Journal ArticleDOI
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
M. Dagert,S.D. Ehrlich +1 more
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Escherichia coli cells are 4--6 times more transformable and 20--30 times more competent after 24 h incubation in cold calcium chloride than immediately after calcium chloride treatment.About:
This article is published in Gene.The article was published on 1979-01-01. It has received 1354 citations till now. The article focuses on the topics: Calcium & Incubation.read more
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Sequence and analysis of the capsid protein of Nudaurelia capensis ω virus, an insect virus with T = 4 icosahedral symmetry
Deepak Agrawal,John E. Johnson +1 more
TL;DR: The nucleotide sequence of the RNA2 segment of the Nudaurelia capensis omega virus genome was found to consist of 2448 nucleotides and contained one long open reading frame (ORF) encoding the 644 residue capsid protein.
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Bacteriophage T4 DNA replication protein 41. Cloning of the gene and purification of the expressed protein.
TL;DR: The cloning of T4 DNA representing map units 24.3 to 20.06 in a multicopy plasmid vector results in the production of active 41 protein at a level 100-fold greater than after T4 infection.
Journal ArticleDOI
The translational efficiency of tRNA is a property of the anticodon arm.
TL;DR: The most efficient sequences in this series resemble those found in natural tRNAs associated with similar anticodons, as is proposed in the extended anticodon theory, and provide some information on the specificity of nucleotide modification enzymes and on determinants of the steady-state tRNA level.
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Strict co-linearity of genetic and protein folding domains in an intragenically duplicated rat lens γ-crystallin gene
R. J. M. Moormann,Johan T. den Dunnen,Leon Mulleners,Peter M. Andreoli,Hans Bloemendal,John G.G. Schoenmakers +5 more
TL;DR: Although expected from the cDNA and protein sequence no introns were observed between the coding regions in the DNA specifying the two homologous folding motifs present in each protein domain, the relevance of this phenomenon in terms of the evolution of the mature gamma-crystallin gene is discussed.
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Invertase gene (SUC2) of Saccharomyces cerevisiae as a dominant marker for transformation of Pichia pastoris.
TL;DR: It was shown that the Suc+ transformants of P. pastoris with the autonomously replicating plasmid were highly unstable with respect to thePlasmid maintenance, even when grown on sucrose as the sole carbon and energy source, despite being attributed to an efficient cross-feeding by Suc- segregants on glucose and fructose generated due to hydrolysis of sucrose by the invertase enzyme secreted by Suc+ cells.
References
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Journal ArticleDOI
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Francisco Bolívar,Raymond L. Rodriguez,Patricia J. Greene,Mary C. Betlach,Herbert L. Heyneker,Herbert W. Boyer,Jorge H. Crosa,Stanley Falkow +7 more
TL;DR: In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).
Journal ArticleDOI
Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNA
TL;DR: Covalently-closed, catenated, and open (nicked) circular forms of R-factor DNA are all effective in transformation, but denaturation and sonication abolish the transforming ability of R.factor DNA in this system.
Journal ArticleDOI
Calcium-dependent bacteriophage DNA infection.
Manley Mandel,A. Higa +1 more
TL;DR: Escherichia coli cells of strain K12 and C can be made competent to take up temperate phage DNA without the use of “helper phage”, and is effective for both linear and circular DNA molecules.