Journal ArticleDOI
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
M. Dagert,S.D. Ehrlich +1 more
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Escherichia coli cells are 4--6 times more transformable and 20--30 times more competent after 24 h incubation in cold calcium chloride than immediately after calcium chloride treatment.About:
This article is published in Gene.The article was published on 1979-01-01. It has received 1354 citations till now. The article focuses on the topics: Calcium & Incubation.read more
Citations
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Journal ArticleDOI
Characterization of antibiotic resistance in commensal bacteria from an aquaculture ecosystem
TL;DR: The data indicated a need to update the methodology due to genetic diversity of microbiota for better understanding of the AR ecology, and suggested AR-rich feed and possibly environmental factors may contribute to AR in the aquaculture ecosystem.
Book ChapterDOI
Beta 2-microglobulin
Ettore Appella,Janet A. Sawicki +1 more
TL;DR: It has been demonstrated that β 2 M is in close association with several cell surface glycoproteins and is homologous in amino acid sequence to the sequences of the constant regions of both light and heavy chains of immunoglobulins.
Journal ArticleDOI
Transformation in Restriction-deficient Salmonella typhimurium LT2
TL;DR: Stable restriction-deficient, modification-proficient galE and F'galE+ strains of Salmonella typhimurium strains were constructed and the effects of restriction on transformation by plasmid pBR322 were tested.
Journal ArticleDOI
Long-chain fatty acid transport in Escherichia coli. Cloning, mapping, and expression of the fadL gene.
TL;DR: Whether the fadL gene is the structural gene for this 33,000-dalton protein is determined and a heat-modifiable protein which has an apparent molecular weight of 43,000 daltons when solubilized at 100 degrees C in the presence of SDS is described.
Journal ArticleDOI
Repeat and single copy sequences are differentially conserved in the evolution of kelp chloroplast DNA.
TL;DR: A method for the isolation of total blade, chloroplast and nuclear DNAs from kelp is described and total blade DNA was isolated from various kelp species at yields of about 1.0 μg.g−1 wet weight of tissue extracted.
References
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Journal ArticleDOI
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Francisco Bolívar,Raymond L. Rodriguez,Patricia J. Greene,Mary C. Betlach,Herbert L. Heyneker,Herbert W. Boyer,Jorge H. Crosa,Stanley Falkow +7 more
TL;DR: In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).
Journal ArticleDOI
Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNA
TL;DR: Covalently-closed, catenated, and open (nicked) circular forms of R-factor DNA are all effective in transformation, but denaturation and sonication abolish the transforming ability of R.factor DNA in this system.
Journal ArticleDOI
Calcium-dependent bacteriophage DNA infection.
Manley Mandel,A. Higa +1 more
TL;DR: Escherichia coli cells of strain K12 and C can be made competent to take up temperate phage DNA without the use of “helper phage”, and is effective for both linear and circular DNA molecules.