Journal ArticleDOI
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
M. Dagert,S.D. Ehrlich +1 more
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Escherichia coli cells are 4--6 times more transformable and 20--30 times more competent after 24 h incubation in cold calcium chloride than immediately after calcium chloride treatment.About:
This article is published in Gene.The article was published on 1979-01-01. It has received 1354 citations till now. The article focuses on the topics: Calcium & Incubation.read more
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Role of the proregion in the production and secretion of the Yarrowia lipolytica alkaline extracellular protease.
TL;DR: It is proposed that the AEP prosequence plays an additional essential role in guiding the proper folding of the protein into a conformation compatible with secretion.
Journal ArticleDOI
Isolation and characterization of P1 minireplicons, lambda-P1:5R and lambda-P1:5L.
N Sternberg,S Austin +1 more
TL;DR: The results show that the relative copy numbers of exerting and responding elements are important for the incompatibility phenotype and strongly suggest that lambda-P1:5L plasmids lack a repressor of replication that can be supplied in trans from P1 but not from EcoRI fragment 5.
Journal ArticleDOI
A double layer plaque assay using spread plate technique for enumeration of bacteriophage MS2
Jiemin Cormier,Marlene E. Janes +1 more
TL;DR: The optimized plaque assay significantly improved visibility and consistency of the existing plaque assay methods and could be used in quantification of MS2.
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Structure and evolution of the bovine prothrombin gene.
TL;DR: The cloned bovine prothrombin gene has been characterized by partial DNA sequence analysis, including the 5' and 3' flanking sequences and all the intron-exon junctions, suggesting that introns have been inserted into these regions after the initial gene duplication events.
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Virulence genes are carried by a megaplasmid of the plant pathogen Pseudomonas solanacearum.
TL;DR: A class of avirulent mutants of the plant pathogenic bacterium Pseudomonas solanacearum, strain GMI1000, resistant to acridine orange, harbour a deletion of over 85 kb in their genome, suggesting that the majority of virulence genes identified so far are plasmid borne.
References
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Journal ArticleDOI
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Francisco Bolívar,Raymond L. Rodriguez,Patricia J. Greene,Mary C. Betlach,Herbert L. Heyneker,Herbert W. Boyer,Jorge H. Crosa,Stanley Falkow +7 more
TL;DR: In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).
Journal ArticleDOI
Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNA
TL;DR: Covalently-closed, catenated, and open (nicked) circular forms of R-factor DNA are all effective in transformation, but denaturation and sonication abolish the transforming ability of R.factor DNA in this system.
Journal ArticleDOI
Calcium-dependent bacteriophage DNA infection.
Manley Mandel,A. Higa +1 more
TL;DR: Escherichia coli cells of strain K12 and C can be made competent to take up temperate phage DNA without the use of “helper phage”, and is effective for both linear and circular DNA molecules.