Journal ArticleDOI
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
M. Dagert,S.D. Ehrlich +1 more
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Escherichia coli cells are 4--6 times more transformable and 20--30 times more competent after 24 h incubation in cold calcium chloride than immediately after calcium chloride treatment.About:
This article is published in Gene.The article was published on 1979-01-01. It has received 1354 citations till now. The article focuses on the topics: Calcium & Incubation.read more
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Acquired Resistance to Trimethoprim-Sulfamethoxazole during Whipple Disease and Expression of the Causative Target Gene
TL;DR: Antibiotic susceptibility of fastidious bacteria such as T. whipplei can be evaluated by means of gene complementation techniques, which showed that the gene encoding putative DHPS restored the folate biosynthesis pathway and susceptibility to sulfamethoxazole, whereas the mutated sequence was associated with sulfamETHXazole resistance.
Journal ArticleDOI
A 2.6 kb DNA sequence of Streptomyces coelicolor A3(2) which functions as a transposable element.
TL;DR: The mini-circle sequence is absent from S. lividans 66 and S. violaceolatus ISP5438 and from several Streptomyces species less closely related to S. coelicolor A3(2).
Journal ArticleDOI
Recombination between short repeated sequences is more frequent in plasmids than in the chromosome of Bacillus subtilis
L. Jannière,S. D. Ehrlich +1 more
TL;DR: The recombination frequency between directly repeated 9 bp sequences flanking the transposon Tn5 is measured in Bacillus subtilis and it is proposed that this difference is due to the generation of single stranded DNA during plasmid replication.
Journal ArticleDOI
Expression of a bean storage protein 'phaseolin minigene' in foreign plant tissues
TL;DR: Results demonstrate that phaseolin gene introns and intron splicing are not a necessary requirement for biogenesis of stable phaseolin mRNA and that no alternative splice site was introduced by the removal of five introns.
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Essential Nature of the mreC Determinant of Bacillus subtilis
Joong-Chul Lee,George C. Stewart +1 more
TL;DR: It was shown that cells lacking sufficient quantities of MreC undergo morphological changes, namely, swelling and twisting of the cells, which is followed by cell lysis, and that lysis results from a deficiency in long-axis peptidoglycan synthesis.
References
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Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Francisco Bolívar,Raymond L. Rodriguez,Patricia J. Greene,Mary C. Betlach,Herbert L. Heyneker,Herbert W. Boyer,Jorge H. Crosa,Stanley Falkow +7 more
TL;DR: In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).
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Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNA
TL;DR: Covalently-closed, catenated, and open (nicked) circular forms of R-factor DNA are all effective in transformation, but denaturation and sonication abolish the transforming ability of R.factor DNA in this system.
Journal ArticleDOI
Calcium-dependent bacteriophage DNA infection.
Manley Mandel,A. Higa +1 more
TL;DR: Escherichia coli cells of strain K12 and C can be made competent to take up temperate phage DNA without the use of “helper phage”, and is effective for both linear and circular DNA molecules.