Journal ArticleDOI
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
M. Dagert,S.D. Ehrlich +1 more
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Escherichia coli cells are 4--6 times more transformable and 20--30 times more competent after 24 h incubation in cold calcium chloride than immediately after calcium chloride treatment.About:
This article is published in Gene.The article was published on 1979-01-01. It has received 1354 citations till now. The article focuses on the topics: Calcium & Incubation.read more
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Amplification of resistance genes in Haemophilus influenzae plasmids.
TL;DR: Evaluated results suggest that AS is responsible for the recombinational events involved in the gene amplification process, and represents a general constituent of the H. influenzae plasmids of the 45-kb class.
Journal ArticleDOI
Identification and characterization of Salmonella enterica subsp. enterica serovar Derby isolates carrying a new aadA26 gene cassette in a class 1 integron obtained at pig slaughterhouses.
Graciela Volz Lopes,Graciela Volz Lopes,Geovana Brenner Michael,Marisa Ribeiro de Itapema Cardoso,Stefan Schwarz +4 more
TL;DR: The close relationships among these isolates and isolates from an earlier study suggest the persistence of a resistant clone of S. Derby in the pig production chain in Southern Brazil.
Journal ArticleDOI
Transfer of the Ti Plasmid from Agrobacterium tumefaciens into Escherichia coli Cells
Michael Sprinzl,Klaus Geider +1 more
TL;DR: The results show that A. tumefaciens can transfer the Ti plasmid to E. coli with the same efficiency as within its own species, including hosts which do not replicate the transferred DNA.
Journal ArticleDOI
Transformation of Rhodopseudomonas sphaeroides with deoxyribonucleic acid isolated from bacteriophage R phi 6P.
W T Tucker,J M Pemberton +1 more
TL;DR: The transformation of the photosynthetic bacterium Rhodopseudomonas sphaeroides with the circular genome of the penicillinase-encoding, temperate bacteriophage R phi 6P was demonstrated.
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Concerted evolution led to high expression of a prosimian primate delta globin gene locus.
TL;DR: In the present study, the nucleotide and amino acid sequences of the galago delta and beta globin genes and their encoded peptides were determined, and evidence is provided showing that the Galago delta locus encodes a beta-type chain found in 40% of theGalago fetal and postnatal hemoglobin molecules, whereas the beta locusencodes the remaining 60%" of the beta- type chain (beta 1).
References
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Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Francisco Bolívar,Raymond L. Rodriguez,Patricia J. Greene,Mary C. Betlach,Herbert L. Heyneker,Herbert W. Boyer,Jorge H. Crosa,Stanley Falkow +7 more
TL;DR: In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).
Journal ArticleDOI
Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNA
TL;DR: Covalently-closed, catenated, and open (nicked) circular forms of R-factor DNA are all effective in transformation, but denaturation and sonication abolish the transforming ability of R.factor DNA in this system.
Journal ArticleDOI
Calcium-dependent bacteriophage DNA infection.
Manley Mandel,A. Higa +1 more
TL;DR: Escherichia coli cells of strain K12 and C can be made competent to take up temperate phage DNA without the use of “helper phage”, and is effective for both linear and circular DNA molecules.