Journal ArticleDOI
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
M. Dagert,S.D. Ehrlich +1 more
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Escherichia coli cells are 4--6 times more transformable and 20--30 times more competent after 24 h incubation in cold calcium chloride than immediately after calcium chloride treatment.About:
This article is published in Gene.The article was published on 1979-01-01. It has received 1354 citations till now. The article focuses on the topics: Calcium & Incubation.read more
Citations
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The plasmids of Acetobacter xylinum and their interaction with the host chromosome.
TL;DR: Results indicate that at least part of the genetic system of A. xylinum is unusual when compared to that of other genetically characterized bacteria.
Journal ArticleDOI
Identification of the protein encoded by rodC, a cell division gene from Bacillus subtilis.
TL;DR: This is the initial cloning of a cell division gene and the identification of its product from B. subtilis, and a 68 kiloDalton protein has been identified as the rodC gene product.
Journal ArticleDOI
Effect of a mutation preventing lipid modification on localization of the pCloDF13-encoded bacteriocin release protein and on release of cloacin DF13.
TL;DR: The results indicated that lipid modification of the BRP is required for its localization and release of cloacin DF13, but not for its lethality to host cells.
Journal ArticleDOI
Aspartate carbamoyltransferase from a psychrophilic deep-sea bacterium, Vibrio strain 2693 : properties of the enzyme, genetic organization and synthesis in Escherichia coli
Ying Xu,Yuanfu Zhang,Ziyuan Liang,Mark Van de Casteele,Christianne Legrain,Nicolas Glansdorff +5 more
TL;DR: Structural and functional comparisons indicate that Vibrio 2693 ATCase is a suitable model for biochemical studies on structure-function relationships in a 'cold' allosteric enzyme and strengthens the evolutionary relationship between Vibrionaceae and Enterobacteriaceae.
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Molecular analysis of the macrolide-lincosamide resistance gene region of a novel plasmid from Staphylococcus hyicus
TL;DR: The multiple point mutations shown in the pSES21-encoded ErmC translational attenuator represent a novel kind of structural alteration in this regulatory region and may explain constitutive ermC gene expression by pairing of the newly generated inverted repeated segments in the presence of a functionally deleted reading frame for the small regulatory peptide.
References
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Journal ArticleDOI
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Francisco Bolívar,Raymond L. Rodriguez,Patricia J. Greene,Mary C. Betlach,Herbert L. Heyneker,Herbert W. Boyer,Jorge H. Crosa,Stanley Falkow +7 more
TL;DR: In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).
Journal ArticleDOI
Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNA
TL;DR: Covalently-closed, catenated, and open (nicked) circular forms of R-factor DNA are all effective in transformation, but denaturation and sonication abolish the transforming ability of R.factor DNA in this system.
Journal ArticleDOI
Calcium-dependent bacteriophage DNA infection.
Manley Mandel,A. Higa +1 more
TL;DR: Escherichia coli cells of strain K12 and C can be made competent to take up temperate phage DNA without the use of “helper phage”, and is effective for both linear and circular DNA molecules.