Journal ArticleDOI
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
M. Dagert,S.D. Ehrlich +1 more
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Escherichia coli cells are 4--6 times more transformable and 20--30 times more competent after 24 h incubation in cold calcium chloride than immediately after calcium chloride treatment.About:
This article is published in Gene.The article was published on 1979-01-01. It has received 1354 citations till now. The article focuses on the topics: Calcium & Incubation.read more
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The murI gene of Escherichia coli is an essential gene that encodes a glutamate racemase activity.
TL;DR: Experiments of insertional mutagenesis of the murI gene are reported which demonstrate that this gene is essential for the biosynthesis of D-glutamic acid, one of the specific components of cell wall peptidoglycan.
Journal ArticleDOI
The Helicobacter pylori ureC gene codes for a phosphoglucosamine mutase.
TL;DR: It is demonstrated that complete complementation of the glmM mutant can be obtained with a plasmid overproducing UreC, and significant conservation of the amino acid sequence of these proteins in such diverse organisms suggests a very ancient common ancestor for the genes.
Journal ArticleDOI
Regulation of expression of Streptococcus mutans genes important to virulence.
M C Hudson,rd R Curtiss +1 more
TL;DR: Studies were initiated to investigate the regulation of Streptococcus mutans genes which are believed to be important to virulence and found that expression of these genes is increased by the presence of sucrose and is followed by a rapid decline in expression over time.
Journal ArticleDOI
Identification of functional regions in the transforming protein of Fujinami sarcoma virus by in-phase insertion mutagenesis
TL;DR: Results imply that both the NH2- and COOH-terminal fps-specific portions of the FSV oncogene product possess determinants which function in fibroblast transformation, and that cooperation of these two regions is not sensitive to their separation in the primary structure.
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Primary sequence of the Escherichia coli fadL gene encoding an outer membrane protein required for long-chain fatty acid transport.
TL;DR: The fadL gene of Escherichia coli encodes an outer membrane protein (FadL) that plays a central role in the uptake of exogenous long-chain fatty acids and the amino acid composition of FadL deduced from the DNA sequence suggested that this protein contained an abundance of hydrophobic amino acid residues and lacked cysteinyl residues.
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Journal ArticleDOI
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Francisco Bolívar,Raymond L. Rodriguez,Patricia J. Greene,Mary C. Betlach,Herbert L. Heyneker,Herbert W. Boyer,Jorge H. Crosa,Stanley Falkow +7 more
TL;DR: In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).
Journal ArticleDOI
Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNA
TL;DR: Covalently-closed, catenated, and open (nicked) circular forms of R-factor DNA are all effective in transformation, but denaturation and sonication abolish the transforming ability of R.factor DNA in this system.
Journal ArticleDOI
Calcium-dependent bacteriophage DNA infection.
Manley Mandel,A. Higa +1 more
TL;DR: Escherichia coli cells of strain K12 and C can be made competent to take up temperate phage DNA without the use of “helper phage”, and is effective for both linear and circular DNA molecules.