Journal ArticleDOI
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
M. Dagert,S.D. Ehrlich +1 more
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Escherichia coli cells are 4--6 times more transformable and 20--30 times more competent after 24 h incubation in cold calcium chloride than immediately after calcium chloride treatment.About:
This article is published in Gene.The article was published on 1979-01-01. It has received 1354 citations till now. The article focuses on the topics: Calcium & Incubation.read more
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Book ChapterDOI
[17] Oligonucleotide-directed site-specific mutagenesis of the 1ac permease of Escherichia coli
Hemanta K. Sarkar,Paul V. Viitanen,Etana Padan,William R. Trumble,Mohindar S. Poonian,Warren W. McComas,H. Ronald Kaback +6 more
TL;DR: It is illustrated that with the recent advent of site-directed mutagenesis, the goal of determining the role of amino acid residues or sequences in the function of a particular protein or enzyme, can be realized.
Journal ArticleDOI
The role of fimbriae of uropathogenic Escherichia coli as carriers of the adhesin involved in mannose-resistant hemagglutination.
TL;DR: The results indicate that adhesion and presence of fimbriae are not necessarily linked and P-fimbRIae could function as a carrier for the adhesin and thus endow adhesive capacity to cells with a complete O-antigen.
Journal ArticleDOI
Use of rRNA gene restriction patterns for the identification of Listeria species
TL;DR: Fifty-two representatives of the seven recognized species of the genus Listeria were characterized using ribosomal RNA gene polymorphism, a molecular classification system that makes it possible to allocate an isolate to a known taxon.
Journal ArticleDOI
Analysis and optimization of recombinant DNA joining reactions.
TL;DR: The statistical segment length of duplex DNA was determined by electron microscopic examination of the molecular distributions generated by T4 ligase-mediated joining of EcoRI-cleaved pBR322 DNA in an extension of the Jacobson-Stockmayer theory on the probability of intramolecular cyclization.
Journal ArticleDOI
Antifolate-resistant Chinese hamster cells. Molecular basis for the biochemical and structural heterogeneity among dihydrofolate reductases produced by drug-sensitive and drug-resistant cell lines.
P W Melera,J P Davide,H Oen +2 more
TL;DR: In vitro translation experiments show that the minor pI forms of DHFR known to exist in Chinese hamster lung cells are generated by a translational or post-translational modification step, and preliminary evidence suggests that this modification may result from an acetylation of the N terminus of the protein.
References
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Journal ArticleDOI
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Francisco Bolívar,Raymond L. Rodriguez,Patricia J. Greene,Mary C. Betlach,Herbert L. Heyneker,Herbert W. Boyer,Jorge H. Crosa,Stanley Falkow +7 more
TL;DR: In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).
Journal ArticleDOI
Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNA
TL;DR: Covalently-closed, catenated, and open (nicked) circular forms of R-factor DNA are all effective in transformation, but denaturation and sonication abolish the transforming ability of R.factor DNA in this system.
Journal ArticleDOI
Calcium-dependent bacteriophage DNA infection.
Manley Mandel,A. Higa +1 more
TL;DR: Escherichia coli cells of strain K12 and C can be made competent to take up temperate phage DNA without the use of “helper phage”, and is effective for both linear and circular DNA molecules.