Manley Mandel,A. Higa +1 more
TL;DR: Escherichia coli cells of strain K12 and C can be made competent to take up temperate phage DNA without the use of “helper phage”, and is effective for both linear and circular DNA molecules.