Journal ArticleDOI
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
M. Dagert,S.D. Ehrlich +1 more
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Escherichia coli cells are 4--6 times more transformable and 20--30 times more competent after 24 h incubation in cold calcium chloride than immediately after calcium chloride treatment.About:
This article is published in Gene.The article was published on 1979-01-01. It has received 1354 citations till now. The article focuses on the topics: Calcium & Incubation.read more
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Regulation of prolactin production and cell growth by estradiol: difference in sensitivity to estradiol occurs at level of messenger ribonucleic acid accumulation.
TL;DR: Data indicate that a difference in sensitivity to estrogen occurs at the level of mRNA accumulation as well as at thelevel of the biological responses, which is unlikely to account for the difference in the concentrations required to elicit the two effects.
Journal ArticleDOI
Intermolecular recombination during transformation of Bacillus subtilis competent cells by monomeric and dimeric plasmids.
TL;DR: The results indicate that dimeric and monomeric plasmid molecules are processed differently during transformation of B. subtilis competent cells.
Journal ArticleDOI
70-Kilodalton heat shock polypeptides from rainbow trout: characterization of cDNA sequences.
TL;DR: Two derived trout hsp70 polypeptide sequences show extensive homology with derived amino acid sequences for hsp60 polypePTides from Drosophila melanogaster and Saccharomyces cerevisiae, and trout hSp70 cDNA sequences cross-hybridized with restriction fragments in genomic DNA from HeLa cells, bovine liver, Caenorhabditis elegans, and D. melanogasters.
Journal ArticleDOI
Sequence analysis of the nucleocapsid protein gene of human coronavirus 229E.
TL;DR: The 3′-noncoding region of the genome contains an 11-nucleotide sequence, which is relatively conserved throughout the Coronavirus family and lends support to the theory that this region is important for the replication of negative-strand RNA.
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A re-examination of the pathway for ornithine biosynthesis in a thermophilic and two mesophilic Bacillus species
Vehary Sakanyan,Anahit Kochikyan,Igor Mett,Christianne Legrain,Daniel Charlier,André Piérard,Nicolas Glansdorff +6 more
TL;DR: The expression of Bacillus stearothermophilus genes complementing arginine auxotrophs of Escherichia coli was studied and it was found that these organisms consequently use the cyclic pathway of ornithine biosynthesis.
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Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Francisco Bolívar,Raymond L. Rodriguez,Patricia J. Greene,Mary C. Betlach,Herbert L. Heyneker,Herbert W. Boyer,Jorge H. Crosa,Stanley Falkow +7 more
TL;DR: In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).
Journal ArticleDOI
Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNA
TL;DR: Covalently-closed, catenated, and open (nicked) circular forms of R-factor DNA are all effective in transformation, but denaturation and sonication abolish the transforming ability of R.factor DNA in this system.
Journal ArticleDOI
Calcium-dependent bacteriophage DNA infection.
Manley Mandel,A. Higa +1 more
TL;DR: Escherichia coli cells of strain K12 and C can be made competent to take up temperate phage DNA without the use of “helper phage”, and is effective for both linear and circular DNA molecules.