Journal ArticleDOI
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
M. Dagert,S.D. Ehrlich +1 more
Reads0
Chats0
TLDR
Escherichia coli cells are 4--6 times more transformable and 20--30 times more competent after 24 h incubation in cold calcium chloride than immediately after calcium chloride treatment.About:
This article is published in Gene.The article was published on 1979-01-01. It has received 1354 citations till now. The article focuses on the topics: Calcium & Incubation.read more
Citations
More filters
Journal ArticleDOI
Molecular cloning and characterization of the genes (pbpA and rodA) responsible for the rod shape of Escherichia coli K-12: analysis of gene expression with transposon Tn5 mutagenesis and protein synthesis directed by constructed plasmids.
TL;DR: Two cell shape-determining genes of Escherichia coli K-12, pbpA, the structural gene for penicillin-binding protein 2, and rodA, whose protein is unknown, were subcloned into plasmid vectors from the transducing phage lambda MAd lip24, which carries the lip-leuS region of the E. coli chromosome.
Journal ArticleDOI
Conjugative multiple‐antibiotic resistance plasmids in Escherichia coli isolated from environmental waters contaminated by human faecal wastes
E. Laroche-Ajzenberg,A. Flores Ribeiro,Josselin Bodilis,W. Riah,Sylvaine Buquet,N. Chaftar,Barbara Pawlak +6 more
TL;DR: The genetic supports of resistances in nine multi‐resistant Escherichia coli strains originating from human faecal contamination, and isolated from three different aquatic environments used for producing drinking water, are investigated.
Journal ArticleDOI
Assembly of the aspartate transcarbamoylase holoenzyme from transcriptionally independent catalytic and regulatory cistrons.
TL;DR: The cistrons encoding the regulatory and catalytic polypeptides of aspartate transcarbamoylase from Escherichia coli K-12 have been cloned separately on plasmids from different incompatability groups and it is clear that the in vivo assembly of ATCase holoenzyme is a direct, spontaneous process involving the association of preformed regulatory subunits (r2) and catalytes (c3).
Book ChapterDOI
Glycoprotein E1 of MHV-A59: structure of the O-linked carbohydrates and construction of full length recombinant cDNA clones.
TL;DR: The murine coronaviruses contain two different classes of glycoproteins, and the envelope glycoprotein E2 resembles in its composition and biosynthetic processing the classical type of viral glycop protein.
Journal ArticleDOI
A novel salt-inducible vector for efficient expression and secretion of heterologous proteins in Bacillus subtilis
Ruangurai Promchai,Boonhiang Promdonkoy,Sutipa Tanapongpipat,Wonnop Visessanguan,Lily Eurwilaichitr,Plearnpis Luxananil +5 more
TL;DR: The results demonstrated that pSaltExSePR5 provides an alternative vector for efficient and simple production of heterologous proteins in B. subtilis with a safer and more economic inducer.
References
More filters
Journal ArticleDOI
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Francisco Bolívar,Raymond L. Rodriguez,Patricia J. Greene,Mary C. Betlach,Herbert L. Heyneker,Herbert W. Boyer,Jorge H. Crosa,Stanley Falkow +7 more
TL;DR: In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).
Journal ArticleDOI
Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNA
TL;DR: Covalently-closed, catenated, and open (nicked) circular forms of R-factor DNA are all effective in transformation, but denaturation and sonication abolish the transforming ability of R.factor DNA in this system.
Journal ArticleDOI
Calcium-dependent bacteriophage DNA infection.
Manley Mandel,A. Higa +1 more
TL;DR: Escherichia coli cells of strain K12 and C can be made competent to take up temperate phage DNA without the use of “helper phage”, and is effective for both linear and circular DNA molecules.