Journal ArticleDOI
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
M. Dagert,S.D. Ehrlich +1 more
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Escherichia coli cells are 4--6 times more transformable and 20--30 times more competent after 24 h incubation in cold calcium chloride than immediately after calcium chloride treatment.About:
This article is published in Gene.The article was published on 1979-01-01. It has received 1354 citations till now. The article focuses on the topics: Calcium & Incubation.read more
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Patent
Method for tapping the immunological repertiore
TL;DR: In this article, a method for isolating from the immunological gene repertoire a gene coding for a receptor having the ability to bind a preselected ligand was proposed, and the effect of the gene isolated by the method was investigated.
Journal ArticleDOI
cDNA clone for the alpha-chain of human beta-hexosaminidase: deficiency of alpha-chain mRNA in Ashkenazi Tay-Sachs fibroblasts
TL;DR: A cDNA clone containing sequences complementary to mRNA encoding the alpha-chain of the lysosomal enzyme beta-hexosaminidase was isolated and hybridized to an mRNA of approximately equal to 1.9 kilobases; however, it was not seen in similar preparations from fibroblasts of four Ashkenazi Tay-Sachs patients.
Journal ArticleDOI
Role of the ortholog and paralog amino acid invariants in the active site of the UDP-MurNAc-L-alanine:D-glutamate ligase (MurD).
Ahmed Bouhss,Sébastien Dementin,Claudine Parquet,Dominique Mengin-Lecreulx,Bertrand Ja,Le Beller D,Dideberg O,van Heijenoort J,Didier Blanot +8 more
TL;DR: Examination of the specificity constants k(cat)/K(m) for the three MurD substrates indicated that most mutations affected both the binding of one substrate and the catalytic process, which correlated with the assigned function of the residues based on the X-ray structures.
Journal ArticleDOI
Interaction of type A lantibiotics with undecaprenol-bound cell envelope precursors.
TL;DR: It is shown that, besides binding to lipid II, nisin interacts with the lipid intermediates lipid III and lipid IV of the wall teichoic acid (WTA) biosynthesis pathway, which promoted target-mediated pore formation in artificial lipid bilayers.
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Evolution of transposable elements: an IS10 insertion increases fitness in Escherichia coli.
Lin Chao,S. M. Mcbroom +1 more
TL;DR: The results show that the IS10-generated insertion increases fitness in chemostat cultures and suggest that transposable elements may speed the rate of evolution by promoting nonhomologous recombination between preexisting variations within a genome and thereby generating adaptive variation.
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Journal ArticleDOI
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Francisco Bolívar,Raymond L. Rodriguez,Patricia J. Greene,Mary C. Betlach,Herbert L. Heyneker,Herbert W. Boyer,Jorge H. Crosa,Stanley Falkow +7 more
TL;DR: In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).
Journal ArticleDOI
Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNA
TL;DR: Covalently-closed, catenated, and open (nicked) circular forms of R-factor DNA are all effective in transformation, but denaturation and sonication abolish the transforming ability of R.factor DNA in this system.
Journal ArticleDOI
Calcium-dependent bacteriophage DNA infection.
Manley Mandel,A. Higa +1 more
TL;DR: Escherichia coli cells of strain K12 and C can be made competent to take up temperate phage DNA without the use of “helper phage”, and is effective for both linear and circular DNA molecules.