Journal ArticleDOI
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
M. Dagert,S.D. Ehrlich +1 more
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Escherichia coli cells are 4--6 times more transformable and 20--30 times more competent after 24 h incubation in cold calcium chloride than immediately after calcium chloride treatment.About:
This article is published in Gene.The article was published on 1979-01-01. It has received 1354 citations till now. The article focuses on the topics: Calcium & Incubation.read more
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Clone banks of the mung bean, pea and spinach chloroplast genomes
TL;DR: All but one of the PstI restriction fragments from mung bean, pea, and spinach chloroplast DNAs have been stably cloned into pBR322 and the identity of the cloned fragments to nativechloroplast DNA restriction fragments is demonstrated by restriction analysis and the ability to construct detailed restriction maps of the mungbean and pea chloropleft genomes.
Journal ArticleDOI
The consequences of stepwise deletions from the signal-processing site of beta-lactamase
A Plückthun,J R Knowles +1 more
TL;DR: The results illustrate the limits on the amino acid sequence around the processing junction and suggest that interference with the signal cleavage step can lead not only to aborted secretion but also to pleiotropic consequences for the growth of the host organism.
Journal ArticleDOI
Characterization of the pColV‐K30 encoded cloacin DF13/aerobactin outer membrane receptor protein of Escherichia coli; isolation and purification of the protein and analysis of its nucleotide sequence and primary structure
W. J. A. Krone,F Stegehuis,Gregory Koningstein,Carla van Doorn,B. Roosendaal,Frits K. de Graaf,Bauke Oudega +6 more
TL;DR: The results revealed that the pColV-K30-specified receptor protein might be synthesized as a precursor, with a signal sequence of 25 amino acid residues, and the mature protein has an M r of 77 345.
Journal ArticleDOI
Genetic Environment and Transcription of ampC in an Acinetobacter baumannii Clinical Isolate
Heidi Segal,E. C. Nelson +1 more
TL;DR: DNA sequencing and primer extension studies showed that ampC is transcribed from a promoter contained within a putative insertion sequence element which has been found to abut several different genes in Acinetobacter spp.
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Localization of a symbiotic fix region on Rhizobium meliloti pSym megaplasmid more than 200 kilobases from the nod-nif region
Jacques Batut,B Terzaghi,M. Gherardi,M. Huguet,E. Terzaghi,A. M. Garnerone,P. Boistard,Thierry Huguet +7 more
TL;DR: The HindIII physical map of a cloned 290 kilobase fragment of the Rhizobium meliloti 2011 pSym megaplasmid has been established and a region which is located more than 200 kb from the nifHDK operon on pSym is essential for symbiotic nitrogen fixation.
References
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Journal ArticleDOI
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Francisco Bolívar,Raymond L. Rodriguez,Patricia J. Greene,Mary C. Betlach,Herbert L. Heyneker,Herbert W. Boyer,Jorge H. Crosa,Stanley Falkow +7 more
TL;DR: In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).
Journal ArticleDOI
Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNA
TL;DR: Covalently-closed, catenated, and open (nicked) circular forms of R-factor DNA are all effective in transformation, but denaturation and sonication abolish the transforming ability of R.factor DNA in this system.
Journal ArticleDOI
Calcium-dependent bacteriophage DNA infection.
Manley Mandel,A. Higa +1 more
TL;DR: Escherichia coli cells of strain K12 and C can be made competent to take up temperate phage DNA without the use of “helper phage”, and is effective for both linear and circular DNA molecules.