Journal ArticleDOI
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
M. Dagert,S.D. Ehrlich +1 more
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Escherichia coli cells are 4--6 times more transformable and 20--30 times more competent after 24 h incubation in cold calcium chloride than immediately after calcium chloride treatment.About:
This article is published in Gene.The article was published on 1979-01-01. It has received 1354 citations till now. The article focuses on the topics: Calcium & Incubation.read more
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Nitrous oxide reductase from denitrifying Pseudomonas stutzeri. Genes for copper-processing and properties of the deduced products, including a new member of the family of ATP/GTP-binding proteins.
TL;DR: The results provide evidence for an auxiliary system of Cu processing and suggest its involvement in the periplasmic biosynthesis of the Cu centers of N2O reductase.
Journal ArticleDOI
Nucleotide Sequence of Tomato Black Ring Virus RNA-1
C. Greif,Odile Hemmer,C. Fritsch +2 more
TL;DR: It is suggested that the genome organization of TBRV is similar to that of comoviruses and picornaviruses.
Journal ArticleDOI
The nucleotide sequence of the gene encoding the K99 subunit of enterotoxigenic Escherichia coli
TL;DR: The nucleotide sequence of the gene encoding the K99 fimbrial subunit of enterotoxigenic Escherichia coli was determined and it appeared that the subunit is composed of 159 amino acid residues preceded by a N-terminal signal sequence.
Journal ArticleDOI
Preparation of a DNA gene probe for detection of mercury resistance genes in gram-negative bacterial communities
TL;DR: A DNA gene probe constructed from a 2.6-kilobase NcoI-EcoRI DNA restriction fragment can be utilized to study gene flow processes in gram-negative bacterial communities and no such homologies were detected in DNA of the gram-positive strains.
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Nucleotide sequence at the 3' terminus of pepper mottle virus genomic RNA: evidence for an alternative mode of potyvirus capsid protein gene organization
William G. Dougherty,Richard F. Allison,T. Dawn Parks,Robert E. Johnston,Mark J. Feild,Frank B. Armstrong +5 more
TL;DR: Combined nucleotide and amino acid sequence data suggest that the PeMV capsid protein is encoded by the 3'-most cistron on the genomic RNA and that it may be expressed as a precursor that is proteolytically processed to produce the mature capsidprotein.
References
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Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Francisco Bolívar,Raymond L. Rodriguez,Patricia J. Greene,Mary C. Betlach,Herbert L. Heyneker,Herbert W. Boyer,Jorge H. Crosa,Stanley Falkow +7 more
TL;DR: In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).
Journal ArticleDOI
Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNA
TL;DR: Covalently-closed, catenated, and open (nicked) circular forms of R-factor DNA are all effective in transformation, but denaturation and sonication abolish the transforming ability of R.factor DNA in this system.
Journal ArticleDOI
Calcium-dependent bacteriophage DNA infection.
Manley Mandel,A. Higa +1 more
TL;DR: Escherichia coli cells of strain K12 and C can be made competent to take up temperate phage DNA without the use of “helper phage”, and is effective for both linear and circular DNA molecules.