Journal ArticleDOI
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
M. Dagert,S.D. Ehrlich +1 more
Reads0
Chats0
TLDR
Escherichia coli cells are 4--6 times more transformable and 20--30 times more competent after 24 h incubation in cold calcium chloride than immediately after calcium chloride treatment.About:
This article is published in Gene.The article was published on 1979-01-01. It has received 1354 citations till now. The article focuses on the topics: Calcium & Incubation.read more
Citations
More filters
Journal ArticleDOI
Transcription regulation in thermophilic bacteria: High resolution contact probing of Bacillus stearothermophilus and Thermotoga neapolitana arginine repressor-operator interactions
Hui H. Song,Haifeng H. Wang,Daniel Gigot,Diliana Dimova,Vehary V. Sakanyan,Nicolas Glansdorff,Nicolas Glansdorff,Daniel Charlier +7 more
TL;DR: A high resolution contact map of the argC operator from Bacillus stearothermophilus and the argR operator from Thermotoga neapolitana is established, which reinforces the uniform view of arginine regulation, but also reveals a number of striking differences at particular positions of the boxes and in the length and base-pair composition of the spacer connecting two ARG boxes in the operator.
Journal ArticleDOI
Involvement of DNA superhelicity in minichromosome maintenance in Escherichia coli.
TL;DR: Evidence is presented that Escherichia coli minichromosomes are harbored at superhelical densities which are lower than those measured for other E. coli plasmids but are comparable to that of the chromosome.
Journal ArticleDOI
Characterization of a simian virus 40-transformed Fanconi anemia fibroblast cell line.
TL;DR: GM6914 should be a useful recipient cell line in experiments using DNA-mediated gene transfer to clone the normal allele of the gene which is defective in FA complementation group A, and would also be an excellent cell line for studies on mutagenesis, recombination and repair using plasmid vectors.
Book ChapterDOI
Molecular Cloning of Flavivirus Genomes for Comparative Analysis and Expression
Charles M. Rice,Lynn Dalgarno,Ricardo Galler,Young S. Hahn,Ellen G. Strauss,James H. Strauss +5 more
Journal ArticleDOI
Amylase and 16S rRNA genes from a hyperthermophilic archaebacterium.
TL;DR: A hyperthermophilic and amylolytic prokaryote, designated Rt3, was isolated from a thermal spring near Rotorua, New Zealand.
References
More filters
Journal ArticleDOI
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Francisco Bolívar,Raymond L. Rodriguez,Patricia J. Greene,Mary C. Betlach,Herbert L. Heyneker,Herbert W. Boyer,Jorge H. Crosa,Stanley Falkow +7 more
TL;DR: In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).
Journal ArticleDOI
Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNA
TL;DR: Covalently-closed, catenated, and open (nicked) circular forms of R-factor DNA are all effective in transformation, but denaturation and sonication abolish the transforming ability of R.factor DNA in this system.
Journal ArticleDOI
Calcium-dependent bacteriophage DNA infection.
Manley Mandel,A. Higa +1 more
TL;DR: Escherichia coli cells of strain K12 and C can be made competent to take up temperate phage DNA without the use of “helper phage”, and is effective for both linear and circular DNA molecules.