Journal ArticleDOI
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
M. Dagert,S.D. Ehrlich +1 more
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Escherichia coli cells are 4--6 times more transformable and 20--30 times more competent after 24 h incubation in cold calcium chloride than immediately after calcium chloride treatment.About:
This article is published in Gene.The article was published on 1979-01-01. It has received 1354 citations till now. The article focuses on the topics: Calcium & Incubation.read more
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Cloning of Clostridium acetobutylicum genes and their expression in Escherichia coli and Bacillus subtilis
Irène Efstathiou,Nicole Truffaut +1 more
TL;DR: A collection of ampicillin-resistant, tetracycline-sensitive clones representative of the Clostridium acetobutylicum genome was made and the cloned sequences were shown by Southern blot hybridization to be homologous to the corresponding ABKn8 DNA fragments.
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Chorismate mutase of Thermus thermophilus is a monofunctional AroH class enzyme inhibited by tyrosine
TL;DR: Comparison of the crystal structure ofaroG, encoding the monofunctional chorismate mutase (TtCM) of the thermophilic gram-negative bacterium Thermus thermophilus, revealed an increase in hydrophilicity on the protein’s surface, greater hydrophobicity in cavities within the protein, and greater restriction of conformational freedom, features that contribute to the thermal stability of this choristic mutase.
Journal ArticleDOI
DNA sequence of the purC gene encoding 5'-phosphoribosyl-5-aminoimidazole-4-N-succinocarboxamide synthetase and organization of the dapA-purC region of Escherichia coli K-12.
TL;DR: The purC gene has been positioned on the E. coli restriction map and is transcribed in a counterclockwise direction, and an unusual feature of the 5' untranslated region of the purC mRNA is the presence of a repetitive extragenic palindrome sequence normally found in intercistronic or 3'Untranslated regions.
Journal ArticleDOI
Azotobacter vinelandii flavodoxin : purification and properties of the recombinant, dephospho form expressed in Escherichia coli
TL;DR: The nifF gene coding for the flavodoxin from the nitrogen-fixing bacterium Azotobacter vinelandii was cloned into the plasmid vector pUC7 and the resulting plasmids transformed and expressed in Escherichia coli strain DH5, resulting in an apoflavodoxin that was much less stable than that prepared from the native protein.
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Cross-reactions between engineered xylose and galactose pathways in recombinant Saccharomyces cerevisiae.
TL;DR: PGM2 overexpression was shown to benefit xylose and galactose fermentation, alone and in combination and cross-reactions are of particular relevance for the fermentation of mixed sugars from lignocellulosic feedstock.
References
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Journal ArticleDOI
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Francisco Bolívar,Raymond L. Rodriguez,Patricia J. Greene,Mary C. Betlach,Herbert L. Heyneker,Herbert W. Boyer,Jorge H. Crosa,Stanley Falkow +7 more
TL;DR: In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).
Journal ArticleDOI
Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNA
TL;DR: Covalently-closed, catenated, and open (nicked) circular forms of R-factor DNA are all effective in transformation, but denaturation and sonication abolish the transforming ability of R.factor DNA in this system.
Journal ArticleDOI
Calcium-dependent bacteriophage DNA infection.
Manley Mandel,A. Higa +1 more
TL;DR: Escherichia coli cells of strain K12 and C can be made competent to take up temperate phage DNA without the use of “helper phage”, and is effective for both linear and circular DNA molecules.