Journal ArticleDOI
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
M. Dagert,S.D. Ehrlich +1 more
Reads0
Chats0
TLDR
Escherichia coli cells are 4--6 times more transformable and 20--30 times more competent after 24 h incubation in cold calcium chloride than immediately after calcium chloride treatment.About:
This article is published in Gene.The article was published on 1979-01-01. It has received 1354 citations till now. The article focuses on the topics: Calcium & Incubation.read more
Citations
More filters
Journal ArticleDOI
Plasmid replication stimulates DNA recombination in Bacillus subtilis.
TL;DR: Results show that plasmid replication stimulates DNA recombination.
Journal ArticleDOI
A binding-lipoprotein-dependent oligopeptide transport system in Streptococcus gordonii essential for uptake of hexa- and heptapeptides.
TL;DR: The results suggest that the formation of a solute-binding-protein complex consisting of at least the HppA and theHppH lipopolypeptides is necessary for binding and subsequent uptake of primarily hexa- or heptapeptides by a Hpp (Hexa-heptAPEptide permease) system in S. gordonii.
Journal ArticleDOI
Integrated DNA sequences in three streptomycetes form related autonomous plasmids after transfer to Streptomyces lividans
TL;DR: In this article, the authors derived restriction maps of pIJ110 and pIJ408, each for 10 enzymes, and compared them with the previously known SLP1 plasmids (SLP1.2) which come from integrated sequences in the chromosome of S. coelicolor A3(2).
Journal ArticleDOI
Identification and characterization of three new promoter/operators from Corynebacterium diphtheriae that are regulated by the diphtheria toxin repressor (DtxR) and iron.
TL;DR: Three additional DtxR-regulated promoter/operators from C. diphtheriae designated IRP3, IRP4, and IRP5 are identified and characterized and polypeptide products of the predicted sizes were detected in small amounts by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
Journal ArticleDOI
Cloning and mapping of the genetic determinants for microcin C7 production and immunity.
TL;DR: Analysis of plasmids carrying mcc-lacZ gene fusions indicates that all microcin DNA is transcribed in the same direction, suggesting that a structure like a polycistronic operon is responsible for microcin C7 production and immunity.
References
More filters
Journal ArticleDOI
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Francisco Bolívar,Raymond L. Rodriguez,Patricia J. Greene,Mary C. Betlach,Herbert L. Heyneker,Herbert W. Boyer,Jorge H. Crosa,Stanley Falkow +7 more
TL;DR: In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).
Journal ArticleDOI
Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNA
TL;DR: Covalently-closed, catenated, and open (nicked) circular forms of R-factor DNA are all effective in transformation, but denaturation and sonication abolish the transforming ability of R.factor DNA in this system.
Journal ArticleDOI
Calcium-dependent bacteriophage DNA infection.
Manley Mandel,A. Higa +1 more
TL;DR: Escherichia coli cells of strain K12 and C can be made competent to take up temperate phage DNA without the use of “helper phage”, and is effective for both linear and circular DNA molecules.